Receptor Binding Techniques:
A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine fac...
Saved in:
| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2012
|
| Edition: | 3rd ed. 2012 |
| Series: | Methods in Molecular Biology, Methods and Protocols
897 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding |
| Physical Description: | 1 Online-Ressource (XII, 309 p. 70 illus., 30 illus. in color) |
| ISBN: | 9781617799099 |
| DOI: | 10.1007/978-1-61779-909-9 |
Staff View
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951950 | ||
| 003 | DE-604 | ||
| 005 | 20181015 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2012 |||| o||u| ||||||eng d | ||
| 020 | |a 9781617799099 |9 978-1-61779-909-9 | ||
| 024 | 7 | |a 10.1007/978-1-61779-909-9 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-61779-909-9 | ||
| 035 | |a (OCoLC)808342937 | ||
| 035 | |a (DE-599)BVBBV044951950 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 572.6 |2 23 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WE 5200 |0 (DE-625)148300: |2 rvk | ||
| 245 | 1 | 0 | |a Receptor Binding Techniques |c edited by Anthony P. Davenport |
| 250 | |a 3rd ed. 2012 | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2012 | |
| 300 | |a 1 Online-Ressource (XII, 309 p. 70 illus., 30 illus. in color) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology, Methods and Protocols |v 897 | |
| 520 | |a A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Protein Science | |
| 650 | 4 | |a Protein-Ligand Interactions | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Proteins | |
| 650 | 0 | 7 | |a Wirkstoff-Rezeptor-Bindung |0 (DE-588)4190019-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biochemische Methode |0 (DE-588)4319880-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Wirkstoff-Rezeptor-Bindung |0 (DE-588)4190019-4 |D s |
| 689 | 0 | 1 | |a Biochemische Methode |0 (DE-588)4319880-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Davenport, Anthony P. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781617799082 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-61779-909-9 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344706 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1007/978-1-61779-909-9 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-61779-909-9 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Record in the Search Index
| _version_ | 1804178541230686208 |
|---|---|
| any_adam_object | |
| author2 | Davenport, Anthony P. |
| author2_role | edt |
| author2_variant | a p d ap apd |
| author_facet | Davenport, Anthony P. |
| building | Verbundindex |
| bvnumber | BV044951950 |
| classification_rvk | WC 4150 WE 5200 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-61779-909-9 (OCoLC)808342937 (DE-599)BVBBV044951950 |
| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-61779-909-9 |
| edition | 3rd ed. 2012 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03936nmm a2200553zcb4500</leader><controlfield tag="001">BV044951950</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20181015 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2012 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781617799099</subfield><subfield code="9">978-1-61779-909-9</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-61779-909-9</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-61779-909-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)808342937</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951950</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WE 5200</subfield><subfield code="0">(DE-625)148300:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Receptor Binding Techniques</subfield><subfield code="c">edited by Anthony P. Davenport</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">3rd ed. 2012</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XII, 309 p. 70 illus., 30 illus. in color)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology, Methods and Protocols</subfield><subfield code="v">897</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Protein Science</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Protein-Ligand Interactions</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Wirkstoff-Rezeptor-Bindung</subfield><subfield code="0">(DE-588)4190019-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biochemische Methode</subfield><subfield code="0">(DE-588)4319880-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Wirkstoff-Rezeptor-Bindung</subfield><subfield code="0">(DE-588)4190019-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Biochemische Methode</subfield><subfield code="0">(DE-588)4319880-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Davenport, Anthony P.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781617799082</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-61779-909-9</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344706</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-61779-909-9</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-61779-909-9</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044951950 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9781617799099 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344706 |
| oclc_num | 808342937 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XII, 309 p. 70 illus., 30 illus. in color) |
| psigel | ZDB-2-PRO |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology, Methods and Protocols |
| spelling | Receptor Binding Techniques edited by Anthony P. Davenport 3rd ed. 2012 Totowa, NJ Humana Press 2012 1 Online-Ressource (XII, 309 p. 70 illus., 30 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 897 A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding Life Sciences Protein Science Protein-Ligand Interactions Life sciences Proteins Wirkstoff-Rezeptor-Bindung (DE-588)4190019-4 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Wirkstoff-Rezeptor-Bindung (DE-588)4190019-4 s Biochemische Methode (DE-588)4319880-6 s b DE-604 Davenport, Anthony P. edt Erscheint auch als Druck-Ausgabe 9781617799082 https://doi.org/10.1007/978-1-61779-909-9 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Receptor Binding Techniques Life Sciences Protein Science Protein-Ligand Interactions Life sciences Proteins Wirkstoff-Rezeptor-Bindung (DE-588)4190019-4 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| subject_GND | (DE-588)4190019-4 (DE-588)4319880-6 (DE-588)4143413-4 |
| title | Receptor Binding Techniques |
| title_auth | Receptor Binding Techniques |
| title_exact_search | Receptor Binding Techniques |
| title_full | Receptor Binding Techniques edited by Anthony P. Davenport |
| title_fullStr | Receptor Binding Techniques edited by Anthony P. Davenport |
| title_full_unstemmed | Receptor Binding Techniques edited by Anthony P. Davenport |
| title_short | Receptor Binding Techniques |
| title_sort | receptor binding techniques |
| topic | Life Sciences Protein Science Protein-Ligand Interactions Life sciences Proteins Wirkstoff-Rezeptor-Bindung (DE-588)4190019-4 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| topic_facet | Life Sciences Protein Science Protein-Ligand Interactions Life sciences Proteins Wirkstoff-Rezeptor-Bindung Biochemische Methode Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-61779-909-9 |
| work_keys_str_mv | AT davenportanthonyp receptorbindingtechniques |