DNA methylation protocols:

This third edition volume expands on the previous editions with a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using...

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Bibliographic Details
Other Authors: Tost, Jörg (Editor)
Format: Electronic eBook
Language:English
Published: New York, NY Springer New York 2018
Edition:3rd ed. 2018
Series:Methods in Molecular Biology 1708
Subjects:
Online Access:UBR01
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Summary:This third edition volume expands on the previous editions with a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reag ents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components
Physical Description:1 Online-Ressource (XVIII, 704 Seiten) Illustrationen
ISBN:9781493974818
DOI:10.1007/978-1-4939-7481-8

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