Transmembrane Signaling Protocols:
The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded s...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
332 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today |
| Beschreibung: | 1 Online-Ressource (XII, 364 p. 54 illus., 1 illus. in color) |
| ISBN: | 9781597450485 |
| DOI: | 10.1385/1597450480 |
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| 520 | |a The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today | ||
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Datensatz im Suchindex
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| spelling | Transmembrane Signaling Protocols edited by Hydar Ali, Bodduluri Haribabu Second Edition Totowa, NJ Humana Press 2006 1 Online-Ressource (XII, 364 p. 54 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 332 The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Plasmamembran (DE-588)4067550-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Signaltransduktion (DE-588)4318717-1 s Plasmamembran (DE-588)4067550-6 s Labortechnik (DE-588)4123602-6 s b DE-604 Ali, Hydar edt Haribabu, Bodduluri edt Erscheint auch als Druck-Ausgabe 9781588295460 https://doi.org/10.1385/1597450480 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Transmembrane Signaling Protocols Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd Plasmamembran (DE-588)4067550-6 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4318717-1 (DE-588)4067550-6 (DE-588)4143413-4 |
| title | Transmembrane Signaling Protocols |
| title_auth | Transmembrane Signaling Protocols |
| title_exact_search | Transmembrane Signaling Protocols |
| title_full | Transmembrane Signaling Protocols edited by Hydar Ali, Bodduluri Haribabu |
| title_fullStr | Transmembrane Signaling Protocols edited by Hydar Ali, Bodduluri Haribabu |
| title_full_unstemmed | Transmembrane Signaling Protocols edited by Hydar Ali, Bodduluri Haribabu |
| title_short | Transmembrane Signaling Protocols |
| title_sort | transmembrane signaling protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd Plasmamembran (DE-588)4067550-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Labortechnik Signaltransduktion Plasmamembran Aufsatzsammlung |
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