Eukaryotic transcriptional and post-transcriptional gene expression regulation:
Saved in:
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York
Humana Press
[2017]
|
| Series: | Methods in molecular biology
1507 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xi, 278 Seiten Illustrationen |
| ISBN: | 9781493965168 |
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Record in the Search Index
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| adam_text | Titel: Eukaryotic transcriptional and post-transcriptional gene expression regulation
Autor: Wajapeyee, Narendra
Jahr: 2017
Contents
Preface.......................................................... v
Contributors.......................................................... ix
1 Fluorescence Reporter-Based Genome-Wide RNA Interference
Screening to Identify Alternative Splicing Regulators.................... 1
Ashish Misra and Michael R. Green
2 Tandem Affinity Purification Approach Coupled to Mass Spectrometry
to Identify Post-translational Modifications of Histones Associated
with Chromatin-Binding Proteins.................................. 13
Sophie Beyer, Philippe Robin, and Slimane Ait-Si-Ali
3 Efficient Preparation of High-Complexity ChlP-Seq Profiles
from Early Xenopus Embryos..................................... 23
George E. Gentsch and James C. Smith
4 Systematic Discovery of Chromatin-Bound Protein Complexes
from ChlP-seq Datasets......................................... 43
Eugenia Giannopoulou and Olivier Elemento
5 Assay for Transposase-Accessible Chromatin with High-Throughput
Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos................ 59
Canan Doganli, Melissa Sandoval, Sean Thomas, and Daniel Hart
6 Establishment of Time- and Cell-Specific RNAi in Caenorhabditis elegans .... 67
Masayuki Hamakawa and Takaaki Hirotsu
7 Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein
and Guide RNA for Genome Editing............................... 81
Bharathi Suresh, Suresh Ramakrishna, and Hyongbum Kim
8 Epigenetic Analysis of Endocrine Cell Subtypes from Human
Pancreatic Islets............................................... 95
Jia Zhang and Klaus H. Kaestner
9 eIF3 Regulation of Protein Synthesis, Tumorigenesis,
and Therapeutic Response........................................ 113
Ji-Te Tin, Zizheng Dong, and Jian-Ting Zhang
10 High-Resolution Gene Expression Profiling of RNA Synthesis,
Processing, and Decay by Metabolic Labeling of Newly Transcribed
RNA Using 4-Thiouridine....................................... 129
Andrzej J. Rutkowski and Lars Dolken
11 Accurate Detection of Differential Expression and Splicing Using
Low-Level Features............................................. 141
Tomi Suomi and Laura L. Elo
vii
viii
Contents
12 Profiling Changes in Histone Post-translational Modifications
by Top-Down Mass Spectrometry.................................. 153
Mowei Zhou, Si Wu, David L. Stenoien, Zhaorui Zhang,
Lanelle Connolly, Michael Freitag, and Ljiljana Pasa-Tolic
13 Determining if an mRNA is a Substrate of Nonsense-Mediated
mRNA Decay in Saccharomyces cerevisiae............................. 169
Marcus J.O. Johansson
14 Optimizing In Vitro Pre-mRNA 3 Cleavage Efficiency: Reconstitution
from Anion-Exchange Separated HeLa Cleavage Factors
and from Adherent HeLa Cell Nuclear Extract........................ 179
Mihwa Na, Susana T. Valente, and Kevin Ryan
15 Unbiased Interrogation of 3D Genome Topology Using
Chromosome Conformation Capture Coupled to High-Throughput
Sequencing (4C-Seq)........................................... 199
Rutger W.W. Brouwer, Mir jam C.G.N, van den Hout,
Wilfred F.J. van IJcken, Eric Soler, and Ralph Stadhouders
16 Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect
Transcriptional Regulation in Human Embryonic Stem Cells............. 221
Krishna Mohan Parsi, Erica Hennessy, Nicola Kearns, and Rene Maehr
17 In Vitro Assay to Study Histone Ubiquitination During
Transcriptional Regulation....................................... 235
Jogender Tushir-Singh and Sanchita Bhatnagar
18 Determination of Alternate Splicing Events Using Transcriptome Arrays..... 245
Rebecca S. DeVaux, Marcy Kuentzel, Jason Herschkowitz,
and Sridar V. Chittur
19 Large-Scale RNA Interference Screening to Identify Transcriptional
Regulators of a Tumor Suppressor Gene............................. 261
Matteo Forloni, Thuy Ho, Lisha Sun, and Narendra Wajapeyee
20 Transcriptional Analysis-based Integrative Genomics Approach
to Identify Tumor Promoting Metabolic Genes........................ 269
Romi Gupta and Narendra Wajapeyee
Index............................................................... 277
|
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| indexdate | 2024-07-10T07:48:02Z |
| institution | BVB |
| isbn | 9781493965168 |
| language | English |
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| physical | xi, 278 Seiten Illustrationen |
| publishDate | 2017 |
| publishDateSearch | 2017 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Eukaryotic transcriptional and post-transcriptional gene expression regulation edited by Narendra Wajapeyee and Romi Gupta New York Humana Press [2017] xi, 278 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1507 Springer protocols Eukaryontische Zelle (DE-588)4153142-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf Posttranskriptionelle Regulation (DE-588)4356177-9 gnd rswk-swf eukaryotic cells eukaryotic gene expression regulation gene expression posttranscriptional gene expression regulation transcriptional (DE-588)4143413-4 Aufsatzsammlung gnd-content Eukaryontische Zelle (DE-588)4153142-5 s Genregulation (DE-588)4122166-7 s Posttranskriptionelle Regulation (DE-588)4356177-9 s Methode (DE-588)4038971-6 s DE-604 Wajapeyee, Narendra edt Gupta, Romi 1979- (DE-588)14338449X edt Erscheint auch als eBook 978-1-4939-6518-2 Methods in molecular biology 1507 (DE-604)BV035362695 1507 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029664822&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Eukaryotic transcriptional and post-transcriptional gene expression regulation Methods in molecular biology Eukaryontische Zelle (DE-588)4153142-5 gnd Methode (DE-588)4038971-6 gnd Genregulation (DE-588)4122166-7 gnd Posttranskriptionelle Regulation (DE-588)4356177-9 gnd |
| subject_GND | (DE-588)4153142-5 (DE-588)4038971-6 (DE-588)4122166-7 (DE-588)4356177-9 (DE-588)4143413-4 |
| title | Eukaryotic transcriptional and post-transcriptional gene expression regulation |
| title_auth | Eukaryotic transcriptional and post-transcriptional gene expression regulation |
| title_exact_search | Eukaryotic transcriptional and post-transcriptional gene expression regulation |
| title_full | Eukaryotic transcriptional and post-transcriptional gene expression regulation edited by Narendra Wajapeyee and Romi Gupta |
| title_fullStr | Eukaryotic transcriptional and post-transcriptional gene expression regulation edited by Narendra Wajapeyee and Romi Gupta |
| title_full_unstemmed | Eukaryotic transcriptional and post-transcriptional gene expression regulation edited by Narendra Wajapeyee and Romi Gupta |
| title_short | Eukaryotic transcriptional and post-transcriptional gene expression regulation |
| title_sort | eukaryotic transcriptional and post transcriptional gene expression regulation |
| topic | Eukaryontische Zelle (DE-588)4153142-5 gnd Methode (DE-588)4038971-6 gnd Genregulation (DE-588)4122166-7 gnd Posttranskriptionelle Regulation (DE-588)4356177-9 gnd |
| topic_facet | Eukaryontische Zelle Methode Genregulation Posttranskriptionelle Regulation Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029664822&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
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