Cardiac Gene Expression: Methods and Protocols
Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes...
Gespeichert in:
Weitere Verfasser: | , |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2007
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Schriftenreihe: | Methods in Molecular Biology
366 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion |
Beschreibung: | 1 Online-Ressource (XII, 366 p. 66 illus., 1 illus. in color) |
ISBN: | 9781597450300 |
DOI: | 10.1007/978-1-59745-030-0 |
Internformat
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520 | |a Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion | ||
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Datensatz im Suchindex
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any_adam_object | |
author2 | Zhang, Jun Rokosh, Gregg |
author2_role | edt edt |
author2_variant | j z jz g r gr |
author_facet | Zhang, Jun Rokosh, Gregg |
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dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572 |
dewey-search | 572 |
dewey-sort | 3572 |
dewey-tens | 570 - Biology |
discipline | Biologie |
doi_str_mv | 10.1007/978-1-59745-030-0 |
format | Electronic eBook |
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id | DE-604.BV044952079 |
illustrated | Not Illustrated |
indexdate | 2024-07-10T08:05:37Z |
institution | BVB |
isbn | 9781597450300 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344835 |
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physical | 1 Online-Ressource (XII, 366 p. 66 illus., 1 illus. in color) |
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publisher | Humana Press |
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series2 | Methods in Molecular Biology |
spelling | Cardiac Gene Expression Methods and Protocols edited by Jun Zhang, Gregg Rokosh Totowa, NJ Humana Press 2007 1 Online-Ressource (XII, 366 p. 66 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 366 Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion Life Sciences Biochemistry, general Human Genetics Cardiology Bioinformatics Life sciences Human genetics Biochemistry Zhang, Jun edt Rokosh, Gregg edt Erscheint auch als Druck-Ausgabe 9781588293527 https://doi.org/10.1007/978-1-59745-030-0 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Cardiac Gene Expression Methods and Protocols Life Sciences Biochemistry, general Human Genetics Cardiology Bioinformatics Life sciences Human genetics Biochemistry |
title | Cardiac Gene Expression Methods and Protocols |
title_auth | Cardiac Gene Expression Methods and Protocols |
title_exact_search | Cardiac Gene Expression Methods and Protocols |
title_full | Cardiac Gene Expression Methods and Protocols edited by Jun Zhang, Gregg Rokosh |
title_fullStr | Cardiac Gene Expression Methods and Protocols edited by Jun Zhang, Gregg Rokosh |
title_full_unstemmed | Cardiac Gene Expression Methods and Protocols edited by Jun Zhang, Gregg Rokosh |
title_short | Cardiac Gene Expression |
title_sort | cardiac gene expression methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Biochemistry, general Human Genetics Cardiology Bioinformatics Life sciences Human genetics Biochemistry |
topic_facet | Life Sciences Biochemistry, general Human Genetics Cardiology Bioinformatics Life sciences Human genetics Biochemistry |
url | https://doi.org/10.1007/978-1-59745-030-0 |
work_keys_str_mv | AT zhangjun cardiacgeneexpressionmethodsandprotocols AT rokoshgregg cardiacgeneexpressionmethodsandprotocols |