Biolistic DNA Delivery: Methods and Protocols
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity...
Saved in:
| Other Authors: | , |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2013
|
| Series: | Methods in Molecular Biology, Methods and Protocols
940 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field |
| Physical Description: | 1 Online-Ressource (XIV, 415 p. 80 illus., 33 illus. in color) |
| ISBN: | 9781627031103 |
| DOI: | 10.1007/978-1-62703-110-3 |
Staff View
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| isbn | 9781627031103 |
| language | English |
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| spelling | Biolistic DNA Delivery Methods and Protocols edited by Stephan Sudowe, Angelika B. Reske-Kunz Totowa, NJ Humana Press 2013 1 Online-Ressource (XIV, 415 p. 80 illus., 33 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 940 Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering Sudowe, Stephan edt Reske-Kunz, Angelika B. edt Erscheint auch als Druck-Ausgabe 9781627031097 https://doi.org/10.1007/978-1-62703-110-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Biolistic DNA Delivery Methods and Protocols Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering |
| title | Biolistic DNA Delivery Methods and Protocols |
| title_auth | Biolistic DNA Delivery Methods and Protocols |
| title_exact_search | Biolistic DNA Delivery Methods and Protocols |
| title_full | Biolistic DNA Delivery Methods and Protocols edited by Stephan Sudowe, Angelika B. Reske-Kunz |
| title_fullStr | Biolistic DNA Delivery Methods and Protocols edited by Stephan Sudowe, Angelika B. Reske-Kunz |
| title_full_unstemmed | Biolistic DNA Delivery Methods and Protocols edited by Stephan Sudowe, Angelika B. Reske-Kunz |
| title_short | Biolistic DNA Delivery |
| title_sort | biolistic dna delivery methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering |
| topic_facet | Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering |
| url | https://doi.org/10.1007/978-1-62703-110-3 |
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