Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond...

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Bibliographic Details

Other Authors:	Williams, Mark A. (Editor), Daviter, Tina (Editor)
Format:	Electronic eBook
Language:	English
Published:	Totowa, NJ Humana Press 2013
Edition:	2nd ed. 2013
Series:	Methods in Molecular Biology, Methods and Protocols 1008
Subjects:	Life Sciences Protein Science Protein-Ligand Interactions Life sciences Proteins Molekularbiologische Methode Ligand > Biochemie Wechselwirkung Proteine Aufsatzsammlung
Online Access:	UBR01 TUM01 Volltext
Summary:	Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology
Physical Description:	1 Online-Ressource (X, 530 p. 146 illus., 45 illus. in color)
ISBN:	9781627033985
DOI:	10.1007/978-1-62703-398-5