The HPLC-MS handbook for practitioners:
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Weinheim
Wiley-VCH Verlag GmbH & Co. KGaA
[2017]
|
| Edition: | 1. Auflage |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVI, 233 Seiten Illustrationen, Diagramme, Portraits |
| ISBN: | 9783527343072 |
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MARC
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| 003 | DE-604 | ||
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| 245 | 1 | 0 | |a The HPLC-MS handbook for practitioners |c edited by Stavros Kromidas |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Weinheim |b Wiley-VCH Verlag GmbH & Co. KGaA |c [2017] | |
| 264 | 4 | |c © 2017 | |
| 300 | |a XVI, 233 Seiten |b Illustrationen, Diagramme, Portraits | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 0 | 7 | |a HPLC-MS |0 (DE-588)4414892-6 |2 gnd |9 rswk-swf |
| 653 | |a Chemie | ||
| 653 | |a Chemistry | ||
| 653 | |a Environmental Chemistry | ||
| 653 | |a Flüssigkeitschromatographie | ||
| 653 | |a HPLC-MS | ||
| 653 | |a LC/MS | ||
| 653 | |a Mass Spectrometry | ||
| 653 | |a Massenspektrometrie | ||
| 653 | |a Pharmaceutical & Medicinal Chemistry | ||
| 653 | |a Pharmazeutische u. Medizinische Chemie | ||
| 653 | |a Umweltchemie | ||
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a HPLC-MS |0 (DE-588)4414892-6 |D s |
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| 700 | 1 | |a Kromidas, Stavros |d 1954- |0 (DE-588)122124685 |4 edt | |
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| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe, Mobi |z 978-3-527-80918-9 |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe, oBook |z 978-3-527-80920-2 |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-029830807 | ||
Record in the Search Index
| _version_ | 1804177729936949248 |
|---|---|
| adam_text | CONTENTS
PREFACE XI
THE STRUCTU RE OF
HPLC-MS FOR PRACTITIONERS XIII
LIST OF CONTRIBUTORS X V
PART I OVERVIEW, PITFALLS, HARDWARE-REQUIREMENTS 1
1 STATE OF THE ART IN THE LC/MS 3
O. SCHMITZ
1.1 INTRODUCTION 3
1.2 IONIZATION METHODS AT ATMOSPHERIC PRESSURE 5
1.2.1 OVERVIEW OF API METHODS 6
1.2.2 ESI 6
1.2.3 APCI 8
1.2.4 APPI 9
1.2.5 APLI 10
1.2.6 DETERMINATION OF ION SUPPRESSION 11
1.2.7 BEST IONIZATION FOR EACH QUESTION 11
1.3 MASS ANALYZER 12
1.4 FUTURE DEVELOPMENTS 13
1.5 WHAT SHOULD YOU LOOK FOR WHEN BUYING A MASS SPECTROMETER? 14
REFERENCES 15
2 TECHNICAL ASPECTS AND PITFALLS OF LC/MS HYPHENATION 19
M,M. MARTIN
2.1 INSTRUMENTAL REQUIREMENTS FOR LC/MS ANALYSIS - CONFIGURING THE
RIGHT SYSTEM FOR YOUR ANALYTICAL CHALLENGE 20
2.1.1 (U)HPLC AND MASS SPECTROMETRY - NOT JUST A MERE FRONT-END 20
2.1.2 UHPLC SYSTEM OPTIMIZATION - GRADIENT DELAY AND EXTRA-COLUMN
VOLUMES 21
2.1.3 DOES YOUR MASS SPECTROMETER FIT YOUR PURPOSE? 33
2.1.4 DATA RATES AND CYCLE TIMES OF MODERN MASS SPECTROMETERS 38
2.1.5 COMPLEMENTARY INFORMATION BY ADDITIONAL DETECTORS
OR MASS SPECTROMETRY WON*T SAVE THE WORLD 39
2.2 LC/MS METHOD DEVELOPMENT AND HPLC METHOD ADAPTATION -
HOW TO MAKE MY LC FIT FOR MS? 43
2.2.1 METHOD DEVELOPMENT LC/MS - LC FITS THE MS PURPOSES 44
2.2.2 CONVERTING CLASSICAL HPLC METHODS INTO LC/MS 53
2.3 PITFALLS AND ERROR SOURCES - SOMETIMES THINGS DO GO WRONG 54
2.3.1 NOTSIGNAL AT ALL 54
