Non-Viral Gene Delivery Vectors: Methods and Protocols
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors th...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2016
|
| Schriftenreihe: | Methods in Molecular Biology
1445 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field |
| Beschreibung: | 1 Online-Ressource (XIV, 290 p. 66 illus., 36 illus. in color) |
| ISBN: | 9781493937189 |
| DOI: | 10.1007/978-1-4939-3718-9 |
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| spelling | Non-Viral Gene Delivery Vectors Methods and Protocols edited by Gabriele Candiani New York, NY Springer New York 2016 1 Online-Ressource (XIV, 290 p. 66 illus., 36 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1445 This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field Biomedicine Human Genetics Medicine Human genetics Candiani, Gabriele edt Erscheint auch als Druck-Ausgabe 9781493937165 https://doi.org/10.1007/978-1-4939-3718-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Non-Viral Gene Delivery Vectors Methods and Protocols Biomedicine Human Genetics Medicine Human genetics |
| title | Non-Viral Gene Delivery Vectors Methods and Protocols |
| title_auth | Non-Viral Gene Delivery Vectors Methods and Protocols |
| title_exact_search | Non-Viral Gene Delivery Vectors Methods and Protocols |
| title_full | Non-Viral Gene Delivery Vectors Methods and Protocols edited by Gabriele Candiani |
| title_fullStr | Non-Viral Gene Delivery Vectors Methods and Protocols edited by Gabriele Candiani |
| title_full_unstemmed | Non-Viral Gene Delivery Vectors Methods and Protocols edited by Gabriele Candiani |
| title_short | Non-Viral Gene Delivery Vectors |
| title_sort | non viral gene delivery vectors methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-4939-3718-9 |
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