Transgenic Plants: Methods and Protocols:
Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneratio...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology™
286 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus. Also described are the use of selectable and reporter markers, positive selection (for maize transformation), and the controlled excision and removal of marker genes from both nuclei and plastids once transgenic shoots have been efficiently generated. Additional techniques employ quantitative real-time PCR, reverse transcription PCR, fluorescence in situ hybridization, and thermal asymmetric interlaced PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Transgenic Plants: Methods and Protocols offers both novice and experienced researchers a cutting-edge laboratory manual covering all aspects of plant genetic transformation and transgenic crops |
| Beschreibung: | 1 Online-Ressource (XIV, 437 p) |
| ISBN: | 9781592598274 |
| DOI: | 10.1385/1592598277 |
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| 520 | |a Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus. Also described are the use of selectable and reporter markers, positive selection (for maize transformation), and the controlled excision and removal of marker genes from both nuclei and plastids once transgenic shoots have been efficiently generated. Additional techniques employ quantitative real-time PCR, reverse transcription PCR, fluorescence in situ hybridization, and thermal asymmetric interlaced PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Transgenic Plants: Methods and Protocols offers both novice and experienced researchers a cutting-edge laboratory manual covering all aspects of plant genetic transformation and transgenic crops | ||
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Datensatz im Suchindex
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| any_adam_object | |
| author2 | Peña, Leandro |
| author2_role | edt |
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| building | Verbundindex |
| bvnumber | BV044950606 |
| callnumber-first | S - Agriculture |
| callnumber-label | SB123 |
| callnumber-raw | SB123.57 |
| callnumber-search | SB123.57 |
| callnumber-sort | SB 3123.57 |
| callnumber-subject | SB - Plant Culture |
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| ctrlnum | (ZDB-2-PRO)978-1-59259-827-4 (OCoLC)730584245 (DE-599)BVBBV044950606 |
| dewey-full | 631.5/233 580 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 631 - Techniques, equipment & materials 580 - Plants |
| dewey-raw | 631.5/233 580 |
| dewey-search | 631.5/233 580 |
| dewey-sort | 3631.5 3233 |
| dewey-tens | 630 - Agriculture and related technologies 580 - Plants |
| discipline | Biologie Agrarwissenschaft Chemie-Ingenieurwesen Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Biotechnologie Pflanzenbau |
| doi_str_mv | 10.1385/1592598277 |
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| spelling | Transgenic Plants: Methods and Protocols edited by Leandro Peña Totowa, NJ Humana Press 2004 1 Online-Ressource (XIV, 437 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 286 Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus. Also described are the use of selectable and reporter markers, positive selection (for maize transformation), and the controlled excision and removal of marker genes from both nuclei and plastids once transgenic shoots have been efficiently generated. Additional techniques employ quantitative real-time PCR, reverse transcription PCR, fluorescence in situ hybridization, and thermal asymmetric interlaced PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Transgenic Plants: Methods and Protocols offers both novice and experienced researchers a cutting-edge laboratory manual covering all aspects of plant genetic transformation and transgenic crops Life Sciences Plant Sciences Transgenics Life sciences Plant science Botany Transgenic organisms Labortechnik (DE-588)4123602-6 gnd rswk-swf Transgene Pflanzen (DE-588)4311513-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Transgene Pflanzen (DE-588)4311513-5 s Labortechnik (DE-588)4123602-6 s b DE-604 Peña, Leandro edt Erscheint auch als Druck-Ausgabe 9781588292636 https://doi.org/10.1385/1592598277 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Transgenic Plants: Methods and Protocols Life Sciences Plant Sciences Transgenics Life sciences Plant science Botany Transgenic organisms Labortechnik (DE-588)4123602-6 gnd Transgene Pflanzen (DE-588)4311513-5 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4311513-5 (DE-588)4143413-4 |
| title | Transgenic Plants: Methods and Protocols |
| title_auth | Transgenic Plants: Methods and Protocols |
| title_exact_search | Transgenic Plants: Methods and Protocols |
| title_full | Transgenic Plants: Methods and Protocols edited by Leandro Peña |
| title_fullStr | Transgenic Plants: Methods and Protocols edited by Leandro Peña |
| title_full_unstemmed | Transgenic Plants: Methods and Protocols edited by Leandro Peña |
| title_short | Transgenic Plants: Methods and Protocols |
| title_sort | transgenic plants methods and protocols |
| topic | Life Sciences Plant Sciences Transgenics Life sciences Plant science Botany Transgenic organisms Labortechnik (DE-588)4123602-6 gnd Transgene Pflanzen (DE-588)4311513-5 gnd |
| topic_facet | Life Sciences Plant Sciences Transgenics Life sciences Plant science Botany Transgenic organisms Labortechnik Transgene Pflanzen Aufsatzsammlung |
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