2.3.2 INAPPROPRIATE ION SOURCE SETTINGS AND THEIR IMPACT
ON THE CHROMATOGRAM 56
2.3.3 ION SUPPRESSION 58
2.3.4 UNKNOWN MASS SIGNALS IN THE MASS SPECTRUM 59
2.3.5 INSTRUMENTAL REASONS FOR THE MISINTERPRETATION OF MASS SPECTRA 65
2.4 CONCLUSION 68
2.5 ABBREVIATIONS 69
REFERENCES 70
3 ASPECTS OF THE DEVELOPMENT OF METHODS IN LC/MS COUPLING 73
T TEUTENBERG, T HETZEL, C. PORTNER, S. WIESE, C. VOM EYSER, AND
/. TUERK
3.1 INTRODUCTION 73
3.2 FROM TARGET TO SCREENING ANALYSIS 74
3.2.1 TARGET ANALYSIS 74
3.2.2 SUSPECTED-TARGET SCREENING 74
3.2.3 NON-TARGET SCREENING 74
3.2.4 COMPARABLE OVERVIEW OF THE DIFFERENT ACQUISITION MODES 75
3.3 THE OPTIMIZATION OF PARAMETERS IN CHROMATOGRAPHY AND MASS
SPECTROMETRY 75
3.3.1 REQUIREMENTS AND RECOMMENDATIONS FOR HPLC/MS ANALYSIS TAKING
DIN 38407-47 AS AN EXAMPLE 75
3.3.2 THE DEFINITION OF CRITICAL PEAK PAIRS IN THE CONTEXT OF HPLC/MS
COUPLING 77
3.3.3 THE SEPARATION OF POLAR COMPONENTS FROM THE COLUMN VOID TIME 79
3.3.4 DETERMINING THE HPLC METHOD PARAMETERS USING THE EXAMPLE
OF THE SEPARATION OF SELECTED PHARMACEUTICALS 80
3.3.5 CARRYING OUT SCREENING EXPERIMENTS 84
3.3.6 EVALUATION OF THE DATA AND DISCUSSION OF THE INFLUENCING
PARAMETERS 86
3.3.7 USING SIMULATION SOFTWARE FOR FINE OPTIMIZATION 98
3.3.8 CHOOSING THE STATIONARY PHASE SUPPORT 99
3.3.9 THE INFLUENCE OF THE INNER COLUMN DIAMETER AND THE MOBILE PHASE
FLOW RATE 103
3.3.10 THE INFLUENCE OF THE INJECTION VOLUME 104
3.3.11 ESTABLISHING THE MASS SPECTROMETRIC PARAMETERS 115
3.3.12 OPTIMIZATION OF THE MASS SPECTROMETRIC PARAMETERS 117
3.3.13 QUANTIFICATION USING LC/MS 122
3.3.14 SCREENING USING LC/MS 128
3.3.15 MINIATURIZATION - LC/MS QUO VADIS? 132
REFERENCES 135
PART II TIPS, EXAMPLES, TRENDS 139
4 LC/MS FOR EVERYBODY/FOR EVERYTHING? - LC/MS TIPS 141
E MANDEL
4.1 INTRODUCTION 141
4.2 TIP NUMBER 1 142
4.2.1 CHOOSING THE RIGHT LC/MS INTERFACE 142
4.3 TIP NUMBER 2 748
4.3.1 WHICH MOBILE PHASES ARE COMPATIBLE WITH LC/MS? 148
4.4 TIP NUMBER 3 149
4.4.1 PHOSPHATE BUFFER - THE EXCEPTION 149
4.5 TIP NUMBER 4 150
4.5.1 PAIRED IONS 150
4.5.2 WHICH ANTIDOTE* IS AVAILABLE? 151
4.5.3 SUMMARY 152
4.6 TIP NUMBER 5 152
4.6.1 USING ADDITIVES TO ENHANCE ELECTROSPRAY IONIZATION 152
4.6.2 ADDITIVES FOR APCI 153
4.6.3 SUMMARY 154
4.7 TIP NUMBER 6 154
4.7.1 HOW CAN I ENHANCE SENSITIVITY OF DETECTION? 154
4.8 TIP NUMBER 7 155
4.8.1 NO LINEAR RESPONSE AND POOR DYNAMIC RANGE? 155
4.8.2 THE REASONS
156
4.8.3 POSSIBLE SOLUTIONS 156
4.8.4 SUMMARY 157
4.9 TIP NUMBER 8 157
4.9.1 HOW MUCH MS DO I NEED? 157
4.9.2 SOLUTIONS 158
4.9.3 SUMMARY 158
4.10 NEED MORE HELP? 166
REFERENCES 167
PART III USER REPORTS 169
5 LC COUPLED TO MS - A USER REPORT 171
A. MULLER AND A. HOFMANN
REFERENCES 176
6 PROBLEM SOLVING WITH HPLC/MS-A PRACTICAL VIEW FROM PRACTITIONERS 177
E. FLEISCHER
6.1 INTRODUCTION AND SCOPE 177
6.2 CASE EXAMPLE 1 181
6.2.1 INVESTIGATION OF METHOHEXITAL IMPURITIES AND DECOMPOSITION
PRODUCTS 181
6.2.2 SAMPLE PREPARATION 181
6.3 CASE EXAMPLE 2 183
6.3.1 SEPARATION OF OLIGOMERS FROM CAPROLACTAM, MULTICOMPONENT
SEPARATION OF IMPURITIES ON A GRAM SCALE 183
6.4 CASE EXAMPLE 3 184
6.4.1 PREPARATION AND ISOLATION OF BIS-NALBUPHINE FROM NALBUPHINE 184
6.5 CASE EXAMPLE 4 186
6.5.1 ISOLATION AND ELUCIDATION OF DOPAMINE IMPURITIES 186
7 LC/MS FROM THE PERSPECTIVE OF A MAINTENANCE ENGINEER 189
O. ATO/ZER
