Fed-batch fermentation a practical guide to scalable recombinant protein production in escherichia coli - Moulton, Garner G. (Woodhead Publishing, 2014)
Previously issued in print: Witney: Biohealthcare, 2013
Includes bibliographical references (pages 151-164) and index
Primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R & D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision