Human Cell Culture Protocols:
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previ...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2012
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
806 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research |
| Beschreibung: | 1 Online-Ressource (XIV, 435 p. 89 illus., 33 illus. in color) |
| ISBN: | 9781617793677 |
| DOI: | 10.1007/978-1-61779-367-7 |
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Datensatz im Suchindex
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| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
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| dewey-search | 571.6 |
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| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-61779-367-7 |
| format | Electronic eBook |
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| indexdate | 2024-07-10T08:05:37Z |
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| spelling | Human Cell Culture Protocols edited by Ragai R. Mitry, Robin D. Hughes Totowa, NJ Humana Press 2012 1 Online-Ressource (XIV, 435 p. 89 illus., 33 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 806 Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research Life Sciences Cell Biology Cell Culture Life sciences Cell biology Cell culture Mitry, Ragai R. edt Hughes, Robin D. edt Erscheint auch als Druck-Ausgabe 9781617793660 https://doi.org/10.1007/978-1-61779-367-7 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Human Cell Culture Protocols Life Sciences Cell Biology Cell Culture Life sciences Cell biology Cell culture |
| title | Human Cell Culture Protocols |
| title_auth | Human Cell Culture Protocols |
| title_exact_search | Human Cell Culture Protocols |
| title_full | Human Cell Culture Protocols edited by Ragai R. Mitry, Robin D. Hughes |
| title_fullStr | Human Cell Culture Protocols edited by Ragai R. Mitry, Robin D. Hughes |
| title_full_unstemmed | Human Cell Culture Protocols edited by Ragai R. Mitry, Robin D. Hughes |
| title_short | Human Cell Culture Protocols |
| title_sort | human cell culture protocols |
| topic | Life Sciences Cell Biology Cell Culture Life sciences Cell biology Cell culture |
| topic_facet | Life Sciences Cell Biology Cell Culture Life sciences Cell biology Cell culture |
| url | https://doi.org/10.1007/978-1-61779-367-7 |
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