Hydrocarbon and Lipid Microbiology Protocols: Microbial Quantitation, Community Profiling and Array Approaches
This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon...
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| Weitere Verfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2017
|
| Ausgabe: | 1st ed. 2017 |
| Schriftenreihe: | Springer Protocols Handbooks
|
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes |
| Beschreibung: | 1 Online-Ressource (X, 252 p. 30 illus., 12 illus. in color) |
| ISBN: | 9783662527788 |
| DOI: | 10.1007/978-3-662-52778-8 |
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Datensatz im Suchindex
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| any_adam_object | |
| author2 | McGenity, Terry J. Timmis, Kenneth N. Nogales, Balbina |
| author2_role | edt edt edt |
| author2_variant | t j m tj tjm k n t kn knt b n bn |
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| dewey-full | 579 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
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| dewey-search | 579 |
| dewey-sort | 3579 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-3-662-52778-8 |
| edition | 1st ed. 2017 |
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| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9783662527788 |
| language | English |
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| spelling | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales 1st ed. 2017 Berlin, Heidelberg Springer Berlin Heidelberg 2017 1 Online-Ressource (X, 252 p. 30 illus., 12 illus. in color) txt rdacontent c rdamedia cr rdacarrier Springer Protocols Handbooks This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe (DE-588)4125165-9 gnd rswk-swf Lipide (DE-588)4035873-2 gnd rswk-swf Quantitative Methode (DE-588)4232139-6 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 s Kohlenwasserstoffe (DE-588)4125165-9 s Lipide (DE-588)4035873-2 s Quantitative Methode (DE-588)4232139-6 s 1\p DE-604 McGenity, Terry J. edt Timmis, Kenneth N. edt Nogales, Balbina edt Erscheint auch als Druck-Ausgabe 9783662527764 https://doi.org/10.1007/978-3-662-52778-8 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe (DE-588)4125165-9 gnd Lipide (DE-588)4035873-2 gnd Quantitative Methode (DE-588)4232139-6 gnd Mikrobiologie (DE-588)4316357-9 gnd |
| subject_GND | (DE-588)4125165-9 (DE-588)4035873-2 (DE-588)4232139-6 (DE-588)4316357-9 |
| title | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches |
| title_auth | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches |
| title_exact_search | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches |
| title_full | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales |
| title_fullStr | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales |
| title_full_unstemmed | Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales |
| title_short | Hydrocarbon and Lipid Microbiology Protocols |
| title_sort | hydrocarbon and lipid microbiology protocols microbial quantitation community profiling and array approaches |
| title_sub | Microbial Quantitation, Community Profiling and Array Approaches |
| topic | Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe (DE-588)4125165-9 gnd Lipide (DE-588)4035873-2 gnd Quantitative Methode (DE-588)4232139-6 gnd Mikrobiologie (DE-588)4316357-9 gnd |
| topic_facet | Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe Lipide Quantitative Methode Mikrobiologie |
| url | https://doi.org/10.1007/978-3-662-52778-8 |
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