Morpholino Oligomers: Methods and Protocols
This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to struc...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1565 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier |
| Beschreibung: | 1 Online-Ressource (X, 284 p. 60 illus., 38 illus. in color) |
| ISBN: | 9781493968176 |
| DOI: | 10.1007/978-1-4939-6817-6 |
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| indexdate | 2024-07-10T08:05:33Z |
| institution | BVB |
| isbn | 9781493968176 |
| language | English |
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| spelling | Morpholino Oligomers Methods and Protocols edited by Hong M. Moulton, Jon D. Moulton New York, NY Springer New York 2017 1 Online-Ressource (X, 284 p. 60 illus., 38 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1565 This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier Life Sciences Biochemistry, general Life sciences Biochemistry Moulton, Hong M. edt Moulton, Jon D. edt Erscheint auch als Druck-Ausgabe 9781493968152 https://doi.org/10.1007/978-1-4939-6817-6 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Morpholino Oligomers Methods and Protocols Life Sciences Biochemistry, general Life sciences Biochemistry |
| title | Morpholino Oligomers Methods and Protocols |
| title_auth | Morpholino Oligomers Methods and Protocols |
| title_exact_search | Morpholino Oligomers Methods and Protocols |
| title_full | Morpholino Oligomers Methods and Protocols edited by Hong M. Moulton, Jon D. Moulton |
| title_fullStr | Morpholino Oligomers Methods and Protocols edited by Hong M. Moulton, Jon D. Moulton |
| title_full_unstemmed | Morpholino Oligomers Methods and Protocols edited by Hong M. Moulton, Jon D. Moulton |
| title_short | Morpholino Oligomers |
| title_sort | morpholino oligomers methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry |
| url | https://doi.org/10.1007/978-1-4939-6817-6 |
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