Enzymology in vivo:
Gespeichert in:
| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Greenwich, Conn.
JAI Press
1995
|
| Schriftenreihe: | Advances in molecular and cell biology
v. 11 |
| Schlagworte: | |
| Online-Zugang: | FAW01 Volltext |
| Beschreibung: | Includes bibliographical references and index This volume describes some of the approaches that have been used to study enzymes in vivo. Metabolic control analysis provides a relatively simple framework with which to relate flux in a metabolic pathway to the kinetic properties of the component enzymes. More importantly it shows us how the importance of an enzyme in controlling flux in a pathway can be quantitated experimentally from measurements on the intact tissue. Fluorescence microscopy and NMR are two spectroscopic techniques which can be used to monitor, non-invasively, metabolite levels, metabolic fluxes and enzyme localization and mobility in intact biological systems. The potential of NMR for investigating the properties of enzymes in vivo has been greatly enhanced by using the technique in conjunction with molecular genetic method for changing the levels and properties of specific enzymes in the intact cell. Control of metabolism is regarded by some as a dead subject, with little new to learn. While it is true that the chemistry of the major metabolic pathways have been fully elucidated, our understanding of how they are controlled in the cell is still rather limited. Of particular interest is the emerging evidence for a high degree of spatial organization of the supposedly 'soluble' enzymes in the cytosol and the mitochondrial matrix. Much is still to be learnt on how this organization is effected and what influence it has on control of metabolic flux. If this volume excites some interest in this area of research and, furthermore, demonstrates that these problems are eminently addressable using the new techniques which are being developed, then it will have served a useful purpose |
| Beschreibung: | x, 271 pages |
| ISBN: | 1559388447 9781559388443 |
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| 490 | 0 | |a Advances in molecular and cell biology |v v. 11 | |
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| 500 | |a This volume describes some of the approaches that have been used to study enzymes in vivo. Metabolic control analysis provides a relatively simple framework with which to relate flux in a metabolic pathway to the kinetic properties of the component enzymes. More importantly it shows us how the importance of an enzyme in controlling flux in a pathway can be quantitated experimentally from measurements on the intact tissue. Fluorescence microscopy and NMR are two spectroscopic techniques which can be used to monitor, non-invasively, metabolite levels, metabolic fluxes and enzyme localization and mobility in intact biological systems. The potential of NMR for investigating the properties of enzymes in vivo has been greatly enhanced by using the technique in conjunction with molecular genetic method for changing the levels and properties of specific enzymes in the intact cell. Control of metabolism is regarded by some as a dead subject, with little new to learn. While it is true that the chemistry of the major metabolic pathways have been fully elucidated, our understanding of how they are controlled in the cell is still rather limited. Of particular interest is the emerging evidence for a high degree of spatial organization of the supposedly 'soluble' enzymes in the cytosol and the mitochondrial matrix. Much is still to be learnt on how this organization is effected and what influence it has on control of metabolic flux. If this volume excites some interest in this area of research and, furthermore, demonstrates that these problems are eminently addressable using the new techniques which are being developed, then it will have served a useful purpose | ||
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| spelling | Enzymology in vivo Series editor, E. Edward Bittar, Guest ed.: Kevin M. Brindle Greenwich, Conn. JAI Press 1995 x, 271 pages txt rdacontent c rdamedia cr rdacarrier Advances in molecular and cell biology v. 11 Includes bibliographical references and index This volume describes some of the approaches that have been used to study enzymes in vivo. Metabolic control analysis provides a relatively simple framework with which to relate flux in a metabolic pathway to the kinetic properties of the component enzymes. More importantly it shows us how the importance of an enzyme in controlling flux in a pathway can be quantitated experimentally from measurements on the intact tissue. Fluorescence microscopy and NMR are two spectroscopic techniques which can be used to monitor, non-invasively, metabolite levels, metabolic fluxes and enzyme localization and mobility in intact biological systems. The potential of NMR for investigating the properties of enzymes in vivo has been greatly enhanced by using the technique in conjunction with molecular genetic method for changing the levels and properties of specific enzymes in the intact cell. Control of metabolism is regarded by some as a dead subject, with little new to learn. While it is true that the chemistry of the major metabolic pathways have been fully elucidated, our understanding of how they are controlled in the cell is still rather limited. Of particular interest is the emerging evidence for a high degree of spatial organization of the supposedly 'soluble' enzymes in the cytosol and the mitochondrial matrix. Much is still to be learnt on how this organization is effected and what influence it has on control of metabolic flux. If this volume excites some interest in this area of research and, furthermore, demonstrates that these problems are eminently addressable using the new techniques which are being developed, then it will have served a useful purpose Enzymology fast Enzymology In vivo (DE-588)4250700-5 gnd rswk-swf Enzymologie (DE-588)4152482-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content 2\p (DE-588)1071861417 Konferenzschrift gnd-content Enzymologie (DE-588)4152482-2 s In vivo (DE-588)4250700-5 s 3\p DE-604 Bittar, E. E. Sonstige oth Brindle, Kevin M. Sonstige oth http://www.sciencedirect.com/science/bookseries/15692558/11 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Enzymology in vivo Enzymology fast Enzymology In vivo (DE-588)4250700-5 gnd Enzymologie (DE-588)4152482-2 gnd |
| subject_GND | (DE-588)4250700-5 (DE-588)4152482-2 (DE-588)4143413-4 (DE-588)1071861417 |
| title | Enzymology in vivo |
| title_auth | Enzymology in vivo |
| title_exact_search | Enzymology in vivo |
| title_full | Enzymology in vivo Series editor, E. Edward Bittar, Guest ed.: Kevin M. Brindle |
| title_fullStr | Enzymology in vivo Series editor, E. Edward Bittar, Guest ed.: Kevin M. Brindle |
| title_full_unstemmed | Enzymology in vivo Series editor, E. Edward Bittar, Guest ed.: Kevin M. Brindle |
| title_short | Enzymology in vivo |
| title_sort | enzymology in vivo |
| topic | Enzymology fast Enzymology In vivo (DE-588)4250700-5 gnd Enzymologie (DE-588)4152482-2 gnd |
| topic_facet | Enzymology In vivo Enzymologie Aufsatzsammlung Konferenzschrift |
| url | http://www.sciencedirect.com/science/bookseries/15692558/11 |
| work_keys_str_mv | AT bittaree enzymologyinvivo AT brindlekevinm enzymologyinvivo |