Recombinant Gene Expression Protocols:
The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse quent expansion is the cornerstone of modem molecular biology. A direct p...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1997
|
| Schriftenreihe: | Methods in Molecular Biology
62 |
| Schlagworte: | |
| Online-Zugang: | UBR01 URL des Erstveröffentlichers |
| Zusammenfassung: | The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmammalian eukaryotes such as plants, Xenopus, and insects, as well as in transgenic organisms. In-depth information is presented on the important characteristics of expression cloning vectors and the various methods for efficiently introducing expression constructs into target cells and/ or organisms. Throughout Recombinant Gene Expression Protocols, the authors have consistently striven for a balanced presentation of both background informa tion and actual laboratory details |
| Beschreibung: | 1 Online-Ressource (XX, 521 p) |
| ISBN: | 9781592595488 |
| DOI: | 10.1385/0896034801 |
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| 520 | |a The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmammalian eukaryotes such as plants, Xenopus, and insects, as well as in transgenic organisms. In-depth information is presented on the important characteristics of expression cloning vectors and the various methods for efficiently introducing expression constructs into target cells and/ or organisms. Throughout Recombinant Gene Expression Protocols, the authors have consistently striven for a balanced presentation of both background informa tion and actual laboratory details | ||
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Datensatz im Suchindex
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| spelling | Recombinant Gene Expression Protocols edited by Rocky S. Tuan Totowa, NJ Humana Press 1997 1 Online-Ressource (XX, 521 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 62 The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmammalian eukaryotes such as plants, Xenopus, and insects, as well as in transgenic organisms. In-depth information is presented on the important characteristics of expression cloning vectors and the various methods for efficiently introducing expression constructs into target cells and/ or organisms. Throughout Recombinant Gene Expression Protocols, the authors have consistently striven for a balanced presentation of both background informa tion and actual laboratory details Life Sciences Cell Biology Life sciences Cell biology Rekombinante DNS (DE-588)4225578-8 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Rekombinante DNS (DE-588)4225578-8 s Genexpression (DE-588)4020136-3 s DE-604 Methode (DE-588)4038971-6 s Tuan, Rocky S. edt Erscheint auch als Druck-Ausgabe 9780896034808 https://doi.org/10.1385/0896034801 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Recombinant Gene Expression Protocols Life Sciences Cell Biology Life sciences Cell biology Rekombinante DNS (DE-588)4225578-8 gnd Genexpression (DE-588)4020136-3 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4225578-8 (DE-588)4020136-3 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Recombinant Gene Expression Protocols |
| title_auth | Recombinant Gene Expression Protocols |
| title_exact_search | Recombinant Gene Expression Protocols |
| title_full | Recombinant Gene Expression Protocols edited by Rocky S. Tuan |
| title_fullStr | Recombinant Gene Expression Protocols edited by Rocky S. Tuan |
| title_full_unstemmed | Recombinant Gene Expression Protocols edited by Rocky S. Tuan |
| title_short | Recombinant Gene Expression Protocols |
| title_sort | recombinant gene expression protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Rekombinante DNS (DE-588)4225578-8 gnd Genexpression (DE-588)4020136-3 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Rekombinante DNS Genexpression Methode Aufsatzsammlung |
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