Directed Enzyme Evolution: Screening and Selection Methods
Directed evolution, the application of evolutionary design to enzyme engineering, requires effective screening strategies to isolate those proteins that perform a desired function from the libraries generated by the techniques. In Directed Enzyme Evolution: Screening and Selection Methods, seasoned...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Schriftenreihe: | Methods in Molecular Biology™
230 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Directed evolution, the application of evolutionary design to enzyme engineering, requires effective screening strategies to isolate those proteins that perform a desired function from the libraries generated by the techniques. In Directed Enzyme Evolution: Screening and Selection Methods, seasoned practitioners from many leading laboratories describe their leading and readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. There are also representative examples of how phage libraries may be interrogated for enzymatic activity. Each protocol contains detailed step-by-step instructions and many notes on how best to deal with the problems that may occur. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Taken together, Directed Enzyme Evolution: Screening and Selection Methods and Directed Evolution Library Creation: Methods and Protocols capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications |
| Beschreibung: | 1 Online-Ressource (XV, 383 p) |
| ISBN: | 9781592593965 |
| DOI: | 10.1385/1592593968 |
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| spelling | Directed Enzyme Evolution Screening and Selection Methods edited by Frances H. Arnold, George Georgiou Totowa, NJ Humana Press 2003 1 Online-Ressource (XV, 383 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 230 Directed evolution, the application of evolutionary design to enzyme engineering, requires effective screening strategies to isolate those proteins that perform a desired function from the libraries generated by the techniques. In Directed Enzyme Evolution: Screening and Selection Methods, seasoned practitioners from many leading laboratories describe their leading and readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. There are also representative examples of how phage libraries may be interrogated for enzymatic activity. Each protocol contains detailed step-by-step instructions and many notes on how best to deal with the problems that may occur. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Taken together, Directed Enzyme Evolution: Screening and Selection Methods and Directed Evolution Library Creation: Methods and Protocols capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications Life Sciences Cell Biology Life sciences Cell biology Enzym (DE-588)4014988-2 gnd rswk-swf Molekulardesign (DE-588)4265444-0 gnd rswk-swf Ortspezifische Mutagenese (DE-588)4312683-2 gnd rswk-swf Enzymdiagnostik (DE-588)4133537-5 gnd rswk-swf Proteindesign (DE-588)4346801-9 gnd rswk-swf Auslese (DE-588)4003800-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Enzym (DE-588)4014988-2 s Molekulardesign (DE-588)4265444-0 s DE-604 Proteindesign (DE-588)4346801-9 s Enzymdiagnostik (DE-588)4133537-5 s Ortspezifische Mutagenese (DE-588)4312683-2 s Auslese (DE-588)4003800-2 s b DE-604 Arnold, Frances H. edt Georgiou, George edt Erscheint auch als Druck-Ausgabe 9781588292865 https://doi.org/10.1385/1592593968 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Directed Enzyme Evolution Screening and Selection Methods Life Sciences Cell Biology Life sciences Cell biology Enzym (DE-588)4014988-2 gnd Molekulardesign (DE-588)4265444-0 gnd Ortspezifische Mutagenese (DE-588)4312683-2 gnd Enzymdiagnostik (DE-588)4133537-5 gnd Proteindesign (DE-588)4346801-9 gnd Auslese (DE-588)4003800-2 gnd |
| subject_GND | (DE-588)4014988-2 (DE-588)4265444-0 (DE-588)4312683-2 (DE-588)4133537-5 (DE-588)4346801-9 (DE-588)4003800-2 (DE-588)4143413-4 |
| title | Directed Enzyme Evolution Screening and Selection Methods |
| title_auth | Directed Enzyme Evolution Screening and Selection Methods |
| title_exact_search | Directed Enzyme Evolution Screening and Selection Methods |
| title_full | Directed Enzyme Evolution Screening and Selection Methods edited by Frances H. Arnold, George Georgiou |
| title_fullStr | Directed Enzyme Evolution Screening and Selection Methods edited by Frances H. Arnold, George Georgiou |
| title_full_unstemmed | Directed Enzyme Evolution Screening and Selection Methods edited by Frances H. Arnold, George Georgiou |
| title_short | Directed Enzyme Evolution |
| title_sort | directed enzyme evolution screening and selection methods |
| title_sub | Screening and Selection Methods |
| topic | Life Sciences Cell Biology Life sciences Cell biology Enzym (DE-588)4014988-2 gnd Molekulardesign (DE-588)4265444-0 gnd Ortspezifische Mutagenese (DE-588)4312683-2 gnd Enzymdiagnostik (DE-588)4133537-5 gnd Proteindesign (DE-588)4346801-9 gnd Auslese (DE-588)4003800-2 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Enzym Molekulardesign Ortspezifische Mutagenese Enzymdiagnostik Proteindesign Auslese Aufsatzsammlung |
| url | https://doi.org/10.1385/1592593968 |
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