Gene Essentiality: Methods and Protocols
This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Schriftenreihe: | Methods in Molecular Biology
1279 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study |
| Beschreibung: | 1 Online-Ressource (XI, 248 p. 42 illus., 15 illus. in color) |
| ISBN: | 9781493923984 |
| DOI: | 10.1007/978-1-4939-2398-4 |
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| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-2398-4 |
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| spelling | Gene Essentiality Methods and Protocols edited by Long Jason Lu New York, NY Springer New York 2015 1 Online-Ressource (XI, 248 p. 42 illus., 15 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1279 This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study Biomedicine Human Genetics Microbial Genetics and Genomics Medicine Human genetics Microbial genetics Microbial genomics Nachweis (DE-588)4115334-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Gen (DE-588)4128987-0 s Nachweis (DE-588)4115334-0 s Methode (DE-588)4038971-6 s 2\p DE-604 Lu, Long Jason edt Erscheint auch als Druck-Ausgabe 9781493923977 https://doi.org/10.1007/978-1-4939-2398-4 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gene Essentiality Methods and Protocols Biomedicine Human Genetics Microbial Genetics and Genomics Medicine Human genetics Microbial genetics Microbial genomics Nachweis (DE-588)4115334-0 gnd Methode (DE-588)4038971-6 gnd Gen (DE-588)4128987-0 gnd |
| subject_GND | (DE-588)4115334-0 (DE-588)4038971-6 (DE-588)4128987-0 (DE-588)4143413-4 |
| title | Gene Essentiality Methods and Protocols |
| title_auth | Gene Essentiality Methods and Protocols |
| title_exact_search | Gene Essentiality Methods and Protocols |
| title_full | Gene Essentiality Methods and Protocols edited by Long Jason Lu |
| title_fullStr | Gene Essentiality Methods and Protocols edited by Long Jason Lu |
| title_full_unstemmed | Gene Essentiality Methods and Protocols edited by Long Jason Lu |
| title_short | Gene Essentiality |
| title_sort | gene essentiality methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Microbial Genetics and Genomics Medicine Human genetics Microbial genetics Microbial genomics Nachweis (DE-588)4115334-0 gnd Methode (DE-588)4038971-6 gnd Gen (DE-588)4128987-0 gnd |
| topic_facet | Biomedicine Human Genetics Microbial Genetics and Genomics Medicine Human genetics Microbial genetics Microbial genomics Nachweis Methode Gen Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-2398-4 |
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