Cytokinesis:
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam
Elsevier, Academic Press
2017
|
| Series: | Methods in cell biology
Volume 137 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xx, 459 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128096734 |
Staff View
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV044035671 | ||
| 003 | DE-604 | ||
| 005 | 20170927 | ||
| 007 | t | ||
| 008 | 170209s2017 a||| |||| 00||| eng d | ||
| 020 | |a 9780128096734 |9 978-0-12-809673-4 | ||
| 035 | |a (OCoLC)973024241 | ||
| 035 | |a (DE-599)BVBBV044035671 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-11 | ||
| 084 | |a WC 5100 |0 (DE-625)148108:13423 |2 rvk | ||
| 084 | |a WG 2100 |0 (DE-625)148513: |2 rvk | ||
| 245 | 1 | 0 | |a Cytokinesis |c edited by Arnaud Echard |
| 264 | 1 | |a Amsterdam |b Elsevier, Academic Press |c 2017 | |
| 300 | |a xx, 459 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v Volume 137 | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zellteilung |0 (DE-588)4190668-8 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Zellteilung |0 (DE-588)4190668-8 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Echard, Arnaud |4 edt | |
| 830 | 0 | |a Methods in cell biology |v Volume 137 |w (DE-604)BV002534878 |9 137 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg - ADAM Catalogue Enrichment |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029442816&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-029442816 | ||
Record in the Search Index
| _version_ | 1804177042255642624 |
|---|---|
| adam_text | Contents
Contributors.........................................................xiii
Preface......:.......................................................xix
CHAPTER 1 Labeling Membrane Domains in Dividing
Echinoderm Eggs..........................................1
M. Bennett, J. Meaders, S. Ruvolo, D. Burgess
1. General Care and Use of Sea Urchins.................... 2
2. Plasma Membrane Labels..................................4
3. Intracellular Labeling..................................6
Acknowledgments........................................ 12
References..............................................12
CHAPTER 2 Dynamics of Sphingomyelin- and
Cholesterol-Enriched Lipid Domains During
Cytokinesis........................................... 15
M, Abe, T. Kobayashi
Introduction............................................16
1. Methods................................................17
Conclusion..............................................21
Acknowledgment........................................ 22
References..............................................22
CHAPTER 3 Analysis of Protein Dynamics During
Cytokinesis in Budding Yeast............................25
S. Okada, C. Wloka, E. Bi
Introduction............................................26
1. Five-Dimensional Live-Cell Imaging.....................28
2. Photoactivation and Photoconversion....................34
3. Fluorescence Recovery After Photobleaching.............35
Acknowledgments.........................................42
References..............................................42
CHAPTER 4 Imaging Cytokinesis of Drosophila S2 Cells...................47
A. Kechad, G.R.X. Hickson
Introduction.......................................... 48
1. Methods................................................49
Conclusion and Perspectives.............................68
Acknowledgments.........................................68
References..............................................69
Contents
CHAPTER 5 Studying Cytokinesis in Drosophila Epithelial
Tissues................................................73
D. Pinheiro, Y. Bellaiche
Introduction...........................................74
1. Methods and Experimental Setup..........................74
2. Data Processing and Analysis............................78
3. Markers and Genetic Tools to Study Epithelial Cytokinesis
In Vivo..................................................80
Conclusions and Future Perspectives.......................81
Acknowledgments........................................82
References.............................................82
CHAPTER 6 Visualization of Cleavage Furrow Proteins
in Fixed Dividing Spermatocytes..........................85
A. Frappaolo, S. Sechi, G. Belloni, R. Piergentili,
M.G. Giansanti
Introduction: Overview of Cytokinesis in Drosophila
Spermatocytes..........................................86
1 „ Fixation Protocols.................................... 93
2. Staining Protocols....................................97
Acknowledgments........................................100
References..............................................100
CHAPTER 7 Monitoring Complete and Incomplete Abscission
in the Germ Line Stem Cell Lineage of Drosophila
Ovaries.................................................105
J. Mathieu, J.-R. Huynh
Introduction............................................106
1. Methods............................................... 109
Conclusion..............................................116
Acknowledgments........................................116
References..............................................117
CHAPTER 8 Incomplete Cytokinesis/Binucleation in
Mammals: the Powerful System of Hepatocytes............119
M. Fortier, S. Celton-Morizur, C. Desdouets
Introduction...........................................120
1. Experimental Procedures...............................122
Conclusion......................................... 139
Contents vii
Acknowledgments........................................139
Supplemendary Data.....................................139
References.............................................140
CHAPTER 9 Specific Membrane Dynamics During Neural
Stem Cell Division.....................................143
A. Ettinger, Y. Kosodo, W.B. Huttner
Introduction......................................... 144
1. General Considerations.................................146
2. Isolation and Detection of Released Postabscission
Midbodies..............................................151
3. Live-Cell Imaging......................................157
4. High-Voltage Electron Microscopy.......................164
Summary.............................................. 168
Acknowledgments........................................169
References.............................................169
CHAPTER 10 Understanding Post-Mitotic Roles of the
