Cytokinesis:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Elsevier, Academic Press
2017
|
| Schriftenreihe: | Methods in cell biology
Volume 137 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xx, 459 Seiten Illustrationen, Diagramme |
| ISBN: | 9780128096734 |
Internformat
MARC
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| 300 | |a xx, 459 Seiten |b Illustrationen, Diagramme | ||
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Datensatz im Suchindex
| _version_ | 1804177042255642624 |
|---|---|
| adam_text | Contents
Contributors.........................................................xiii
Preface......:.......................................................xix
CHAPTER 1 Labeling Membrane Domains in Dividing
Echinoderm Eggs..........................................1
M. Bennett, J. Meaders, S. Ruvolo, D. Burgess
1. General Care and Use of Sea Urchins.................... 2
2. Plasma Membrane Labels..................................4
3. Intracellular Labeling..................................6
Acknowledgments........................................ 12
References..............................................12
CHAPTER 2 Dynamics of Sphingomyelin- and
Cholesterol-Enriched Lipid Domains During
Cytokinesis........................................... 15
M, Abe, T. Kobayashi
Introduction............................................16
1. Methods................................................17
Conclusion..............................................21
Acknowledgment........................................ 22
References..............................................22
CHAPTER 3 Analysis of Protein Dynamics During
Cytokinesis in Budding Yeast............................25
S. Okada, C. Wloka, E. Bi
Introduction............................................26
1. Five-Dimensional Live-Cell Imaging.....................28
2. Photoactivation and Photoconversion....................34
3. Fluorescence Recovery After Photobleaching.............35
Acknowledgments.........................................42
References..............................................42
CHAPTER 4 Imaging Cytokinesis of Drosophila S2 Cells...................47
A. Kechad, G.R.X. Hickson
Introduction.......................................... 48
1. Methods................................................49
Conclusion and Perspectives.............................68
Acknowledgments.........................................68
References..............................................69
Contents
CHAPTER 5 Studying Cytokinesis in Drosophila Epithelial
Tissues................................................73
D. Pinheiro, Y. Bellaiche
Introduction...........................................74
1. Methods and Experimental Setup..........................74
2. Data Processing and Analysis............................78
3. Markers and Genetic Tools to Study Epithelial Cytokinesis
In Vivo..................................................80
Conclusions and Future Perspectives.......................81
Acknowledgments........................................82
References.............................................82
CHAPTER 6 Visualization of Cleavage Furrow Proteins
in Fixed Dividing Spermatocytes..........................85
A. Frappaolo, S. Sechi, G. Belloni, R. Piergentili,
M.G. Giansanti
Introduction: Overview of Cytokinesis in Drosophila
Spermatocytes..........................................86
1 „ Fixation Protocols.................................... 93
2. Staining Protocols....................................97
Acknowledgments........................................100
References..............................................100
CHAPTER 7 Monitoring Complete and Incomplete Abscission
in the Germ Line Stem Cell Lineage of Drosophila
Ovaries.................................................105
J. Mathieu, J.-R. Huynh
Introduction............................................106
1. Methods............................................... 109
Conclusion..............................................116
Acknowledgments........................................116
References..............................................117
CHAPTER 8 Incomplete Cytokinesis/Binucleation in
Mammals: the Powerful System of Hepatocytes............119
M. Fortier, S. Celton-Morizur, C. Desdouets
Introduction...........................................120
1. Experimental Procedures...............................122
Conclusion......................................... 139
Contents vii
Acknowledgments........................................139
Supplemendary Data.....................................139
References.............................................140
CHAPTER 9 Specific Membrane Dynamics During Neural
Stem Cell Division.....................................143
A. Ettinger, Y. Kosodo, W.B. Huttner
Introduction......................................... 144
1. General Considerations.................................146
2. Isolation and Detection of Released Postabscission
Midbodies..............................................151
3. Live-Cell Imaging......................................157
4. High-Voltage Electron Microscopy.......................164
Summary.............................................. 168
