RNA Silencing: Methods and Protocols
The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and d...
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| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2005
|
| Series: | Methods in Molecular Biology™
309 |
| Subjects: | |
| Online Access: | UBR01 TUM01 Volltext |
| Summary: | The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications |
| Physical Description: | 1 Online-Ressource (X, 338 p. 74 illus) |
| ISBN: | 9781592599356 |
| DOI: | 10.1385/1592599354 |
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| spelling | RNA Silencing Methods and Protocols edited by Gordon G. Carmichael Totowa, NJ Humana Press 2005 1 Online-Ressource (X, 338 p. 74 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 309 The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications Life Sciences Cell Biology Life sciences Cell biology Geninaktivierung (DE-588)4820638-6 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Geninaktivierung (DE-588)4820638-6 s DE-604 Genexpression (DE-588)4020136-3 s Genregulation (DE-588)4122166-7 s b DE-604 Carmichael, Gordon G. edt Erscheint auch als Druck-Ausgabe 9781588294364 https://doi.org/10.1385/1592599354 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | RNA Silencing Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Geninaktivierung (DE-588)4820638-6 gnd Genregulation (DE-588)4122166-7 gnd Genexpression (DE-588)4020136-3 gnd RNS (DE-588)4076759-0 gnd |
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| title | RNA Silencing Methods and Protocols |
| title_auth | RNA Silencing Methods and Protocols |
| title_exact_search | RNA Silencing Methods and Protocols |
| title_full | RNA Silencing Methods and Protocols edited by Gordon G. Carmichael |
| title_fullStr | RNA Silencing Methods and Protocols edited by Gordon G. Carmichael |
| title_full_unstemmed | RNA Silencing Methods and Protocols edited by Gordon G. Carmichael |
| title_short | RNA Silencing |
| title_sort | rna silencing methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Geninaktivierung (DE-588)4820638-6 gnd Genregulation (DE-588)4122166-7 gnd Genexpression (DE-588)4020136-3 gnd RNS (DE-588)4076759-0 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Geninaktivierung Genregulation Genexpression RNS Aufsatzsammlung |
| url | https://doi.org/10.1385/1592599354 |
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