Artificial Riboswitches: Methods and Protocols
Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswi...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2014
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1111 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field |
| Beschreibung: | 1 Online-Ressource (X, 289 p. 75 illus., 45 illus. in color) |
| ISBN: | 9781627037556 |
| DOI: | 10.1007/978-1-62703-755-6 |
Internformat
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Datensatz im Suchindex
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| dewey-full | 572.88 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.88 572 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-62703-755-6 |
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| spelling | Artificial Riboswitches Methods and Protocols edited by Atsushi Ogawa Totowa, NJ Humana Press 2014 1 Online-Ressource (X, 289 p. 75 illus., 45 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1111 Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field Life Sciences Biochemistry, general Biochemical Engineering Life sciences Biochemical engineering Biochemistry Labortechnik (DE-588)4123602-6 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Genregulation (DE-588)4122166-7 s Labortechnik (DE-588)4123602-6 s DE-604 Ogawa, Atsushi edt Erscheint auch als Druck-Ausgabe 9781627037549 https://doi.org/10.1007/978-1-62703-755-6 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Artificial Riboswitches Methods and Protocols Life Sciences Biochemistry, general Biochemical Engineering Life sciences Biochemical engineering Biochemistry Labortechnik (DE-588)4123602-6 gnd Genregulation (DE-588)4122166-7 gnd RNS (DE-588)4076759-0 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4122166-7 (DE-588)4076759-0 (DE-588)4143413-4 |
| title | Artificial Riboswitches Methods and Protocols |
| title_auth | Artificial Riboswitches Methods and Protocols |
| title_exact_search | Artificial Riboswitches Methods and Protocols |
| title_full | Artificial Riboswitches Methods and Protocols edited by Atsushi Ogawa |
| title_fullStr | Artificial Riboswitches Methods and Protocols edited by Atsushi Ogawa |
| title_full_unstemmed | Artificial Riboswitches Methods and Protocols edited by Atsushi Ogawa |
| title_short | Artificial Riboswitches |
| title_sort | artificial riboswitches methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Biochemical Engineering Life sciences Biochemical engineering Biochemistry Labortechnik (DE-588)4123602-6 gnd Genregulation (DE-588)4122166-7 gnd RNS (DE-588)4076759-0 gnd |
| topic_facet | Life Sciences Biochemistry, general Biochemical Engineering Life sciences Biochemical engineering Biochemistry Labortechnik Genregulation RNS Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-62703-755-6 |
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