Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Leipzig
Helmholtz-Zentrum für Umweltforschung (UFZ)
2016
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| Schriftenreihe: | PhD Dissertation
2016,6 |
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| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 172 Seiten Illustrationen, Diagramme |
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Datensatz im Suchindex
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| adam_text | Titel: Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial
Autor: Nguyen, Phuong Minh
Jahr: 2016
Microbial sulfur transformations in conslrnclctl wtlam/s
TABLE OF CONTENTS
SUMMARY..............................................................................................................................1
1. INTRODUCTION................................................................................................................9
1.1 Inorganic sulfur compounds in the environment.......................................................9
1.2 Sulfur transformations in constructed wetlands......................................................10
1.2.1 Dissimilatory sulfate reduction..............................................................................12
1.2.2 Àssimilatory sulfate reduction...............................................................................13
1.2.3 Mineral precipitation of sulfate..............................................................................14
1.2.4 Precipitation of metal sulfides...............................................................................14
1.2.5 Emission of hydrogen sulfide................................................................................15
1.2.6 Oxidation of sulfide...............................................................................................15
1.2.7 Sulfur disproportionation.......................................................................................16
1.2.8 Sulfur transformations in the interconnections with wetland plants and other
important redox processes..............................................................................................18
1.2.9 Sulfur transformations in constructed wetlands for sulfate-rich wastewater
treatment applications.....................................................................................................21
1.3 Sulfur oxidizing bacteria.............................................................................................22
1.3.1 Colorless sulfur oxidizing bacteria........................................................................22
1.3.2 Colored sulfur oxidizing bacteria..........................................................................26
1.3.3 Microbial sulfur oxidation pathways with gene systems involved........................29
1.3.4 Sulfur oxidizing bacteria in wastewater treatment......................... ......................32
1.4 Research gaps and objective of this study.................................................................34
2. MATERIALS AND METHODS......................................................................................36
2.1 Description of laboratory-scale constructed wetland models..................................36
2.2 Experimental set-up....................................................................................................37
2.3 Conceptual framework...............................................................................................39
2.4 Chemical analysis and calculations...........................................................................45
2.4.1 Sulfur compounds..................................................................................................45
2.4.2 TOC, acetate and benzoate....................................................................................45
2.4.3 Nitrogen species.........................................!...............................,...........................46
2.4.4 Redox potential, temperature and pH....................................................................46
2.4.5. Dissolved oxygen..................................................................................................46
3
Microbial sulfur iransjonnations in constructed wetlands
2.4.6 Calculations...........................................................................................................46
2.5 Molecular analysis.......................................................................................................48
2.5.1 DNA isolation and quantification..........................................................................48
2.5.2 PCR amplification of the 16S rRNA gene.............................................................49
2.5.3 Agarose gel electrophoresis...................................................................................50
2.5.4 454 Pyrosequencing...............................................................................................50
2.5.5 Quantitative PCR...................................................................................................57
3. RESULTS AND DISCUSSION........................................................................................58
3.1 Dynamics of sulfur compounds in wetland models..................................................58
3.2 Sulfur transformations in the interconnections with other important redox
processes.............................................................................................................................67
3.2.1 Nitrogen transformations.......................................................................................67
3.2.2 Behavior of organic carbon sources: acetate, benzoate, TOC...............................71
3.3 Other physicoçhemical parameters correlating with sulfur transformations.......74
3.3.1 pH...........................................................................................................................74
3.3.2 Redox potential......................................................................................................76
3.3.3 Dissolved oxygen...................................................................................................78
3.3.4 Effects of sulfide on growth of wetland plants......................................................79
3.4 Microbial community analysis in wetland models...................................................83
3.4.1 SOB and SRB abundance and the correlations with behavior of sulfur compounds
........................................................................................................................................83
3.4.2 Quantitative qPCR targeting 16S rRNA genes......................................................95
3.4.3 Microbial community compositions....................................................................100
4. CONCLUDING REMARKS...........................................................................................106
References.............................................................................................................................109
Appendix...............................................................................................................................123
Acknowledgement................................................................................................................168
4
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| spelling | Nguyen, Phuong Minh 1986- (DE-588)1124471332 aut Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater vorgelegt von Phuong Minh Nguyen Leipzig Helmholtz-Zentrum für Umweltforschung (UFZ) 2016 172 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier PhD Dissertation 2016,6 Dissertation Technische Universität Dresden 2016 Schwefeloxidierende Bakterien (DE-588)4416708-8 gnd rswk-swf Pflanzenkläranlage (DE-588)4136781-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Pflanzenkläranlage (DE-588)4136781-9 s Schwefeloxidierende Bakterien (DE-588)4416708-8 s DE-604 PhD Dissertation 2016,6 (DE-604)BV035421074 2016,6 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029601163&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nguyen, Phuong Minh 1986- Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater PhD Dissertation Schwefeloxidierende Bakterien (DE-588)4416708-8 gnd Pflanzenkläranlage (DE-588)4136781-9 gnd |
| subject_GND | (DE-588)4416708-8 (DE-588)4136781-9 (DE-588)4113937-9 |
| title | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater |
| title_auth | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater |
| title_exact_search | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater |
| title_full | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater vorgelegt von Phuong Minh Nguyen |
| title_fullStr | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater vorgelegt von Phuong Minh Nguyen |
| title_full_unstemmed | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater vorgelegt von Phuong Minh Nguyen |
| title_short | Microbial sulfur transformations in novel laboratory-scale constructed wetlands treating artificial wastewater |
| title_sort | microbial sulfur transformations in novel laboratory scale constructed wetlands treating artificial wastewater |
| topic | Schwefeloxidierende Bakterien (DE-588)4416708-8 gnd Pflanzenkläranlage (DE-588)4136781-9 gnd |
| topic_facet | Schwefeloxidierende Bakterien Pflanzenkläranlage Hochschulschrift |
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