Regulatory B Cells: Methods and Protocols
Regulatory B Cells: Methods and Protocols presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detail...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2014
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1190 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Regulatory B Cells: Methods and Protocols presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology |
| Beschreibung: | 1 Online-Ressource (XI, 292 p. 53 illus., 19 illus. in color) |
| ISBN: | 9781493911615 |
| DOI: | 10.1007/978-1-4939-1161-5 |
Internformat
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Datensatz im Suchindex
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| spelling | Regulatory B Cells Methods and Protocols edited by Gaetano Vitale, Francesca Mion New York, NY Springer New York 2014 1 Online-Ressource (XI, 292 p. 53 illus., 19 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1190 Regulatory B Cells: Methods and Protocols presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology Biomedicine Immunology Cell Culture Medicine Cell culture B-Lymphozyt (DE-588)4137121-5 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf B-Lymphozyt (DE-588)4137121-5 s Labortechnik (DE-588)4123602-6 s b DE-604 Vitale, Gaetano edt Mion, Francesca edt Erscheint auch als Druck-Ausgabe 9781493911608 https://doi.org/10.1007/978-1-4939-1161-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Regulatory B Cells Methods and Protocols Biomedicine Immunology Cell Culture Medicine Cell culture B-Lymphozyt (DE-588)4137121-5 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4137121-5 (DE-588)4123602-6 |
| title | Regulatory B Cells Methods and Protocols |
| title_auth | Regulatory B Cells Methods and Protocols |
| title_exact_search | Regulatory B Cells Methods and Protocols |
| title_full | Regulatory B Cells Methods and Protocols edited by Gaetano Vitale, Francesca Mion |
| title_fullStr | Regulatory B Cells Methods and Protocols edited by Gaetano Vitale, Francesca Mion |
| title_full_unstemmed | Regulatory B Cells Methods and Protocols edited by Gaetano Vitale, Francesca Mion |
| title_short | Regulatory B Cells |
| title_sort | regulatory b cells methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Immunology Cell Culture Medicine Cell culture B-Lymphozyt (DE-588)4137121-5 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Biomedicine Immunology Cell Culture Medicine Cell culture B-Lymphozyt Labortechnik |
| url | https://doi.org/10.1007/978-1-4939-1161-5 |
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