Acknowledgments........................................169
References.............................................169
CHAPTER 10 Understanding Post-Mitotic Roles of the
Midbody During Cell Differentiation and
Polarization...........................................173
E. Peterman, R. Prekeris
Introduction......................................... 174
1. Midbody and Epithelial Cell Polarity...................176
2. Analysis of Midbody Uptake by Interphase Cells.........181
Summary................................................185
Acknowledgments........................................185
References.............................................185
CHAPTER 11 Micromanipulation of Daughter Cells for
the Study of Cytokinetic Abscission....................187
J. Lafaurie-Janvore, C. Lafaurie, M. Piel
■ i- Introduction...........................................188
t. Adhesive Micropattems..................................189
2. Laser Ablation of the Intercellular Bridge.............199
Acknowledgments........................................202
References.............................................202
Contents
CHAPTER 12 Measuring Abscission Spatiotemporal
Dynamics Using Quantitative High-Resolution
Microscopy..............................................205
0. Gershony, S. Sherman, S. Adar, I. Segal, D. Nachmias,
1. Golîand, N. Elia
Introduction............................................206
1. Methods................................................212
References..............................................223
CHAPTER 13 Analysis of Cytokinesis by Electron Microscopy....225
J. König, i. Borrego-Pinto, D. Streichen, M. Munzig,
P. Lenart, T. Müller-Reichen
Introduction............................................226
1. Methods................................................227
2. Instrumentation and Materials..........................232
3. Discussion.............................................234
Acknowledgments.........................................236
References..............................................236
CHAPTER 14 Studying Cytokinesis and Midbody Remnants
Using Correlative Light/Scanning EM.....................239
S. Frémont, A. Echard
Introduction............................................240
1. Experimental Procedures................................241
Conclusion..............................................248
Acknowledgments.........................................249
References..............................................249
CHAPTER 15 Analysis of Postcytokinetic Roles of Cytokinetic
Components in Caenorhabditis elegans....................253
Y. Chai, D. Tian, W. Li, G. Ou
Introduction.......................................... 254
1. Generation of Transgenic Caenorhabditis elegans
Expressing Fluorescent Cytokinetic Proteins.............256
2. Creation of Conditional Mutations of Cytokinetic Genes in
Caenorhabditis elegans Using the Somatic CRISPR-Cas9
Technique...............................................258
3. Imaging of Cytokinesis and Postcytokinetic Events in
Caenorhabditis elegans Larvae Using Spinning Disk
Confocal Microscopy.....................................260
Contents
IX
Conclusion............................................262
Acknowledgments.......................................265
References............................................265
CHAPTER 16 Variations on a Theme: Imaging Cytokinetic
and Stable Rings in situ Using Caenorhabditis
elegans...............................................267
K. Rehain, R.A. Green, K.G. Bourdages, A.S. Maddox
Introduction..........................................268
1. Methods...............................................272
Conclusions...........................................278
Acknowledgments.......................................278
Supplementary data....................................279
References............................................279
CHAPTER 17 Using Fast-Acting Temperature-Sensitive Mutants
to Study Cell Division in Caenorhabditis elegans...283
T. Davies, S. Sundaramoorthy, S.N. Jordan,
M. Shirasu-Hiza, J. Dumont, J.C. Canman
Introduction..........................................284
1. Microscopy and Embryo Isolation.......................287
2. Using Fast-Acting Temperature Sensitive Mutant
Embryos to Study Cytokinesis..........................291
3. Characterizing Temperature Sensitive Alleles..........300
4. Choosing a Temperature Control Method.................302
Conclusions...........................................303
Acknowledgments.......................................304
References............................................304
CHAPTER 18 Cytokinesis From Nanometers to Micrometers
and Microseconds to Minutes...........................307
P. Kothari, E.S. Schiffhauer, D.N. Robinson
Introduction..........................................308
1. Micropipette Aspiration...............................309
2. Fluorescence Correlation Spectroscopy.................313
3. Fluorescence Cross-Correlation Spectroscopy...........316
4. Fluorescence Recovery After Photobleaching............318
Conclusion............................................320
Acknowledgments.......................................320
