Argonaute Proteins: Methods and Protocols
With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and s...
Saved in:
| Other Authors: | , |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2011
|
| Edition: | 1 |
| Series: | Methods in Molecular Biology, Methods and Protocols
725 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research |
| Physical Description: | 1 Online-Ressource (XII, 344 p) |
| ISBN: | 9781617790461 |
| DOI: | 10.1007/978-1-61779-046-1 |
Staff View
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951340 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2011 |||| o||u| ||||||eng d | ||
| 020 | |a 9781617790461 |9 978-1-61779-046-1 | ||
| 024 | 7 | |a 10.1007/978-1-61779-046-1 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-61779-046-1 | ||
| 035 | |a (OCoLC)891109516 | ||
| 035 | |a (DE-599)BVBBV044951340 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 572.6 |2 23 | |
| 084 | |a WD 5355 |0 (DE-625)148201: |2 rvk | ||
| 245 | 1 | 0 | |a Argonaute Proteins |b Methods and Protocols |c edited by Tom C. Hobman, Thomas F. Duchaine |
| 250 | |a 1 | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2011 | |
| 300 | |a 1 Online-Ressource (XII, 344 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology, Methods and Protocols |v 725 | |
| 520 | |a With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Protein Science | |
| 650 | 4 | |a Genetic Engineering | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Genetic engineering | |
| 650 | 4 | |a Proteins | |
| 700 | 1 | |a Hobman, Tom C. |4 edt | |
| 700 | 1 | |a Duchaine, Thomas F. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781617790454 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-61779-046-1 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344097 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-61779-046-1 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-61779-046-1 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Record in the Search Index
| _version_ | 1804178539833982976 |
|---|---|
| any_adam_object | |
| author2 | Hobman, Tom C. Duchaine, Thomas F. |
| author2_role | edt edt |
| author2_variant | t c h tc tch t f d tf tfd |
| author_facet | Hobman, Tom C. Duchaine, Thomas F. |
| building | Verbundindex |
| bvnumber | BV044951340 |
| classification_rvk | WD 5355 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-61779-046-1 (OCoLC)891109516 (DE-599)BVBBV044951340 |
| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-61779-046-1 |
| edition | 1 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02865nmm a2200481zcb4500</leader><controlfield tag="001">BV044951340</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2011 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781617790461</subfield><subfield code="9">978-1-61779-046-1</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-61779-046-1</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-61779-046-1</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)891109516</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951340</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5355</subfield><subfield code="0">(DE-625)148201:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Argonaute Proteins</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Tom C. Hobman, Thomas F. Duchaine</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XII, 344 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology, Methods and Protocols</subfield><subfield code="v">725</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Protein Science</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetic Engineering</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetic engineering</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hobman, Tom C.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Duchaine, Thomas F.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781617790454</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-61779-046-1</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344097</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-61779-046-1</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-61779-046-1</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044951340 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781617790461 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344097 |
| oclc_num | 891109516 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XII, 344 p) |
| psigel | ZDB-2-PRO |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology, Methods and Protocols |
| spelling | Argonaute Proteins Methods and Protocols edited by Tom C. Hobman, Thomas F. Duchaine 1 Totowa, NJ Humana Press 2011 1 Online-Ressource (XII, 344 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 725 With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins Hobman, Tom C. edt Duchaine, Thomas F. edt Erscheint auch als Druck-Ausgabe 9781617790454 https://doi.org/10.1007/978-1-61779-046-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Argonaute Proteins Methods and Protocols Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins |
| title | Argonaute Proteins Methods and Protocols |
| title_auth | Argonaute Proteins Methods and Protocols |
| title_exact_search | Argonaute Proteins Methods and Protocols |
| title_full | Argonaute Proteins Methods and Protocols edited by Tom C. Hobman, Thomas F. Duchaine |
| title_fullStr | Argonaute Proteins Methods and Protocols edited by Tom C. Hobman, Thomas F. Duchaine |
| title_full_unstemmed | Argonaute Proteins Methods and Protocols edited by Tom C. Hobman, Thomas F. Duchaine |
| title_short | Argonaute Proteins |
| title_sort | argonaute proteins methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins |
| topic_facet | Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins |
| url | https://doi.org/10.1007/978-1-61779-046-1 |
| work_keys_str_mv | AT hobmantomc argonauteproteinsmethodsandprotocols AT duchainethomasf argonauteproteinsmethodsandprotocols |