7.1 INTRODUCTION AND HISTORICAL SUMMARY 189
7.2 SPRAY TECHNIQUES 190
7.3 PASSAGE THROUGH THE ION PATH 191
7.4 THE ANALYZER 191
7.5 MAINTENANCE 193
REFERENCES 198
PART IV VENDOR S REPORTS 201
8 LC/MS - THE PAST, PRESENT, AND FUTURE 203
T.L. SHEEHAN AND E MANDEL
9 VENDOR S REPORT - SCIEX 207
D. SCHLEUDER
10 MANUFACTURER REPORT - THERMO FISHER SCIENTIFIC 213
M M . MARTIN
10.1 LIQUID CHROMATOGRAPHY FOR LC/MS 214
10.2 MASS SPECTROMETRY FOR LC/MS 215
10.3 INTEGRATED LC/MS SOLUTIONS 217
10.4 SOFTWARE 217
REFERENCES 219
ABOUT THE AUTHORS 221
INDEX 227
|
| any_adam_object | 1 |
| author2 | Kromidas, Stavros 1954- |
| author2_role | edt |
| author2_variant | s k sk |
| author_GND | (DE-588)122124685 |
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| classification_rvk | VG 7500 VG 9800 WC 3480 |
| ctrlnum | (OCoLC)1002231000 (DE-599)DNB1123661022 |
| dewey-full | 540 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 540 - Chemistry and allied sciences |
| dewey-raw | 540 |
| dewey-search | 540 |
| dewey-sort | 3540 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie |
| edition | 1. Auflage |
| format | Book |
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| indexdate | 2024-07-10T07:52:43Z |
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| physical | XVI, 233 Seiten Illustrationen, Diagramme, Portraits |
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| spelling | The HPLC-MS handbook for practitioners edited by Stavros Kromidas 1. Auflage Weinheim Wiley-VCH Verlag GmbH & Co. KGaA [2017] © 2017 XVI, 233 Seiten Illustrationen, Diagramme, Portraits txt rdacontent n rdamedia nc rdacarrier HPLC-MS (DE-588)4414892-6 gnd rswk-swf Chemie Chemistry Environmental Chemistry Flüssigkeitschromatographie HPLC-MS LC/MS Mass Spectrometry Massenspektrometrie Pharmaceutical & Medicinal Chemistry Pharmazeutische u. Medizinische Chemie Umweltchemie (DE-588)4143413-4 Aufsatzsammlung gnd-content HPLC-MS (DE-588)4414892-6 s DE-604 Kromidas, Stavros 1954- (DE-588)122124685 edt Wiley-VCH (DE-588)16179388-5 pbl Erscheint auch als Online-Ausgabe, ePDF 978-3-527-80919-6 Erscheint auch als Online-Ausgabe, ePub 978-3-527-80917-2 Erscheint auch als Online-Ausgabe, Mobi 978-3-527-80918-9 Erscheint auch als Online-Ausgabe, oBook 978-3-527-80920-2 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029830807&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The HPLC-MS handbook for practitioners HPLC-MS (DE-588)4414892-6 gnd |
| subject_GND | (DE-588)4414892-6 (DE-588)4143413-4 |
| title | The HPLC-MS handbook for practitioners |
| title_auth | The HPLC-MS handbook for practitioners |
| title_exact_search | The HPLC-MS handbook for practitioners |
| title_full | The HPLC-MS handbook for practitioners edited by Stavros Kromidas |
| title_fullStr | The HPLC-MS handbook for practitioners edited by Stavros Kromidas |
| title_full_unstemmed | The HPLC-MS handbook for practitioners edited by Stavros Kromidas |
| title_short | The HPLC-MS handbook for practitioners |
| title_sort | the hplc ms handbook for practitioners |
| topic | HPLC-MS (DE-588)4414892-6 gnd |
| topic_facet | HPLC-MS Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029830807&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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