Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Aachen
Apprimus Verlag
2017
|
| Edition: | 1. Auflage |
| Series: | Ergebnisse aus den Naturwissenschaften
11 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | II, 97, XVIII Seiten Illustrationen, Diagramme 21 cm |
| ISBN: | 9783863595142 3863595149 |
Staff View
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV044420416 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 170721s2017 gw a||| m||| 00||| eng d | ||
| 015 | |a 17,N25 |2 dnb | ||
| 016 | 7 | |a 1135291349 |2 DE-101 | |
| 020 | |a 9783863595142 |c Broschur : EUR 39.00 (DE), EUR 40.10 (AT) |9 978-3-86359-514-2 | ||
| 020 | |a 3863595149 |9 3-86359-514-9 | ||
| 024 | 3 | |a 9783863595142 | |
| 035 | |a (OCoLC)1002252487 | ||
| 035 | |a (DE-599)DNB1135291349 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-NW | ||
| 049 | |a DE-29T |a DE-83 | ||
| 082 | 0 | |a 500 |2 23 | |
| 084 | |a 500 |2 sdnb | ||
| 100 | 1 | |a Beiß, Véronique |e Verfasser |0 (DE-588)1136736816 |4 aut | |
| 245 | 1 | 0 | |a Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum |c vorgelegt von Véronique Beiß |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2017 | |
| 300 | |a II, 97, XVIII Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Ergebnisse aus den Naturwissenschaften |v 11 | |
| 502 | |b Dissertation |c Rheinisch-Westfälische Technische Hochschule Aachen |d 2017 | ||
| 650 | 0 | 7 | |a Impfstoff |0 (DE-588)4026655-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Malaria |0 (DE-588)4037197-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Plasmodium falciparum |0 (DE-588)4250521-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Antigen |0 (DE-588)4002272-9 |2 gnd |9 rswk-swf |
| 653 | |a Fachhochschul-/Hochschulausbildung | ||
| 653 | |a Erwachsenenbildung | ||
| 653 | |a Biologie | ||
| 653 | |a Naturwissenschaften | ||
| 653 | |a Plasmodium falciparum | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Malaria |0 (DE-588)4037197-9 |D s |
| 689 | 0 | 1 | |a Impfstoff |0 (DE-588)4026655-2 |D s |
| 689 | 0 | 2 | |a Plasmodium falciparum |0 (DE-588)4250521-5 |D s |
| 689 | 0 | 3 | |a Antigen |0 (DE-588)4002272-9 |D s |
| 689 | 0 | |5 DE-604 | |
| 710 | 2 | |a Apprimus Verlag |0 (DE-588)1068101474 |4 pbl | |
| 830 | 0 | |a Ergebnisse aus den Naturwissenschaften |v 11 |w (DE-604)BV041770386 |9 11 | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u http://d-nb.info/1135291349/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029822031&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-029822031 | ||
Record in the Search Index
| _version_ | 1804177715342868480 |
|---|---|
| adam_text | TABLE OF CONTENTS
1
INTRODUCTION.........................................................................................................................
1
1.1 M
ALARIA...........................................................................................................................................................1
1.2 LIFE CYCLE OF PLASMODIUM FALCIPARUM
....................
1.3 VACCINES AGAINST MALARIA
.........................................
1.3.1 PRE-ERYTHROCYTIC VACCIN ES
..............................
1.3.2 BLOOD STAGE VACCINES.......................................
1.3.3 TRANSMISSION-BLOCKING VACCINES
...................
1.3.3.1 THE TB V CANDIDATE PFS48/45
....................
1.3.3.2 THE TB V CANDIDATE PFS230
.......................
1.3.3.3 THE TBV CANDIDATE P /G A P 50
...................
1.3.3.4 SEXUAL STAGE FUSION VACCINE CANDIDATES
1.3.3.5 TB V CANDIDATES IN (PRE-) CLINICAL
TRIALS....................................................................................
10
1.4 PLANTS AS PRODUCTION
PLATFORMS................................................................................................................10
1.5 OBJECTIVES OF THE
RESEARCH........................................................................................................................11
2 MATERIALS AND
METHODS......................................................................................................15
2.1 CHEMICALS AND
CONSUMABLES..................................................................................................................
15
2.2 ENZYMES, ANTIBODIES AND REACTION KITS
................................................................................................
15
2.3 MEDIA, SOLUTION AND
BUFFERS....................................................................................................................
16
2.4
VECTORS..........................................................................................................................................................
