Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major: = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Erlangen ; Nürnberg
2017
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 161 Seiten Illustrationen, Diagramme |
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| 245 | 1 | 0 | |a Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major |b = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |c vorgelegt von Sabine Leitherer aus Bamberg |
| 246 | 1 | 1 | |a Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |
| 264 | 1 | |a Erlangen ; Nürnberg |c 2017 | |
| 300 | |a 161 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2017 | ||
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Record in the Search Index
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| adam_text | TABLE OF CONTENT
Z U S AM M E N FA SS U N G
..............................................................................................................................................................................-1
S U M M ARY
..................................................................................................................................................................................................-
3
1 IN TRO D U C TIO N
..........................................................................................................................................................................................
- 5
1.1 LEISHMANIA DISEASE AND
PARASITE.........................................................................................................................
...............- 5
1.1.1 EPIDEMIOLOGY OF
LEISHMANIASIS..................................................................................................................................
- 5
1.1.1 LIFE CYCLE OF LEISHMANIA
PARASITES...........................................................................................................................-
8
1.2 SURFACE COAT OF LEISHMANIA AND MECHANISM OF PROTEIN
EXPORT..................................................................................-1
1
1.2.1 SURFACE
COAT...................................................................................................................................................................-1
1
1.2.2 SECRETORY PATHWAY IN LEISHM
ANIA...........................................................................................................................-
1 3
1.3 IMMUNE RESPONSE DURING LEISHMANIA
INFECTION.............................................................................................................
- 1 5
1.3.1 INNATE IMMUNE RESPONSE DURING LEISHMANIA
INFECTION...................................................................................
- 1 5
1.3.2 ADAPTIVE IMMUNE RESPONSE DURING LEISHMANIA INFECTION: HOST
RESISTANCE AND SUSCEPTIBILITY.............- IS
1.4 IMMUNE EVASION STRATEGIES OF LEISHMANIA
PARASITES...................................................................................................
- 1 9
1.4.1 INTERFERENCE WITH HOST CELL
SIGNALING......................................................................................................................-
1 9
1.4.2 VIRULENCE
FACTORS...........................................................................................................................................................-2
1
1.5 SEARCH FOR PUTATIVE VIRULENCE FACTORS OF LEISHMANIA: DESIGN OF THE
PROJECT
..........................................................
- 23
1.5.1 PHOSPHATASES AS VIRULENCE FACTORS IN LEISHM
ANIA............................................................................................-
2 3
1.5.2 THE PHOSPHATASES OF REGENERATING L
IVER............................................................................................................-
2 6
2 S CIENTIFIC QUESTION, W O RKIN G HYPOTHESIS AND A IM S
...........................................................................................................-
29
3 M ATERIAL AND M E TH O D S
.....................................................................................................................................................................
- 31
3.1 M
ATERIAL......................................................................................................................................................................................
-3 1
3.2 MOLECULAR BIOLOGY
METHODS...................................................................................................................................................-
39
3.3 BIOCHEMISTRY
METHODS...........................................................................................................................................................
- 4 7
3.4 CELL CULTURE M
ETHODS..............................................................................................................................................................-
51
3.5 FLUORESCENCE MICROSCOPY AND F A C S
...............................................................................................................................-
59
3.6 ANIMAL
EXPERIMENTS................................................................................................................................................................
- 61
3.7 STATISTICAL
ANALYSIS...................................................................................................................................................................-
64
4 R E S U LTS
..................................................................................................................................................................................................-
65
4.1 STRUCTURE AND BIOCHEMICAL CHARACTERIZATION OF LM P R L
...............................................................................................
- 6 5
4.1.1 ANALYSIS OF THE SEQUENCE AND STRUCTURE OF LMPRL PHOSPHATASES
..............................................................
- 6 5
4.1.2 BIOCHEMICAL CHARACTERIZATION OF THE LMPRL PHOSPHATASE
ACTIVITY................................................................-
69
4.2 FUNCTIONAL CHARACTERIZATION OF LMPRL IN L M AJOR
PARASITES.....................................................................................-
73
4.2.1 CHARACTERIZATION OF THE PROTEIN EXPRESSION PROFILE OF LMPRL IN L M
AJOR PARASITES................................- 73
4.2.2 INFLUENCE OF LMPRL ON THE REPLICATION OF L M AJOR
PROMASTIGOTES................................................................-
78
4 .2 .3 CHARACTERIZATION OF THE SUBCELLULAR LOCALIZATION OF LMPRL IN L M
AJOR PROMASTIGOTES
...........................
- 8 4 -
4 .2.4 CHARACTERIZATION OF THE SECRETION OF LMPRL BY L M AJOR
PROMASTIGOTES....................................................- 8 6 -
4 .3 FUNCTIONAL CHARACTERIZATION OF LMPRL DURING MACROPHAGE INFECTION IN
V ITRO
..........................................................
- 8 9 -
4.3.1 EXPRESSION OF LMPRL DURING IN VITRO INFECTION OF M
ACROPHAGES...................................................................-
89 -
4.3.2 SUBCELLULAR LOCALIZATION OF HAS-LMPRL DURING MACROPHAGE INFECTION
WITH L M AJOR PROMASTIGOTES ..-9 1 -
4 .3.3 INFLUENCE OF HA3-LMPRL OVER-EXPRESSION BY L. M AJOR DURING
INFECTION OF M ACROPHAGES.....................- 9 5 -
4 .3 .4 IMPACT OF LMPRL DELETION IN L M AJOR PARASITES DURING MACROPHAGE
INFECTION
.......................................
