Cell Cycle Synchronization: Methods and Protocols
To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure...
Gespeichert in:
Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2011
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Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
761 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the "arrest-and-release" approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so |
Beschreibung: | 1 Online-Ressource (XII, 300 p. 57 illus) |
ISBN: | 9781617791826 |
DOI: | 10.1007/978-1-61779-182-6 |
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Datensatz im Suchindex
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author2 | Banfalvi, Gaspar |
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discipline | Biologie |
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spelling | Cell Cycle Synchronization Methods and Protocols edited by Gaspar Banfalvi Totowa, NJ Humana Press 2011 1 Online-Ressource (XII, 300 p. 57 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 761 To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the "arrest-and-release" approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so Life Sciences Cell Biology Cell Cycle Analysis Life sciences Cell biology Cell cycle Banfalvi, Gaspar edt Erscheint auch als Druck-Ausgabe 9781617791819 https://doi.org/10.1007/978-1-61779-182-6 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Cell Cycle Synchronization Methods and Protocols Life Sciences Cell Biology Cell Cycle Analysis Life sciences Cell biology Cell cycle |
title | Cell Cycle Synchronization Methods and Protocols |
title_auth | Cell Cycle Synchronization Methods and Protocols |
title_exact_search | Cell Cycle Synchronization Methods and Protocols |
title_full | Cell Cycle Synchronization Methods and Protocols edited by Gaspar Banfalvi |
title_fullStr | Cell Cycle Synchronization Methods and Protocols edited by Gaspar Banfalvi |
title_full_unstemmed | Cell Cycle Synchronization Methods and Protocols edited by Gaspar Banfalvi |
title_short | Cell Cycle Synchronization |
title_sort | cell cycle synchronization methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Cell Biology Cell Cycle Analysis Life sciences Cell biology Cell cycle |
topic_facet | Life Sciences Cell Biology Cell Cycle Analysis Life sciences Cell biology Cell cycle |
url | https://doi.org/10.1007/978-1-61779-182-6 |
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