Heterologous Expression of Membrane Proteins: Methods and Protocols
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins:...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2010
|
| Schriftenreihe: | Methods in Molecular Biology™, Methods and Protocols
601 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. |
| Beschreibung: | 1 Online-Ressource (XII, 272 p. 44 illus., 5 illus. in color) |
| ISBN: | 9781607613442 |
| DOI: | 10.1007/978-1-60761-344-2 |
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| spelling | Heterologous Expression of Membrane Proteins Methods and Protocols edited by Isabelle Mus-Veteau Totowa, NJ Humana Press 2010 1 Online-Ressource (XII, 272 p. 44 illus., 5 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™, Methods and Protocols 601 Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. Life Sciences Protein Science Biochemistry, general Cell Biology Proteomics Membrane Biology Life sciences Biochemistry Proteins Cell biology Cell membranes Heterologe Genexpression (DE-588)4342015-1 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Membranproteine (DE-588)4130026-9 s Heterologe Genexpression (DE-588)4342015-1 s DE-604 Mus-Veteau, Isabelle edt Erscheint auch als Druck-Ausgabe 9781607613435 https://doi.org/10.1007/978-1-60761-344-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Heterologous Expression of Membrane Proteins Methods and Protocols Life Sciences Protein Science Biochemistry, general Cell Biology Proteomics Membrane Biology Life sciences Biochemistry Proteins Cell biology Cell membranes Heterologe Genexpression (DE-588)4342015-1 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4342015-1 (DE-588)4130026-9 (DE-588)4143413-4 |
| title | Heterologous Expression of Membrane Proteins Methods and Protocols |
| title_auth | Heterologous Expression of Membrane Proteins Methods and Protocols |
| title_exact_search | Heterologous Expression of Membrane Proteins Methods and Protocols |
| title_full | Heterologous Expression of Membrane Proteins Methods and Protocols edited by Isabelle Mus-Veteau |
| title_fullStr | Heterologous Expression of Membrane Proteins Methods and Protocols edited by Isabelle Mus-Veteau |
| title_full_unstemmed | Heterologous Expression of Membrane Proteins Methods and Protocols edited by Isabelle Mus-Veteau |
| title_short | Heterologous Expression of Membrane Proteins |
| title_sort | heterologous expression of membrane proteins methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Biochemistry, general Cell Biology Proteomics Membrane Biology Life sciences Biochemistry Proteins Cell biology Cell membranes Heterologe Genexpression (DE-588)4342015-1 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Life Sciences Protein Science Biochemistry, general Cell Biology Proteomics Membrane Biology Life sciences Biochemistry Proteins Cell biology Cell membranes Heterologe Genexpression Membranproteine Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-60761-344-2 |
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