Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Gießen
DVG Service GmbH
2016
|
| Edition: | 1. Auflage |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | iv, 145 Seiten Illustrationen, Diagramme 21 cm |
| ISBN: | 9783863453206 |
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Record in the Search Index
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|---|---|
| adam_text | University of Veterinary Medicine Hannover
Influence of a dietary-induced hypercholesterolemia
on the pathogenesis of Theiler s murine
encephalomyelitis with special emphasis on
transcriptional changes
INAUGURAL -DISSERTATION
in partial fulfillment of the requirements of the degree of
Doctor of Veterinary Medicine
-Doctor medicinae veterinariae -
(Dr med vet )
submitted by
Barbara Beate Rosa Raddatz
Vilshofen
Hannover 2016
Contents
1 Introduction 1
2 Literature review 3
2 1 Demyelinating diseases 3
211 Multiple sclerosis 3
212 Theiler s murine encephalomyelitis 3
213 Remyelination in demyelinating diseases 5
2 2 Cholesterol metabolism in the normal and diseased central nervous system 6
221 Physiological cholesterol metabolism in the central nervous system 6
222 Influence of circulatory cholesterol levels 9
223 Cholesterol in neuropathology 11
224 Cholesterol and the myelin sheath 12
225 The role of cholesterol in demyelinating diseases 13
2 3 Gene expression profiling using microarray technology 13
231 Biological background 14
232 Microarray technology 14
233 Minimum Information About a Microarray Experiment (MIAME) 15
3 Materials and Methods 17
3 1 Feeding Experiment 17
311 Animal housing 17
312 Experimental design 17
313 Anesthesia 19
314 Intracerebral infection 19
315 Clinical examination 19
316 Rotarod testing 20
317 Necropsy 20
318 Clinical chemistry 21
3 2 Tissue samples 21
321 Paraffin-embedded material 21
322 OCT-embedded material 21
323 Epoxy resin embedded material 21
3 3 Histochemical methods 22
331 Hematoxylin-Eosin-staining 22
332 Heidenhain’s azan trichrome stain 22
i
333 Fouchet’s stain 22
334 Luxol Fast Blue-/Cresyl Violet-stain 23
335 Oil red O stain 24
3 4 Light microscopic examination of tissue samples 24
341 Liver 24
342 Aorta and large pulmonary arteries 24
343 Spinal cord 25
3 5 Immunohistochemical methods 25
351 Protocol 25
352 Antibodies 26
3 6 High performance thin layer chromatography
(HPTLC) of liver and spinal cord 28
3 7 Statistical evalutation 28
3 8 Microarray experiment 28
4 Central nervous system de- and remyelination is independent from sys-
temic cholesterol level in Theiler s murine encephalomyelitis 31
5 An Application-oriented Introduction to Microarray-based Gene Ex-
pression Analysis for Veterinary Pathologists 33
5 1 Introduction 35
5 2 Experimental design and microarray hybridization 36
521 Experimental design 36
522 RNA isolation and quality control 38
523 RNA amplihcation, labeling, hybridization and microarray washing,
staining and scanning 39
5 3 Low level analysis 40
531 Quality control of microarray data 40
532 Low-level analysis 45
533 Annotation of microarray data 51
534 Principle components analysis 55
535 Top-down analysis of gene expression 56
536 Selection of differentially expressed genes 56
537 Cluster analysis 62
5 4 Functional annotation and pathway analysis 65
541 Gene Ontology 65
542 Enrichment analysis 66
543 Pathway analysis: 68
5 5 Bottom-up analysis of gene expression 70
5 6 Advanced methods of microarray analysis:
Biomarker discovery 72
ii
5 7 RNAseq based gene expression analysis 73
571 RNA sequencing 73
572 Raw data processing 76
573 Differences between microarray and RNAseq data 79
5 8 Conclusion 79
5 9 Acknowledgments 80
5 10 References 81
6 Discussion ^ 109
6 1 Cholesterol metabolism in Theiler’s Murine Encephalomyelitis 109
611 Transcriptional profiling of cholesterol biosynthesis 109
612 Changes in the lipid composition of blood serum, liver, and spinal
cord 112
613 Influence of hypercholesterolemia on TMEV-infection 113
614 Conclusion 115
6 2 Application-oriented introduction to microarray analysis 115
6 3 Concluding remarks 116
7 References 119
8 Summary 141
9 Zusammenfassung 143
10 Acknowledgements 145
|
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| isbn | 9783863453206 |
| language | English |
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| spelling | Raddatz, Barbara Beate Rosa Verfasser (DE-588)1122562527 aut Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes submitted by Barbara Beate Rosa Raddatz 1. Auflage Gießen DVG Service GmbH 2016 iv, 145 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Tierärztliche Hochschule Hannover 2016 Germany cabt publication cabt veterinary medicine cabt encephalitis cabt pathology cabt Deutschland (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe Raddatz, Barbara Beate Rosa Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes B:DE-101 application/pdf http://d-nb.info/1122117973/04 Inhaltsverzeichnis HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029597467&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Raddatz, Barbara Beate Rosa Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes Germany cabt publication cabt veterinary medicine cabt encephalitis cabt pathology cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes |
| title_auth | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes |
| title_exact_search | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes |
| title_full | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes submitted by Barbara Beate Rosa Raddatz |
| title_fullStr | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes submitted by Barbara Beate Rosa Raddatz |
| title_full_unstemmed | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes submitted by Barbara Beate Rosa Raddatz |
| title_short | Influence of a dietary-induced hypercholesterolemia on the pathogenesis of Theiler's murine encephalomyelitis with special emphasis on transcriptional changes |
| title_sort | influence of a dietary induced hypercholesterolemia on the pathogenesis of theiler s murine encephalomyelitis with special emphasis on transcriptional changes |
| topic | Germany cabt publication cabt veterinary medicine cabt encephalitis cabt pathology cabt |
| topic_facet | Germany publication veterinary medicine encephalitis pathology Deutschland Hochschulschrift |
| url | http://d-nb.info/1122117973/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029597467&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT raddatzbarbarabeaterosa influenceofadietaryinducedhypercholesterolemiaonthepathogenesisoftheilersmurineencephalomyelitiswithspecialemphasisontranscriptionalchanges |
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