Mass Spectrometry of Glycoproteins: Methods and Protocols

 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the...

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Bibliographic Details
Other Authors: Kohler, Jennifer J. (Editor), Patrie, Steven M. (Editor)
Format: Electronic eBook
Language:English
Published: Totowa, NJ Humana Press 2013
Series:Methods in Molecular Biology, Methods and Protocols 951
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Online Access:UBR01
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Summary: Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry
Physical Description:1 Online-Ressource (XI, 339 p. 90 illus., 12 illus. in color)
ISBN:9781627031462
DOI:10.1007/978-1-62703-146-2

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