Nucleic Acid Crystallography: Methods and Protocols

This volume includes comprehensive and up-to-date coverage of all nucleic acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused o...

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Weitere Verfasser: Ennifar, Eric (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: New York, NY Springer New York 2016
Ausgabe:1st ed. 2016
Schriftenreihe:Methods in Molecular Biology 1320
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Zusammenfassung:This volume includes comprehensive and up-to-date coverage of all nucleic acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.  
Beschreibung:1 Online-Ressource (XI, 356 p. 116 illus., 98 illus. in color)
ISBN:9781493927630
DOI:10.1007/978-1-4939-2763-0