Kinesin Protocols:
It is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most produ...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in Molecular Biology™
164 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | It is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Detailed examples of how to address functional studies are also presented, along with some very new methods for studying the role of KLP in the organization of microtubules in three dimensions. There are also advanced methods for the study of kinesins at the structural level. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed successfully to study the multifaceted world of kinesin-like proteins and to explore their many functions |
| Beschreibung: | 1 Online-Ressource (XI, 258 p) |
| ISBN: | 9781592590698 |
| DOI: | 10.1385/1592590691 |
Internformat
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| 520 | |a It is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Detailed examples of how to address functional studies are also presented, along with some very new methods for studying the role of KLP in the organization of microtubules in three dimensions. There are also advanced methods for the study of kinesins at the structural level. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed successfully to study the multifaceted world of kinesin-like proteins and to explore their many functions | ||
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| id | DE-604.BV044950991 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781592590698 |
| language | English |
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| spelling | Kinesin Protocols edited by Isabelle Vernos Totowa, NJ Humana Press 2001 1 Online-Ressource (XI, 258 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 164 It is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Detailed examples of how to address functional studies are also presented, along with some very new methods for studying the role of KLP in the organization of microtubules in three dimensions. There are also advanced methods for the study of kinesins at the structural level. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed successfully to study the multifaceted world of kinesin-like proteins and to explore their many functions Life Sciences Cell Biology Life sciences Cell biology Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Kinesin (DE-588)4236029-8 gnd rswk-swf Kinesin (DE-588)4236029-8 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Vernos, Isabelle edt Erscheint auch als Druck-Ausgabe 9780896037663 https://doi.org/10.1385/1592590691 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Kinesin Protocols Life Sciences Cell Biology Life sciences Cell biology Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Kinesin (DE-588)4236029-8 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 (DE-588)4236029-8 |
| title | Kinesin Protocols |
| title_auth | Kinesin Protocols |
| title_exact_search | Kinesin Protocols |
| title_full | Kinesin Protocols edited by Isabelle Vernos |
| title_fullStr | Kinesin Protocols edited by Isabelle Vernos |
| title_full_unstemmed | Kinesin Protocols edited by Isabelle Vernos |
| title_short | Kinesin Protocols |
| title_sort | kinesin protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Kinesin (DE-588)4236029-8 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Molekularbiologie Methode Kinesin |
| url | https://doi.org/10.1385/1592590691 |
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