Midbody During Cell Differentiation and
Polarization...........................................173
E. Peterman, R. Prekeris
Introduction......................................... 174
1. Midbody and Epithelial Cell Polarity...................176
2. Analysis of Midbody Uptake by Interphase Cells.........181
Summary................................................185
Acknowledgments........................................185
References.............................................185
CHAPTER 11 Micromanipulation of Daughter Cells for
the Study of Cytokinetic Abscission....................187
J. Lafaurie-Janvore, C. Lafaurie, M. Piel
■ i- Introduction...........................................188
t. Adhesive Micropattems..................................189
2. Laser Ablation of the Intercellular Bridge.............199
Acknowledgments........................................202
References.............................................202
Contents
CHAPTER 12 Measuring Abscission Spatiotemporal
Dynamics Using Quantitative High-Resolution
Microscopy..............................................205
0. Gershony, S. Sherman, S. Adar, I. Segal, D. Nachmias,
1. Golîand, N. Elia
Introduction............................................206
1. Methods................................................212
References..............................................223
CHAPTER 13 Analysis of Cytokinesis by Electron Microscopy....225
J. König, i. Borrego-Pinto, D. Streichen, M. Munzig,
P. Lenart, T. Müller-Reichen
Introduction............................................226
1. Methods................................................227
2. Instrumentation and Materials..........................232
3. Discussion.............................................234
Acknowledgments.........................................236
References..............................................236
CHAPTER 14 Studying Cytokinesis and Midbody Remnants
Using Correlative Light/Scanning EM.....................239
S. Frémont, A. Echard
Introduction............................................240
1. Experimental Procedures................................241
Conclusion..............................................248
Acknowledgments.........................................249
References..............................................249
CHAPTER 15 Analysis of Postcytokinetic Roles of Cytokinetic
Components in Caenorhabditis elegans....................253
Y. Chai, D. Tian, W. Li, G. Ou
Introduction.......................................... 254
1. Generation of Transgenic Caenorhabditis elegans
Expressing Fluorescent Cytokinetic Proteins.............256
2. Creation of Conditional Mutations of Cytokinetic Genes in
Caenorhabditis elegans Using the Somatic CRISPR-Cas9
Technique...............................................258
3. Imaging of Cytokinesis and Postcytokinetic Events in
Caenorhabditis elegans Larvae Using Spinning Disk
Confocal Microscopy.....................................260
Contents
IX
Conclusion............................................262
Acknowledgments.......................................265
References............................................265
CHAPTER 16 Variations on a Theme: Imaging Cytokinetic
and Stable Rings in situ Using Caenorhabditis
elegans...............................................267
K. Rehain, R.A. Green, K.G. Bourdages, A.S. Maddox
Introduction..........................................268
1. Methods...............................................272
Conclusions...........................................278
Acknowledgments.......................................278
Supplementary data....................................279
References............................................279
CHAPTER 17 Using Fast-Acting Temperature-Sensitive Mutants
to Study Cell Division in Caenorhabditis elegans...283
T. Davies, S. Sundaramoorthy, S.N. Jordan,
M. Shirasu-Hiza, J. Dumont, J.C. Canman
Introduction..........................................284
1. Microscopy and Embryo Isolation.......................287
2. Using Fast-Acting Temperature Sensitive Mutant
Embryos to Study Cytokinesis..........................291
3. Characterizing Temperature Sensitive Alleles..........300
4. Choosing a Temperature Control Method.................302
Conclusions...........................................303
Acknowledgments.......................................304
References............................................304
CHAPTER 18 Cytokinesis From Nanometers to Micrometers
and Microseconds to Minutes...........................307
P. Kothari, E.S. Schiffhauer, D.N. Robinson
Introduction..........................................308
1. Micropipette Aspiration...............................309
2. Fluorescence Correlation Spectroscopy.................313
3. Fluorescence Cross-Correlation Spectroscopy...........316
4. Fluorescence Recovery After Photobleaching............318
Conclusion............................................320
Acknowledgments.......................................320
References............................................320
Contents
CHAPTER 19 An Active Contour imageJ Plugin to Monitor
Daughter Cell Size in 3D During Cytokinesis..........323
M. B. Smith, A. Chaigne, E.K. Paluch
1. Background...........................................324
2. Methods..,..........................................328
Conclusion...........................................336
Acknowledgments..................................... 337
Appendix............................................ 337
References.......................................... 339
CHAPTER 20 Nuclear Displacement and Fluorescence
Recovery After Photobleaching (FRAP) Assays
to Study Division Site Placement and
Cytokinesis in Fission Yeast .......................341
P. Ullal, P. Bhatia, S.G. Martin
Introduction........................................ 342
1. Methods............................................. 343
Conclusion.......................................... 350
Acknowledgments..................................... 352
References.......................................... 352
CHAPTER 21 Single-Molecule Measurements to Study
Polymerization Dynamics of FtsZ-FtsA
Copolymers..........................................................355
N. Baranova, M. Loose
Introduction: The Bacterial Cytoskeleton.............356
1. Protein Biochemistry: Expression,. Purification,
and Fluorescent Labeling.............................358
