Calcium Signaling Protocols:

In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well a...

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Bibliographic Details
Other Authors: Lambert, David G. (Editor)
Format: Electronic eBook
Language:English
Published: Totowa, NJ Humana Press 1999
Series:Methods in Molecular Biology™ 114
Subjects:
Online Access:UBR01
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Summary:In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents
Physical Description:1 Online-Ressource (XIII, 359 p. 63 illus., 2 illus. in color)
ISBN:9781592592500
DOI:10.1385/1592592503

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