Matrix Metalloproteases: Methods and Protocols
Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification...
Saved in:
| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2017
|
| Series: | Methods in Molecular Biology
1579 |
| Subjects: | |
| Online Access: | UBR01 TUM01 URL des Erstveröffentlichers |
| Summary: | Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Physical Description: | 1 Online-Ressource (XI, 314 p. 56 illus., 34 illus. in color) |
| ISBN: | 9781493968633 |
| DOI: | 10.1007/978-1-4939-6863-3 |
Staff View
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951359 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2017 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493968633 |9 978-1-4939-6863-3 | ||
| 024 | 7 | |a 10.1007/978-1-4939-6863-3 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-6863-3 | ||
| 035 | |a (OCoLC)981936287 | ||
| 035 | |a (DE-599)BVBBV044951359 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 572 |2 23 | |
| 084 | |a WD 5065 |0 (DE-625)148188: |2 rvk | ||
| 245 | 1 | 0 | |a Matrix Metalloproteases |b Methods and Protocols |c edited by Charles A. Galea |
| 264 | 1 | |a New York, NY |b Springer New York |c 2017 | |
| 300 | |a 1 Online-Ressource (XI, 314 p. 56 illus., 34 illus. in color) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology |v 1579 | |
| 520 | |a Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Biochemistry, general | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Biochemistry | |
| 700 | 1 | |a Galea, Charles A. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781493968619 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-6863-3 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344116 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-6863-3 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-6863-3 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Record in the Search Index
| _version_ | 1804178539887460352 |
|---|---|
| any_adam_object | |
| author2 | Galea, Charles A. |
| author2_role | edt |
| author2_variant | c a g ca cag |
| author_facet | Galea, Charles A. |
| building | Verbundindex |
| bvnumber | BV044951359 |
| classification_rvk | WD 5065 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-6863-3 (OCoLC)981936287 (DE-599)BVBBV044951359 |
| dewey-full | 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572 |
| dewey-search | 572 |
| dewey-sort | 3572 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-6863-3 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02661nmm a2200433zcb4500</leader><controlfield tag="001">BV044951359</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2017 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493968633</subfield><subfield code="9">978-1-4939-6863-3</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-6863-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-6863-3</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)981936287</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951359</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5065</subfield><subfield code="0">(DE-625)148188:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Matrix Metalloproteases</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Charles A. Galea</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2017</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XI, 314 p. 56 illus., 34 illus. in color)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield><subfield code="v">1579</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Galea, Charles A.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781493968619</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-6863-3</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344116</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-6863-3</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-6863-3</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044951359 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781493968633 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344116 |
| oclc_num | 981936287 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XI, 314 p. 56 illus., 34 illus. in color) |
| psigel | ZDB-2-PRO |
| publishDate | 2017 |
| publishDateSearch | 2017 |
| publishDateSort | 2017 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology |
| spelling | Matrix Metalloproteases Methods and Protocols edited by Charles A. Galea New York, NY Springer New York 2017 1 Online-Ressource (XI, 314 p. 56 illus., 34 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1579 Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field Life Sciences Biochemistry, general Life sciences Biochemistry Galea, Charles A. edt Erscheint auch als Druck-Ausgabe 9781493968619 https://doi.org/10.1007/978-1-4939-6863-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Matrix Metalloproteases Methods and Protocols Life Sciences Biochemistry, general Life sciences Biochemistry |
| title | Matrix Metalloproteases Methods and Protocols |
| title_auth | Matrix Metalloproteases Methods and Protocols |
| title_exact_search | Matrix Metalloproteases Methods and Protocols |
| title_full | Matrix Metalloproteases Methods and Protocols edited by Charles A. Galea |
| title_fullStr | Matrix Metalloproteases Methods and Protocols edited by Charles A. Galea |
| title_full_unstemmed | Matrix Metalloproteases Methods and Protocols edited by Charles A. Galea |
| title_short | Matrix Metalloproteases |
| title_sort | matrix metalloproteases methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry |
| url | https://doi.org/10.1007/978-1-4939-6863-3 |
| work_keys_str_mv | AT galeacharlesa matrixmetalloproteasesmethodsandprotocols |