Cytochrome P450 protocols:
In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in Molecular Biology™
107 |
| Schlagworte: | |
| Online-Zugang: | UBR01 URL des Erstveröffentlichers |
| Zusammenfassung: | In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques. Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants |
| Beschreibung: | 1 Online-Ressource (XIV, 482 p. 49 illus) |
| ISBN: | 9781592595808 |
| DOI: | 10.1385/0896035190 |
Internformat
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Datensatz im Suchindex
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| any_adam_object | |
| author2 | Phillips, Ian R. Shephard, Elizabeth A. |
| author2_role | edt edt |
| author2_variant | i r p ir irp e a s ea eas |
| author_facet | Phillips, Ian R. Shephard, Elizabeth A. |
| building | Verbundindex |
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| collection | ZDB-2-PRO |
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| dewey-full | 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572 |
| dewey-search | 572 |
| dewey-sort | 3572 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/0896035190 |
| format | Electronic eBook |
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| isbn | 9781592595808 |
| language | English |
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| spelling | Cytochrome P450 protocols edited by Ian R. Phillips, Elizabeth A. Shephard Totowa, NJ Humana Press 1998 1 Online-Ressource (XIV, 482 p. 49 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 107 In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques. Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants Life Sciences Biochemistry, general Life sciences Biochemistry Methode (DE-588)4038971-6 gnd rswk-swf Cytochrom P-450 (DE-588)4148705-9 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Cytochrom P-450 (DE-588)4148705-9 s Methode (DE-588)4038971-6 s DE-604 Molekularbiologie (DE-588)4039983-7 s Biochemische Methode (DE-588)4319880-6 s Phillips, Ian R. edt Shephard, Elizabeth A. edt Erscheint auch als Druck-Ausgabe 978-0-89603-519-5 https://doi.org/10.1385/0896035190 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Cytochrome P450 protocols Life Sciences Biochemistry, general Life sciences Biochemistry Methode (DE-588)4038971-6 gnd Cytochrom P-450 (DE-588)4148705-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4148705-9 (DE-588)4039983-7 (DE-588)4319880-6 (DE-588)4143413-4 |
| title | Cytochrome P450 protocols |
| title_auth | Cytochrome P450 protocols |
| title_exact_search | Cytochrome P450 protocols |
| title_full | Cytochrome P450 protocols edited by Ian R. Phillips, Elizabeth A. Shephard |
| title_fullStr | Cytochrome P450 protocols edited by Ian R. Phillips, Elizabeth A. Shephard |
| title_full_unstemmed | Cytochrome P450 protocols edited by Ian R. Phillips, Elizabeth A. Shephard |
| title_short | Cytochrome P450 protocols |
| title_sort | cytochrome p450 protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Methode (DE-588)4038971-6 gnd Cytochrom P-450 (DE-588)4148705-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Methode Cytochrom P-450 Molekularbiologie Biochemische Methode Aufsatzsammlung |
| url | https://doi.org/10.1385/0896035190 |
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