The Nucleus:
This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei,...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Ausgabe: | 2nd ed. 2015 |
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1228 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus |
| Beschreibung: | 1 Online-Ressource (XI, 237 p. 54 illus., 35 illus. in color) |
| ISBN: | 9781493916801 |
| DOI: | 10.1007/978-1-4939-1680-1 |
Internformat
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| discipline | Biologie |
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| edition | 2nd ed. 2015 |
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| spelling | The Nucleus edited by Ronald Hancock 2nd ed. 2015 New York, NY Springer New York 2015 1 Online-Ressource (XI, 237 p. 54 illus., 35 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1228 This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus Life Sciences Cell Biology Life sciences Cell biology Zellkern (DE-588)4067544-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellkern (DE-588)4067544-0 s Methode (DE-588)4038971-6 s b DE-604 Hancock, Ronald edt Erscheint auch als Druck-Ausgabe 9781493916795 https://doi.org/10.1007/978-1-4939-1680-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | The Nucleus Life Sciences Cell Biology Life sciences Cell biology Zellkern (DE-588)4067544-0 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4067544-0 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | The Nucleus |
| title_auth | The Nucleus |
| title_exact_search | The Nucleus |
| title_full | The Nucleus edited by Ronald Hancock |
| title_fullStr | The Nucleus edited by Ronald Hancock |
| title_full_unstemmed | The Nucleus edited by Ronald Hancock |
| title_short | The Nucleus |
| title_sort | the nucleus |
| topic | Life Sciences Cell Biology Life sciences Cell biology Zellkern (DE-588)4067544-0 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Zellkern Methode Aufsatzsammlung |
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