Drosophila cells in culture:
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
London ; San Diego, CA ; Cambridge, MA ; Oxford
Academic Press
[2018]
|
| Edition: | Second edition |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | xxii, 235 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128094730 |
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| 100 | 1 | |a Echalier, Guy |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Drosophila cells in culture |c Guy Echalier (Center National de la Recherche Scientifique, Universite Pierre et Marie Curie, Paris, France), Norbert Perrimon (Departement of Genetics, Harvard Medical School, Boston, MA, United States), Stephanie E. Mohr (Departement of Genetics, Harvard Medical School, Boston, MA, United States) |
| 250 | |a Second edition | ||
| 264 | 1 | |a London ; San Diego, CA ; Cambridge, MA ; Oxford |b Academic Press |c [2018] | |
| 300 | |a xxii, 235 Seiten |b Illustrationen, Diagramme | ||
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| 700 | 1 | |a Perrimon, Norbert |e Verfasser |4 aut | |
| 700 | 1 | |a Mohr, Stephanie Elizabeth |d 1971- |e Verfasser |0 (DE-588)1151833282 |4 aut | |
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Record in the Search Index
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| adam_text | Contents
Introduction to the Second Edition xv
General Introduction xvii
Part I
Historical Perspectives and Classical Methods
1. Composition of the Body Fluid of Drosophila
and the Design of Culture Media for Drosophila Cells
Guy Echafier
Introduction 4
Milieu Interieur of Drosophila Larva 4
L Biochemical Data About the Milieu Interieur of Drosophila 5
A. Osmotic Pressure 5
B. pH 6
C. Inorganic Ions 7
D. Amino Acid Pool 9
E. Organic Acid 13
F. Sugars 13
II. Main Available Culture Media for Drosophila Cells 13
A. Echalier and Ohanessian s D22 Medium 14
B. Gvozdev and Kakpakov s C-1 5 or S-15 Medium 16
C. Schneider s Medium 18
D. Medium M3: Shields and Sang s (1977)
+ Cross and Sang (1 978) 20
E. Wyss s Medium ZW 22
F. Robb s Medium R-14 25
III. General Discussion About the Characteristics and Main
Components of Culture Media for Tissues or
Cells of Drosophila 28
A. Osmolarity 29
B. pH and Buffers 30
C. Major Ions 30
D. Amino Acids 32
E. Sugars 34
F. Vitamins—Yeast Extracts 35
G. Serum Supplementation 37
vii
38
39
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40
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44
45
47
48
48
49
50
51
52
52
52
52
52
53
58
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61
62
62
62
63
63
63
63
64
64
64
64
64
Contents
H. Hormones and Growth Factors
I. Drosophila Fly and Egg Extracts
J. Antibiotics
IV Prospective Conclusions
References
Appendix 1.A (as relates to Table 1.5)
Appendix 1.B Stock Solution of Grace s Vitamins
Primary Cell Cultures of Drosophila Embryonic Cells
Guy Echalier
Introduction
I. Laboratory for Cell Culture
A. Setting up Embryonic Ceil Culture
B. Egg Collection
C. Surface Sterilization and Dechorination of the Eggs
D. Mechanical Egg Disruption and Dissociation of the Cell
IL Genera! Maintenance of These Primary Cultures
A. Temperature
B. Buffering
C. Routine Handling
111. Usual Sequence in Primary Cultures of Embryonic Cells
A. Early Evolution
B. Very Late Multiplications and Migrations (as a Prelude
to the Establishment In Vitro of a New Cell Line)
C. Important Note
References
Continuous Drosophila Cell Lines Established In Vitro
Guy Echalier
Introduction
Catalog of Some Interesting Cell Lines
I. Wild-Type Embryonic Cell Lines
A. Echalier and Ohanessian s (1969), Ohanessian and
Echalier (1968) Kc Line
B. Gvozdev and Kakpakov (1970),
Kakpakov et ak (1969) Dm Line
C. Schneider s Cell Lines (1972, 1971) SI, S2, S3
II. Embryonic Cell Lines From Mutants
A. Haploid Cell Lines
B. Acentriolar Cell Lines—Debec and Abbadie (1989),
Lecland et al. (2013)
C. Tumorous Cell Lines
ML Other Relevant Cell Lines
A. Cell Lines From Larva! or Adult Tissues
B. Cell Lines From Other Drosophila Species
Contents ix
IV. Maintenance of Drosophila Cell Lines 65
A. Routine Maintenance (as Monolayers) 65
V. Subcultures 66
A. Cell Cloning 67
B. Suspension Cultures 70
C. Storage by Freezing 72
VI. Karyology 73
A. Karyotype Analysis of Drosophila melanogaster 74
B. Karyological Techniques 75
C. Evolution of the Karyotype With Time 78
References 79
4. Cells or Tissues in Course of Differentiation
Guy Echalier
Introduction 82
I. Neurons 82
A. Neuroblasts and Neurons in Primary Embryonic
Cell Cultures 82
B. Asymmetric Neuroblast Divisions Generate Neural
Ganglion Cells (Seecof et al., 1973b;
Furst and Mahowald, 1 985b) 84
