SUMO: Methods and Protocols
This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of t...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2016
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| Schriftenreihe: | Methods in Molecular Biology
1475 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls. Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system |
| Beschreibung: | 1 Online-Ressource (XI, 306 p. 54 illus., 34 illus. in color) |
| ISBN: | 9781493963584 |
| DOI: | 10.1007/978-1-4939-6358-4 |
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| language | English |
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| spelling | SUMO Methods and Protocols edited by Manuel S. Rodriguez New York, NY Springer New York 2016 1 Online-Ressource (XI, 306 p. 54 illus., 34 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1475 This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls. Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system Life Sciences Biochemistry, general Life sciences Biochemistry Rodriguez, Manuel S. edt Erscheint auch als Druck-Ausgabe 9781493963560 https://doi.org/10.1007/978-1-4939-6358-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | SUMO Methods and Protocols Life Sciences Biochemistry, general Life sciences Biochemistry |
| title | SUMO Methods and Protocols |
| title_auth | SUMO Methods and Protocols |
| title_exact_search | SUMO Methods and Protocols |
| title_full | SUMO Methods and Protocols edited by Manuel S. Rodriguez |
| title_fullStr | SUMO Methods and Protocols edited by Manuel S. Rodriguez |
| title_full_unstemmed | SUMO Methods and Protocols edited by Manuel S. Rodriguez |
| title_short | SUMO |
| title_sort | sumo methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry |
| url | https://doi.org/10.1007/978-1-4939-6358-4 |
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