2. Fluorescence Imaging of Protein Dynamics.............362
3. Data Analysis........................................364
Conclusion.......................................... 366
Acknowledgments................................... 366
References.......................................... 367
CHAPTER 22 Preparation of Centraispindlin as an Active
Heterotetramer of Kinesin and GTPase Activating
Protein Subunits for In Vitro Structural and
Functional Assays....................................371
M. Mishima
Introduction........................................ 372
Contents
xi
1. Purification of a Recombinant Caenorhabditis elegans
Centralspindlin Complex Reconstituted in Bacterial
Extract.................................................374
2. Purification of Human Centralspindlin From HeLa Cells..378
Conclusions.............................................383
Acknowledgments.........................................383
References..............................................384
CHAPTER 23 In Vitro Reactivation of the Cytokinetic
Contractile Ring of Fission Yeast Cells.................387
I. Mabuchi, J. Kashiwazaki, M. Mishra
Introduction............................................388
1. Methods.................................................389
Conclusion..............................................393
Acknowledgments.........................................394
References..............................................394
CHAPTER 24 Xenopus Extract Approaches to Studying
Microtubule Organization and Signaling in
Cytokinesis.............................................395
C.M. Field, J.F. Pelletier, T.i. Mitchison
Introduction............................................397
1. Actin-Intact Extract Preparation........................398
2. Fluorescent Probes......................................407
3. Microtubule Organizing Centers and Microtubule
Nucléation..............................................410
4. Preparation of Glass Surfaces for Imaging...............416
5. Protein Immunodepletion.................................419
6. Supported Lipid Bilayers................................421
7. Droplet Microfluidics...................................430
Acknowledgments.........................................433
References..............................................433
Index.................................................................437
Volumes in Series.....................................................447
|
| any_adam_object | 1 |
| author2 | Echard, Arnaud |
| author2_role | edt |
| author2_variant | a e ae |
| author_facet | Echard, Arnaud |
| building | Verbundindex |
| bvnumber | BV044035671 |
| classification_rvk | WC 5100 WG 2100 |
| ctrlnum | (OCoLC)973024241 (DE-599)BVBBV044035671 |
| discipline | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01471nam a2200373 cb4500</leader><controlfield tag="001">BV044035671</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20170927 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">170209s2017 a||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780128096734</subfield><subfield code="9">978-0-12-809673-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)973024241</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044035671</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-11</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5100</subfield><subfield code="0">(DE-625)148108:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 2100</subfield><subfield code="0">(DE-625)148513:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cytokinesis</subfield><subfield code="c">edited by Arnaud Echard</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam</subfield><subfield code="b">Elsevier, Academic Press</subfield><subfield code="c">2017</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">xx, 459 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in cell biology</subfield><subfield code="v">Volume 137</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zellteilung</subfield><subfield code="0">(DE-588)4190668-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Zellteilung</subfield><subfield code="0">(DE-588)4190668-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Echard, Arnaud</subfield><subfield code="4">edt</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in cell biology</subfield><subfield code="v">Volume 137</subfield><subfield code="w">(DE-604)BV002534878</subfield><subfield code="9">137</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg - ADAM Catalogue Enrichment</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029442816&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-029442816</subfield></datafield></record></collection> |
| id | DE-604.BV044035671 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:41:47Z |
| institution | BVB |
| isbn | 9780128096734 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029442816 |
| oclc_num | 973024241 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-11 |
| physical | xx, 459 Seiten Illustrationen, Diagramme |
| publishDate | 2017 |
| publishDateSearch | 2017 |
| publishDateSort | 2017 |
| publisher | Elsevier, Academic Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Cytokinesis edited by Arnaud Echard Amsterdam Elsevier, Academic Press 2017 xx, 459 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 137 Methode (DE-588)4038971-6 gnd rswk-swf Zellteilung (DE-588)4190668-8 gnd rswk-swf Zellteilung (DE-588)4190668-8 s Methode (DE-588)4038971-6 s b DE-604 Echard, Arnaud edt Methods in cell biology Volume 137 (DE-604)BV002534878 137 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029442816&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cytokinesis Methods in cell biology Methode (DE-588)4038971-6 gnd Zellteilung (DE-588)4190668-8 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4190668-8 |
| title | Cytokinesis |
| title_auth | Cytokinesis |
| title_exact_search | Cytokinesis |
| title_full | Cytokinesis edited by Arnaud Echard |
| title_fullStr | Cytokinesis edited by Arnaud Echard |
| title_full_unstemmed | Cytokinesis edited by Arnaud Echard |
| title_short | Cytokinesis |
| title_sort | cytokinesis |
| topic | Methode (DE-588)4038971-6 gnd Zellteilung (DE-588)4190668-8 gnd |
| topic_facet | Methode Zellteilung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029442816&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT echardarnaud cytokinesis |