C. Sequence of Morphological Differentiation
of Neurons: Cone and Axon(s) Growth 84
D. Confirmations of the Authentic Neural Nature of These
In Vitro Differentiated Cells 86
II. Neuromuscular Junctions in Embryonic Cell Cultures 88
III. Cultures of Larval or Imaginal Neurons 89
A. Techniques for Dissociating Neurons: From the Larval
Central Nervous System (Wu et al., 1983b) 89
B. Evolution of Primary Culture of Dissociated Cells From
Drosophila Larval CNS (Wu et a!., 1 983b) 91
C. A Few Examples of the Possibilities Offered by
This Experimental Model (Most Publications Also
Came From Dr. Wu s Lab) 91
IV. Imaginal Discs 93
A. In Vivo Cultures of Drosophila Imaginal Discs 93
B. In Vitro Organ Cultures of Imaginal Discs 94
C. Continuous Cell Lines Derived From Cultures of
imaginal Discs (and Neural Tissues) 98
V. Conclusion and Perspectives 99
A. Improvements in the Composition of Culture Media 99
B. More Precise Choice of the Age of Material to be Cultured 99
C. Fiormonal Stimulations 99
D. Choice of Mutant Drosophila Strains 99
E. Alternative Succession of In Vivo and In Vitro Cultures 100
References 100
x Contents
5. Hormonal Control of Development of Insects:
Implications in Organ or Cell Cultures of Drosophila
Guy Echalier
Introduction 103
I. Ecdysteroids 105
A. Chemical Identification 105
B. Secretion During Drosophila Life 105
C. Nuclear Receptor 106
D. Other Active Steroids 106
E. Action of Ecdysteroids on Established Lines of Drosophila
Embryonic Cells (Essentially Kc Line) 106
F. Action of Ecdysteroids in Organ Cultures 119
II. Juvenile Hormone 119
A. JH Site of Synthesis 119
B. The Multiple JH Homologs 119
C. JH Assays 120
D. Release in Hemolymph 120
111 Insulin and Insulin-Like Factors 120
A. Pioneering Insulin Tests in Insect Cell Cultures 121
B. Different Insulin-Like Factors 122
C. Insulin Hormone Receptors 122
D. Physiological Functions 122
IV. Adipokinetic Hormone 123
A. Drosophila AKH 124
B. Corpora Cardiaca 124
C. AKH Mutants 124
V. Hormonal Interactions 124
References 125
Part II
Contemporary Approaches
6. Directed Genetic Methods for Establishing
Continuous Cell Lines
Amanda Simcox
Introduction 135
I. Expression of Activated Ras Rapidly and Reliably Produces
Continuous Cell Lines 136
II. Reduction of Expression of the PTEN or Hippo Pathway
Tumor Supressors Promotes Cell Line Generation 138
III. Using Ras Expression to Derive Cell Lines of Desired
Genotypes 139
IV. Potential for Ras Expression to Generate Cell
Type-Specific Cell Lines 139
V. What Lies Ahead 140
Contents xi
VI. Protocol for Establishing New Cell Lines Using the Rasv12
Approach 141
A. Establishing Rasv 12-Expressing Primary Cultures 141
B. Passaging Rasv1^Expressing Cell Cultures 143
References 143
7. Organ Culture Methods for the Drosophila Wing
Imaginai Disc
Jeremiah J. Zartman
I. Introduction 145
IL Summary of Recent Efforts 147
III. Organ Culture Methods 151
A. Basal Media Selection 151
B. Media Supplements 151
C. Preparation of Fly Extract 1 54
D. Preparation of Organ Culture Media 155
E. Wing Disc Dissection 156
F. Culture Chamber 157
IV. Additional Considerations 158
A. Batch Organ Cultures 158
B. Live Imaging of Organ Cultures 158
C. Comparison with in vivo Imaging Studies 158
V. Biochemical Assays to Characterize Organ Cultures 158
VI. Analysis of Live-Imaging Data 159
VIL Prospects and Open Questions 160
Notes on ZB Media 161
References 161
8. Functional Genomics Screens in Drosophila Cells
Stephanie E. Mohr and Norbert Perrimon
Introduction 165
I. Approaches to Screening 1 66
A. Screen Formats 166
B. Types of Screen Reagents 167
C Drosophila Cells as a Biosensor Screening Platform 1 68
D. Combining Perturbations (e.g., Double RNAi,
CRISPR + RNAi, drug + RNAi) 169
E. Relationship of Drosophila Cell Screens to Mammalian
Cell Screens and Systems 171
F. Cell Line Selection 172
II. Screen Assays 1 73
A. Miniaturization and Automation of Arrayed Assays 173
B. Cell Viability Assays 174
xil Contents
C. Transcriptional Reporter Assays 175
D. I mage-Based Assays 176
III. Screen Data Management and Analysis 176
A. Data Management 176
B. Monitoring Data During a Screen 177
C. Data Analysis 178
IV. Protocols for Cell-Based RNAi 180
A. dsRNA Synthesis 180
B. Cell-Based RNAi in a 6-Well Microwell Dish 183
C. Cell-Based RNAi in 384-Well Microwell Plates 184
D. Paraformaldehyde Fixation in 384-Well Assay Plates for
High-Content Image-Based Screens of Fixed Cultured or
Primary Cells 187
References 187
9, CRISPR-Based Perturbation of Gene Function
in Drosophila Cells
Benjamin E. Housden, Benjamin Ewen-Campen,
Stephanie E. Mohr■ and Norbert Perrimon
