A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Ulm
2017
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IX, 84 Blätter Illustrationen, Diagramme |
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| 245 | 1 | 0 | |a A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 |c Simon Markus Langer |
| 264 | 1 | |a Ulm |c 2017 | |
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Record in the Search Index
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| adam_text | TABLE OF CONTENTS
TABLE OF
CONTENTS..................................................................................................................................IV
LIST OF
ABBREVIATIONS.............................................................................................................................
VI
1.
INTRODUCTION...........................................................................................................................................1
1.1 HIV-1 EPIDEMIOLOGY AND CLASSIFICATION
..........................................................................................
1
1.2 GENOMIC ORGANIZATION AND PROTEIN SYNTHESIS OF
HIV-1.................................................................2
1.3 EXPRESSION OF UNUSUAL FUSION PROTEINS OF
HIV-1...........................................................................5
1.4 THE ACCESSORY PROTEIN
VPU...........................................................................................................
10
1.5 THE REGULATORY PROTEIN
REV...........................................................................................................
12
1.6 SCOPE OF THE
STUDY........................................................................................................................
13
2. MATERIAL AND
METHODS.........................................................................................................................
15
2.1
MATERIALS.......................................................................................................................................
15
2.1.1 EUKARYOTIC
CELLS.....................................................................................................................
15
2.1.2 PROKARYOTIC
CELLS....................................................................................................................
16
2.1.3 NUCLEIC
ACIDS.........................................................................................................................
16
2.1.4
ENZYMES................................................................................................................................
19
2.1.5
MEDIA.....................................................................................................................................
20
2.1.6 REAGENTS AND
CONSUMABLES....................................................................................................20
2.1.7 SOLUTIONS AND
BUFFERS.............................................................................................................24
2.1.8
ANTIBODIES.............................................................................................................................25
2.1.9 K
ITS........................................................................................................................................
27
2.1.10 TECHNICAL
EQUIPMENT...........................................................................................................27
2.1.11 SOFTWARE AND
DATABASES........................................................................................................29
2.2
METHODS........................................................................................................................................31
2.2.1
CLONING..................................................................................................................................31
2.2.2 EUKARYOTIC CELL CULTURE AND
TRANSFECTION................................................................................35
2.2.3 PROKARYOTIC CELL
CULTURE..........................................................................................................37
2.2.4 VIROLOGICAL
METHODS..............................................................................................................38
2.2.5 MOLECULAR BIOLOGY
TECHNIQUES...............................................................................................
41
2.2.6 FLOW
CYTOMETRY......................................................................................................................
43
2.2.7 IN SILICO ANALYSES OF RNA
STRUCTURE.......................................................................................44
2.2.8 STATISTICAL
ANALYSIS.................................................................................................................44
3.
RESULTS................................................................................................................................................
45
3.1 MANY HIV-1 STRAINS HARBOR A REVL-VPU FUSION
GENE.....................................................................45
3.2 GENERATION OF ISOGENIC VIRUS PAIRS, DIFFERING SPECIFICALLY IN THEIR
CAPACITY TO EXPRESS REVL-VPU
46
3.3 FUSION GENE CONTAINING HIV-1 PROVIRUSES EXPRESS A REVL-VPU FUSION
PROTEIN
..........................
47
3.4 CO-EXPRESSION OF REVL-VPU AND VPU DECREASES CD4, TETHERIN, CDLD AND
NTB-A
DOWNMODULATION.................................................................................................................................
50
3.5 PROVIRUSES EXPRESSING A REVL-VPU FUSION PROTEIN DO NOT EXHIBIT A
DEFECT IN VPU FUNCTION
.......
52
3.6 THE REVL-VPU POLYMORPHISM DOES NOT AFFECT REV-DEPENDENT GENE
EXPRESSION
...........................
54
3.7 THE REVL-VPU POLYMORPHISM DOES NOT ENHANCE HIV-1 REPLICATION
FITNESS...................................56
3.8 MUTATIONS IN THE REVL-VPU INTERGENIC REGION AFFECT ENV
EXPRESSION.............................................58
3.9 NO EVIDENCE FOR ACQUISITION OR LOSS OF THE REVL-VPU FUSION GENE IN
INFECTED INDIVIDUALS OVER TIME
61
4.
DISCUSSION..........................................................................................................................................63
5. SUMMARY AND
CONCLUSION..................................................................................................................
71
6.
REFERENCES..........................................................................................................................................
73
7. APPENDIX................................................ 80
8. LIST OF
PUBLICATIONS.............................................................................................................................81
9.
ACKNOWLEDGEMENTS............................................................................................................................82
10. STATUTORY
DECLARATION.........................................................................................................................83
11.
FIN.....................................................................................................................................................84
|
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| spelling | Langer, Simon Markus Verfasser (DE-588)1136915370 aut A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 Simon Markus Langer Ulm 2017 IX, 84 Blätter Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Ulm 2017 HIV-Infektion (DE-588)4203852-2 gnd rswk-swf HIV (DE-588)4200792-6 gnd rswk-swf HIV-1 HIV infections / Virology Viral regulatory and accessory proteins Genes, vpu (DE-588)4113937-9 Hochschulschrift gnd-content HIV (DE-588)4200792-6 s HIV-Infektion (DE-588)4203852-2 s DE-604 Universität Ulm Sonstige (DE-588)30607-1 oth DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029910614&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Langer, Simon Markus A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 HIV-Infektion (DE-588)4203852-2 gnd HIV (DE-588)4200792-6 gnd |
| subject_GND | (DE-588)4203852-2 (DE-588)4200792-6 (DE-588)4113937-9 |
| title | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 |
| title_auth | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 |
| title_exact_search | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 |
| title_full | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 Simon Markus Langer |
| title_fullStr | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 Simon Markus Langer |
| title_full_unstemmed | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 Simon Markus Langer |
| title_short | A naturally occurring rev1-vpu fusion gene does not confer a fitness advantage to HIV-1 |
| title_sort | a naturally occurring rev1 vpu fusion gene does not confer a fitness advantage to hiv 1 |
| topic | HIV-Infektion (DE-588)4203852-2 gnd HIV (DE-588)4200792-6 gnd |
| topic_facet | HIV-Infektion HIV Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029910614&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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