References............................................320
Contents
CHAPTER 19 An Active Contour imageJ Plugin to Monitor
Daughter Cell Size in 3D During Cytokinesis..........323
M. B. Smith, A. Chaigne, E.K. Paluch
1. Background...........................................324
2. Methods..,..........................................328
Conclusion...........................................336
Acknowledgments..................................... 337
Appendix............................................ 337
References.......................................... 339
CHAPTER 20 Nuclear Displacement and Fluorescence
Recovery After Photobleaching (FRAP) Assays
to Study Division Site Placement and
Cytokinesis in Fission Yeast .......................341
P. Ullal, P. Bhatia, S.G. Martin
Introduction........................................ 342
1. Methods............................................. 343
Conclusion.......................................... 350
Acknowledgments..................................... 352
References.......................................... 352
CHAPTER 21 Single-Molecule Measurements to Study
Polymerization Dynamics of FtsZ-FtsA
Copolymers..........................................................355
N. Baranova, M. Loose
Introduction: The Bacterial Cytoskeleton.............356
1. Protein Biochemistry: Expression,. Purification,
and Fluorescent Labeling.............................358
2. Fluorescence Imaging of Protein Dynamics.............362
3. Data Analysis........................................364
Conclusion.......................................... 366
Acknowledgments................................... 366
References.......................................... 367
CHAPTER 22 Preparation of Centraispindlin as an Active
Heterotetramer of Kinesin and GTPase Activating
Protein Subunits for In Vitro Structural and
Functional Assays....................................371
M. Mishima
Introduction........................................ 372
Contents
xi
1. Purification of a Recombinant Caenorhabditis elegans
Centralspindlin Complex Reconstituted in Bacterial
Extract.................................................374
2. Purification of Human Centralspindlin From HeLa Cells..378
Conclusions.............................................383
Acknowledgments.........................................383
References..............................................384
CHAPTER 23 In Vitro Reactivation of the Cytokinetic
Contractile Ring of Fission Yeast Cells.................387
I. Mabuchi, J. Kashiwazaki, M. Mishra
Introduction............................................388
1. Methods.................................................389
Conclusion..............................................393
Acknowledgments.........................................394
References..............................................394
CHAPTER 24 Xenopus Extract Approaches to Studying
Microtubule Organization and Signaling in
Cytokinesis.............................................395
C.M. Field, J.F. Pelletier, T.i. Mitchison
Introduction............................................397
1. Actin-Intact Extract Preparation........................398
2. Fluorescent Probes......................................407
3. Microtubule Organizing Centers and Microtubule
Nucléation..............................................410
4. Preparation of Glass Surfaces for Imaging...............416
5. Protein Immunodepletion.................................419
6. Supported Lipid Bilayers................................421
7. Droplet Microfluidics...................................430
Acknowledgments.........................................433
References..............................................433
Index.................................................................437
Volumes in Series.....................................................447
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| indexdate | 2024-07-10T07:41:47Z |
| institution | BVB |
| isbn | 9780128096734 |
| language | English |
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| spelling | Cytokinesis edited by Arnaud Echard Amsterdam Elsevier, Academic Press 2017 xx, 459 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 137 Methode (DE-588)4038971-6 gnd rswk-swf Zellteilung (DE-588)4190668-8 gnd rswk-swf Zellteilung (DE-588)4190668-8 s Methode (DE-588)4038971-6 s b DE-604 Echard, Arnaud edt Methods in cell biology Volume 137 (DE-604)BV002534878 137 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029442816&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cytokinesis Methods in cell biology Methode (DE-588)4038971-6 gnd Zellteilung (DE-588)4190668-8 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4190668-8 |
| title | Cytokinesis |
| title_auth | Cytokinesis |
| title_exact_search | Cytokinesis |
| title_full | Cytokinesis edited by Arnaud Echard |
| title_fullStr | Cytokinesis edited by Arnaud Echard |
| title_full_unstemmed | Cytokinesis edited by Arnaud Echard |
| title_short | Cytokinesis |
| title_sort | cytokinesis |
| topic | Methode (DE-588)4038971-6 gnd Zellteilung (DE-588)4190668-8 gnd |
| topic_facet | Methode Zellteilung |
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