Introduction 193
I. Comparison of Approaches to Gene Perturbation 196
A. Comparing CRISPR Knockout to RNAi 1 96
B. ORF Overexpression vs CRISPRa 197
C. Use of CRISPR Technologies for Pooled Format Screens 198
D. General Limitations of Gene Disruption Technologies 198
II. Crispr Inactivation and Activation in Drosophila Cells 199
A. CRISPRi 199
B. CRISPRa 199
III. Methods to Assess Gene Perturbations 200
IV. Plasmid Vectors for CRISPR Technologies 200
V. Step-by-Step Protocols for Use of CRISPR Technologies
in Drosophila Cells 202
A. CRISPR sgRNA Design 202
B. CRISPR Knockout or Knock-in in Drosophila Cells 202
C. Single-Cell Cloning 202
D. CRISPRa in Drosophila Cells 203
References 204
10. Compendium of Drosophila Cell Line Resources
and Plasmid Vectors at the Drosophila Genome
Resource Center
Lei Gong, Kris Kfueg, Lucy Cherbas/ and Andrew C. Zelhof
Introduction 207
Contents xiii
I. The Cultured Cell Line Collection at the DGRC 208
A. Overview of Cell Lines 208
B. Specific Information for the Most Requested Cell Lines 209
II. Transcriptome Analysis of Drosophila Cell Lines 212
III. Plasmid Vectors Useful for Cell Culture Work 213
A. Plasmid Vectors Useful for Building Constructs
for Transient Transfection 213
B. Plasmids and Cells for Generation of Stable Transformed
Ceil Lines 21 3
C. Tagged Open Reading Frame (ORF) Collections
for Expression in Drosophila Cultured Cells 214
D. Extracellular Domain (ECD) Collection 215
E. Constructs for Tagging of Transfected Cells for Live-Cell
Flow Cytometry 215
IV. Protocols 215
A. General Considerations (See Also Chapter 3, Section 33V) 216
B. Culture Media and Supplements 216
C. Thawing and Freezing Cells 219
D. Additional Protocols 221
References 224
Index 227
|
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| indexdate | 2024-07-10T08:00:21Z |
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| language | English |
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| spelling | Echalier, Guy Verfasser aut Drosophila cells in culture Guy Echalier (Center National de la Recherche Scientifique, Universite Pierre et Marie Curie, Paris, France), Norbert Perrimon (Departement of Genetics, Harvard Medical School, Boston, MA, United States), Stephanie E. Mohr (Departement of Genetics, Harvard Medical School, Boston, MA, United States) Second edition London ; San Diego, CA ; Cambridge, MA ; Oxford Academic Press [2018] xxii, 235 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index Zellkultur (DE-588)4067547-6 gnd rswk-swf Drosophila (DE-588)4150708-3 gnd rswk-swf Drosophila (DE-588)4150708-3 s Zellkultur (DE-588)4067547-6 s DE-604 Perrimon, Norbert Verfasser aut Mohr, Stephanie Elizabeth 1971- Verfasser (DE-588)1151833282 aut Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030118376&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Echalier, Guy Perrimon, Norbert Mohr, Stephanie Elizabeth 1971- Drosophila cells in culture Zellkultur (DE-588)4067547-6 gnd Drosophila (DE-588)4150708-3 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4150708-3 |
| title | Drosophila cells in culture |
| title_auth | Drosophila cells in culture |
| title_exact_search | Drosophila cells in culture |
| title_full | Drosophila cells in culture Guy Echalier (Center National de la Recherche Scientifique, Universite Pierre et Marie Curie, Paris, France), Norbert Perrimon (Departement of Genetics, Harvard Medical School, Boston, MA, United States), Stephanie E. Mohr (Departement of Genetics, Harvard Medical School, Boston, MA, United States) |
| title_fullStr | Drosophila cells in culture Guy Echalier (Center National de la Recherche Scientifique, Universite Pierre et Marie Curie, Paris, France), Norbert Perrimon (Departement of Genetics, Harvard Medical School, Boston, MA, United States), Stephanie E. Mohr (Departement of Genetics, Harvard Medical School, Boston, MA, United States) |
| title_full_unstemmed | Drosophila cells in culture Guy Echalier (Center National de la Recherche Scientifique, Universite Pierre et Marie Curie, Paris, France), Norbert Perrimon (Departement of Genetics, Harvard Medical School, Boston, MA, United States), Stephanie E. Mohr (Departement of Genetics, Harvard Medical School, Boston, MA, United States) |
| title_short | Drosophila cells in culture |
| title_sort | drosophila cells in culture |
| topic | Zellkultur (DE-588)4067547-6 gnd Drosophila (DE-588)4150708-3 gnd |
| topic_facet | Zellkultur Drosophila |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030118376&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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