IS
2.5 GENE
VARIANTS.............................................................................................................................................20
2.6 SYNTHETIC
OLIGONUCLEOTIDES.......................................................................................................................20
2.7 ORGANISM
S..................................................................................................................................................
22
2.8 MOLECULAR BIOLOGICAL M
ETHODS................................................................................................................
22
2.8.1 CULTIVATION OF BACTERIA AND PREPARATION OF GLYCEROLSTOCKS
......................................................
22
2.8.2 PREPARATION OF CHEMICAL OR ELECTROCOMPATENT
CELLS..................................................................22
2.8.3 HEAT TRANSFORMATION OF E. COLI AND ELECTROPORATION OF A.
TUMEFACIENS................................23
2.8.4 PLASMID ISOLATION FROM E. COLI AND RESTRICTION OF D M A
............................................................. 23
2.8.5 RNA ISOLATION, CDNA AMPLIFICATION AND LINKER
ATTACHMENT.....................................................23
2.8.6 AGAROSE GEL ELECTROPHORESIS AND ISOLATION OF
DNA-FRAGMENTS..............................................24
2.8.7
LIGATION...............................................................................................................................................
24
2.8.8 POLYMERASE CHAIN REACTION (PCR), 5*RACE PCR AND SANGER SEQUENCING
.....................
24
2.9 PROTEIN CHEMICAL AND IMMUNOLOGICAL M
ETHODS..................................................................................
26
2.9.1 PROTEIN EXTRACTION AND
PURIFICATION...............................................................................................
26
2.9.2 IMMUNOAFFINITY CHROMATOGRAPHY (IA C
)........................................................................................
26
2.9.3 SDS-PAG E AND IMMUNOBLOT
ANALYSIS........................................................................................27
2.9.4 ENZYME-LINKED IMMUNOSORBENT
ASSAY........................................................................................27
2.9.5 SURFACE PLASMON RESONANCE
MEASUREMENT...............................................................................28
2.9.6 BRADFORD AND BCA
ASSAY...............................................................................................................
28
2.9.7 PROTEIN QUANTIFICATION USING FLUORESCENCE MEASUREMENT
.......................................................
29
2.10
PLANTS............................................................................................................................................................29
2.10.1 CULTIVATION OF NICOTIANA
BENTHAMIANA.........................................................................................
29
2.10.2 TRANSIENT PROTEIN EXPRESSION IN N. BENTHAMIANA
.....................................................................
29
2.11 EXPERIMENTS WITH HUMAN PLASMA, ANIMALS AND PLASMODIUM FALCIPARUM
......................................29
2.11.1 PLASMA REACTIVITIES OF AFRICAN SERUM SAM
PLES..........................................................................29
2.11.2 IMMUNIZATION OF MICE AND RABBITS AND DETERMINATION OF
TITERS..............................................30
2.11.3 GENERATION OF HYBRIDOMA CELL LINES AND HYBRIDOMA
RESCUE...................................................30
2.11.4 ASSAYS WITH P.
FALCIPAMM..............................................................................................................
31
3
RESULTS................................................................................................................................
33
3.1
P/S48/45.......................................................................................................................................................
33
3.1.1 CLONING OF EXPRESSION VECTORS FOR
PFS48/45...............................................................................33
3.1.2 EXPRESSION OF PFS48/45 GLYCO-KNOCKOUT VARIANTS
.....................................................................
36
3.1.3 EXPRESSION OF GLYCOSYLATED PFS48/45
VARIANTS............................................................................36
3.2
PFS230...........................................................................................................................................................41
3.2.1 CLONING OF EXPRESSION VECTORS FOR P
FS230..................................................................................
41
3.2.2 TRANSIENT EXPRESSION IN
PLANTS......................................................................................................42
3.2.3 DETERMINATION OF ANTIGEN-SPECIFIC TITERS AGAINST P /S230_C 0
.................................................
43
3.2.4 IMMUNOFLUORESCENCE
ASSAY...........................................................................................................44
3.3 P /G A P
50......................................................................................................................................................
46
D (O CO N O) O L O L A IS)
3.3.1 CLONING OF EXPRESSION VECTORS FOR
P/GAPSO...............................................................................46
3.3.2 EXPRESSION IN PLANTS AND
PURIFICATION...........................................................................................47
3.3.3 DETERMINATION OF ANTIGEN-SPECIFIC TITERS IN
RABBITS....................................................................
49
3.3.4 IMMUNOFLUORESCENCE
ASSAYS.........................................................................................................49
3.3.5 ZYGOTE INHIBITION ASSAY (Z IA
).........................................................................................................
50
3.3.6 TRANSMISSION-BLOCKING ASSAY (T B A
)............................................................................................