- 98 -
4.4 CHARACTERIZATION OF THE VIRULENCE OF THE LMPRL-1 DELETED L M AJOR
STRAIN IN A MURINE MODEL OF CUTANEOUS
LEISHM
ANIASIS..........................................................................................................................................................................................
- 1 0 3 -
4.4.1 ROLE OF LMPRL-1 IN A MURINE MODEL OF HOST SUSCEPTIBILITY TO
CUTANEOUS LEISHMANIASIS
......................
-1 0 4 -
4 .4.2 ROLE OF LMPRL-1 IN A MURINE MODEL OF HOST RESISTANCE TO CUTANEOUS
LEISHMANIASIS
...........................
- 1 0 6 -
4 .5 FUNCTIONAL STUDY OF LMPRL IN THE CONTEXT OF MACROPHAGE CELL
SIGNALING
............................................................
-1 0 8 -
4.5.1 ANALYSIS OF A PUTATIVE INTERACTION BETWEEN LMPRL AND PRL PROTEINS
IN M ACROPHAGES
......................
-1 0 8 -
4.5.2 ANALYSIS OF THE INFLUENCE OF LMPRL ON ACTIN CYTOSKELETON DYNAMICS
IN MACROPHAGE CELL LINES
..........
-1 1 9 -
4 .5 .3 INFLUENCE OF LMPRL ON M APK SIGNALING IN M
ACROPHAGES.............................................................................-1
2 5 -
5 D IS C U S S IO N
..........................................................................................................................................................................................-
1 2 9 -
5.1 BIOCHEMICAL AND STRUCTURAL ANALOGY BETWEEN LMPRL AND HUMAN P R L
..............................................................-1 2 9 -
5.2 EXPRESSION OF LMPRL DURING THE LEISHMANIA LIFE
CYCLE............................................................................................-1
3 1 -
5.3 SECRETION AND SUBCELLULAR LOCALIZATION OF LM
PRL........................................................................................................
- 1 3 4 -
5.4 PRO-SURVIVAL EFFECT OF LMPRL ON L
M AJOR DURING
INFECTION.......................................................................................-
1 3 6 -
5.5 ALTERATION OF MACROPHAGE FUNCTION DUE TO POSSIBLE INTERACTION OF
LMPRL WITH HOST P R L ..............................- 1 3 9 -
5.6 ROLE OF LMPRL IN SUBVERSION OF HOST CELL SIGNALING PATHWAYS BY
LEISHMANIA PARASITES
...............................
-1 4 1 -
5.7 MODEL OF THE ROLE OF LMPRL PHOSPHATASES DURING THE LIFE CYCLE OF
LEISHMANIA PARASITES
............................
-1 4 3 -
6 C O N C LUDING RE M A RK S
.......................................................................................................................................................................-
1 4 5 -
7 R E FE REN C E S
.........................................................................................................................................................................................
- 147 -
8 A B B RE V IA TIO N S
...................................................................................................................................................................................
-1 5 7 -
9 A P P E N D IX
.............................................................................................................................................................................................
- 1 5 9 -
10 A C K N O W LE D G E M E N
T...........................................................................
- 1 6 1 -
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| language | English |
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| spelling | Leitherer, Sabine Verfasser aut Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major Erlangen ; Nürnberg 2017 161 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2017 Immunreaktion (DE-588)4133841-8 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Proteintyrosinphosphatase (DE-588)4311372-2 gnd rswk-swf Leishmania major (DE-588)4404287-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Leishmania major (DE-588)4404287-5 s Genexpression (DE-588)4020136-3 s Proteintyrosinphosphatase (DE-588)4311372-2 s Immunreaktion (DE-588)4133841-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029947180&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Leitherer, Sabine Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major Immunreaktion (DE-588)4133841-8 gnd Genexpression (DE-588)4020136-3 gnd Proteintyrosinphosphatase (DE-588)4311372-2 gnd Leishmania major (DE-588)4404287-5 gnd |
| subject_GND | (DE-588)4133841-8 (DE-588)4020136-3 (DE-588)4311372-2 (DE-588)4404287-5 (DE-588)4113937-9 |
| title | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |
| title_alt | Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |
| title_auth | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |
| title_exact_search | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |
| title_full | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg |
| title_fullStr | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg |
| title_full_unstemmed | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major vorgelegt von Sabine Leitherer aus Bamberg |
| title_short | Biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite Leishmania major |
| title_sort | biochemical and functional characterization of two tyrosine phosphatases expressed by the intracellular parasite leishmania major biochemische und funktionelle charakterisierung zweier tyrosin phosphatasen des intrazellularen parasiten leishmania major |
| title_sub | = Biochemische und funktionelle Charakterisierung zweier Tyrosin-Phosphatasen des intrazellulären Parasiten Leishmania major |
| topic | Immunreaktion (DE-588)4133841-8 gnd Genexpression (DE-588)4020136-3 gnd Proteintyrosinphosphatase (DE-588)4311372-2 gnd Leishmania major (DE-588)4404287-5 gnd |
| topic_facet | Immunreaktion Genexpression Proteintyrosinphosphatase Leishmania major Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029947180&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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