51
3.4 SEXUAL STAGE FUSION
PROTEINS..................................................................................................................
53
3.4.1 CLONING OF SEXUAL STAGE FUSION CONSTRUCTS
.................................................................................
53
3.4.2 EXPRESSION IN PLANTS AND
PURIFICATION...........................................................................................55
3.4.3 DETERMINATION OF ANTIGEN-SPECIFIC
TITERS......................................................................................
57
3.4.4 PLASMA REACTIVITY FROM AFRICAN SERUM
SAMPLES.........................................................................
58
3.4.5 IMMUNOFLUORESCENCE
ASSAYS.........................................................................................................
59
3.4.6 T RANSMISSION-BLOCKING
ASSAY.........................................................................................................60
3.5 ANTIBODIES AGAINST SINGLE SEXUAL STAGE
PROTEINS................................................................................
62
3.5.1 THE PFS25-SPECIFIC MOUSE MONOCLONAL ANTIBODY 4 B 7
.............................................................
62
3.5.1.1 CLONING SEQUENCES ENCODING THE MOUSE MONOCLONAL ANTIBODY 4 B 7
...............................63
3.5.1.2 EXPRESSION IN PLANTS AND
PURIFICATION......................................................................................
64
3.5.1.3 IMMUNOAFFINITY CHROMATOGRAPHY WITH PLANT-DERIVED M
AB4B7............................................65
3.5.2 GENERATION OF MONOCLONAL ANTIBODIES AGAINST PFS230, PFS25 AND PIC
E LT O S
...................
66
3.5.3 HYBRIDOMA RESCUE OF ANTIBODIES AGAINST PFS230, PFS25 AND P /C E LT
O S
...........................
67
4 DISCUSSION AND
CONCLUSION..............................................................................................
69
4.1 EVALUATION OF P
/S48/45...............................................................................................................................69
4.2 EVALUATION OF P
/S230..................................................................................................................................71
4.3 EVALUATION OF
PK3A P
50.............................................................................................................................
72
4.4 EVALUATION OF SEXUAL STAGE FUSION
PROTEINS..........................................................................................74
4.5 EVALUATION OF ANTIBODIES AGAINST SINGLE SEXUAL STAGE
ANTIGENS..................................................... 76
4.6 GENERAL CONCLUSION AND
OUTLOOK............................................................................................................
76
5
SUMMARY.............................................................................................................................
83
6 DEUTSCHE
ZUSAMMENFASSUNG..........................................................................................85
7
REFERENCES..........................................................................................................................87
8
APPENDIX................................................................................................................................
I
8.1 REGISTER OF
FIGURES..........................................................................................................................................
I
8.2 REGISTER OF
TABLES..........................................................................................................................................
II
6.3
ABBREVIATIONS................................................................................................................................................ILL
6.4 SUPPLEMENTAL
FIGURES.................................................................................................................................
VI
CURRICULUM
VITAE......................................................................................................................XVII
|
| any_adam_object | 1 |
| author | Beiß, Véronique |
| author_GND | (DE-588)1136736816 |
| author_facet | Beiß, Véronique |
| author_role | aut |
| author_sort | Beiß, Véronique |
| author_variant | v b vb |
| building | Verbundindex |
| bvnumber | BV044420416 |
| ctrlnum | (OCoLC)1002252487 (DE-599)DNB1135291349 |
| dewey-full | 500 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 500 - Natural sciences and mathematics |
| dewey-raw | 500 |
| dewey-search | 500 |
| dewey-sort | 3500 |
| dewey-tens | 500 - Natural sciences and mathematics |
| discipline | Allgemeine Naturwissenschaft |
| edition | 1. Auflage |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02461nam a2200601 cb4500</leader><controlfield tag="001">BV044420416</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">170721s2017 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">17,N25</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1135291349</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783863595142</subfield><subfield code="c">Broschur : EUR 39.00 (DE), EUR 40.10 (AT)</subfield><subfield code="9">978-3-86359-514-2</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">3863595149</subfield><subfield code="9">3-86359-514-9</subfield></datafield><datafield tag="024" ind1="3" ind2=" "><subfield code="a">9783863595142</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1002252487</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1135291349</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-NW</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29T</subfield><subfield code="a">DE-83</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">500</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">500</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Beiß, Véronique</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1136736816</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum</subfield><subfield code="c">vorgelegt von Véronique Beiß</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. Auflage</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Aachen</subfield><subfield code="b">Apprimus Verlag</subfield><subfield code="c">2017</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">II, 97, XVIII Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">21 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Ergebnisse aus den Naturwissenschaften</subfield><subfield code="v">11</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Rheinisch-Westfälische Technische Hochschule Aachen</subfield><subfield code="d">2017</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Impfstoff</subfield><subfield code="0">(DE-588)4026655-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Malaria</subfield><subfield code="0">(DE-588)4037197-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Plasmodium falciparum</subfield><subfield code="0">(DE-588)4250521-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Antigen</subfield><subfield code="0">(DE-588)4002272-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Fachhochschul-/Hochschulausbildung</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Erwachsenenbildung</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Biologie</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Naturwissenschaften</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Plasmodium falciparum</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Malaria</subfield><subfield code="0">(DE-588)4037197-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Impfstoff</subfield><subfield code="0">(DE-588)4026655-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Plasmodium falciparum</subfield><subfield code="0">(DE-588)4250521-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Antigen</subfield><subfield code="0">(DE-588)4002272-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="710" ind1="2" ind2=" "><subfield code="a">Apprimus Verlag</subfield><subfield code="0">(DE-588)1068101474</subfield><subfield code="4">pbl</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Ergebnisse aus den Naturwissenschaften</subfield><subfield code="v">11</subfield><subfield code="w">(DE-604)BV041770386</subfield><subfield code="9">11</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://d-nb.info/1135291349/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029822031&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-029822031</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV044420416 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:52:29Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783863595142 3863595149 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029822031 |
| oclc_num | 1002252487 |
| open_access_boolean | |
| owner | DE-29T DE-83 |
| owner_facet | DE-29T DE-83 |
| physical | II, 97, XVIII Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2017 |
| publishDateSearch | 2017 |
| publishDateSort | 2017 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Ergebnisse aus den Naturwissenschaften |
| series2 | Ergebnisse aus den Naturwissenschaften |
| spelling | Beiß, Véronique Verfasser (DE-588)1136736816 aut Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum vorgelegt von Véronique Beiß 1. Auflage Aachen Apprimus Verlag 2017 II, 97, XVIII Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Ergebnisse aus den Naturwissenschaften 11 Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2017 Impfstoff (DE-588)4026655-2 gnd rswk-swf Malaria (DE-588)4037197-9 gnd rswk-swf Plasmodium falciparum (DE-588)4250521-5 gnd rswk-swf Antigen (DE-588)4002272-9 gnd rswk-swf Fachhochschul-/Hochschulausbildung Erwachsenenbildung Biologie Naturwissenschaften Plasmodium falciparum (DE-588)4113937-9 Hochschulschrift gnd-content Malaria (DE-588)4037197-9 s Impfstoff (DE-588)4026655-2 s Plasmodium falciparum (DE-588)4250521-5 s Antigen (DE-588)4002272-9 s DE-604 Apprimus Verlag (DE-588)1068101474 pbl Ergebnisse aus den Naturwissenschaften 11 (DE-604)BV041770386 11 B:DE-101 application/pdf http://d-nb.info/1135291349/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029822031&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Beiß, Véronique Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum Ergebnisse aus den Naturwissenschaften Impfstoff (DE-588)4026655-2 gnd Malaria (DE-588)4037197-9 gnd Plasmodium falciparum (DE-588)4250521-5 gnd Antigen (DE-588)4002272-9 gnd |
| subject_GND | (DE-588)4026655-2 (DE-588)4037197-9 (DE-588)4250521-5 (DE-588)4002272-9 (DE-588)4113937-9 |
| title | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum |
| title_auth | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum |
| title_exact_search | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum |
| title_full | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum vorgelegt von Véronique Beiß |
| title_fullStr | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum vorgelegt von Véronique Beiß |
| title_full_unstemmed | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum vorgelegt von Véronique Beiß |
| title_short | Multi-target malaria vaccines against the sexual stage of Plasmodium falciparum |
| title_sort | multi target malaria vaccines against the sexual stage of plasmodium falciparum |
| topic | Impfstoff (DE-588)4026655-2 gnd Malaria (DE-588)4037197-9 gnd Plasmodium falciparum (DE-588)4250521-5 gnd Antigen (DE-588)4002272-9 gnd |
| topic_facet | Impfstoff Malaria Plasmodium falciparum Antigen Hochschulschrift |
| url | http://d-nb.info/1135291349/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029822031&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV041770386 |
| work_keys_str_mv | AT beißveronique multitargetmalariavaccinesagainstthesexualstageofplasmodiumfalciparum AT apprimusverlag multitargetmalariavaccinesagainstthesexualstageofplasmodiumfalciparum |
There is no print copy available.
Indexes