Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease: Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English German |
| Published: |
Erlangen ; Nürnberg
2016
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 201 Seiten Illustrationen, Diagramme |
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| 100 | 1 | |a Heußner, Kirsten |e Verfasser |0 (DE-588)1123158142 |4 aut | |
| 245 | 1 | 0 | |a Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease |b Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS |c vorgelegt von Kirsten Heußner, geboren in Frankfurt/Main |
| 246 | 1 | 1 | |a Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung |
| 264 | 1 | |a Erlangen ; Nürnberg |c 2016 | |
| 300 | |a 201 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2016 | ||
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Record in the Search Index
| _version_ | 1804176969381707776 |
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| adam_text | CHARACTERIZATION OF 11 -BEFA-HYDROXYSTEROID
DEHYDROGENASE ACTIVITY IN PLACENTA
FUNCTION AND INFLAMMATORY BOWEL DISEASE -
INVESTIGATIONS INTO STEROID METABOLISM
BY LC-MS/MS
CHARAKTERISIERUNG DER 11 -OEEFA-HYDROXYSTEROID-
DEHYDROGENASEAKTIVITAET IN PLAZENTAGEWEBE UND BEI
ENTZUENDLICHER DARMERKRANKUNG - UNTERSUCHUNGEN DES
STEROIDMETABOLISMUS MITTELS LC-MS/MS
DISSERTATION
ZUR ERLANGUNG DES AKADEMISCHEN GRADES
DOCTOR RERUM NATURALIUM (DR. RER. NAT.)
VORGELEGT DER
NATURWISSENSCHAFTLICHEN FAKULTAET
DER FRIEDRICH-ALEXANDER-UNIVERSITAET ERLANGEN-NUERNBERG
IN KOOPERATION MIT DER
KINDER- UND JUGENDKLINIK
DES UNIVERSITAETSKLINIKUMS ERLANGEN
VON KIRSTEN HEUSSNER
GEBOREN IN FRANKFURT/MAIN
TABLE OF CONTENTS
S
UMMARY
........................................................................................................................1
Z
USAMMENFASSUNG
.......................................................................................................4
PROLOGUE............................................................................................................................
7
1 IN T R O D U C T IO N
..................................................................................................................
10
1.1
G
LUCOCORTICOIDS
..........................................................................................................
10
1.1.1
BIOSYNTHESIS...............................................................................................................11
1.1.2
REGULATION..................................................................................................................
14
1.1.3 CORTICOSTERONE AS
GLUCOCORTICOID..........................................................................15
1.2
B
ILE
A
CID S
.........................................................................................................................16
1.2.1
CLASSIFICATION.............................................................................................................
16
1.2.2
BIOSYNTHESIS...............................................................................................................17
1.2.3
PHYSIOLOGY..................................................................................................................
20
1.3
C
ORTICOTROPIN
R
ELEASING
H
ORMONE
......................................................................
21
1.3.1
BIOSYNTHESIS..............................................................................................................
21
1.3.2
PHYSIOLOGY..................................................................................................................
22
1.3.3 SPECIES-SPECIFIC DIFFERENCES IN THE CRH PROTEIN S EQ U EN
CE.......................24
1.4 G
LUCOCORTICOID
R
EGULATION
AND
11 SS-HSD E
NZYMOLOGY
...........................
24
1.4.1 GLUCOCORTICOID AND MINERALOCORTICOID
RECEPTORS..............................................24
1.4.2 REACTION DIRECTION AND
COSUBSTRATES.................................................................
26
1.4.3
DISTRIBUTION.................................................................................................................27
1.4.4 MEASUREMENT OF 11 SS-HSD ACTIVITY
......................................................................
28
1.5 P
ATHOPHYSIOLOGY
.............................................................................................
29
1.5.1 GLUCOCORTICOID METABOLISM IN PLACENTAL BARRIER
FUNCTION...............................31
1.5.2 GLUCOCORTICOID METABOLISM IN THE GASTROINTESTINAL T RA C
T................................32
1.5.2.1 PREVALENCE AND INCIDENCE OF CHRONIC INFLAMMATORY BOWEL
D
ISEASE............................................................................................................
32
1.5.2.2 GLUCOCORTICOID METABOLISM IN INFLAMMATORY BOWEL D ISE ASE
...............
33
1.5.2.3 INFLUENCE OF BILE ACIDS ON THE GASTROINTESTINAL T RA C T
...........................
34
2 O B J E C T IV E S
.........................................................................................................................36
2.1 G
L U C O C O R TIC O ID
M
ETABO LISM
IN
P
LA C E N T A
.............................................................
37
2.2 G
L U C O C O R TIC O ID
M
ETABOLISM
IN
C
H R O N IC
I
NFLA M M A TO R Y
B
O W EL
D
IS E A S E
..............................................................................................................................
38
3 EXPERIM ENTAL S E C T IO N
..............................................................................................
40
3.1 M
A T E R IA L S
.........................................................................................................................
40
3.1.1 GENERALLY USED
MATERIALS.......................................................................................
40
3.1.1.1 CONSUMABLE M
ATERIALS...................................................................................40
3.1.1.2 CHEMICALS AND
REAGENTS..............................................................................
41
3.1.2 PREPARED BUFFERS AND
SOLUTIONS............................................................................
42
3.1.2.1 BUFFERS AND SOLUTIONS FOR CELL CULTURE
EXPERIMENTS................................43
3.1.2.2 SOLUTIONS FOR 11 SS-HSD ACTIVITY ASSAYS
......................................................
45
3.1.3 BIOLOGICAL M
ATERIALS.................................................................................................
46
3.1.4 CELL
LINES....................................................................................................................46
3.1.5 CALIBRATORS AND INTERNAL
STANDARDS......................................................................
47
3.1.6
EQUIPMENT...................................................................................................................48
3.1.7
SOFTWARE......................................................................................................................
50
3.2 E
N ZYM E
C
H A R A C T E R IZ A T IO N
..........................................................................................
51
3.2.1 MEASUREMENT OF ENZYME
ACTIVITIY........................................................................51
3.2.1.1 PREPARATION OF THE MICROSOMAL
FRACTION.................................................... 52
3.2.1.2 PROTEIN A S S A Y
...................................................................................................52
3.2.1.3 GENERAL PROCEDURE FOR ENZYME A SSAYS
...................................................
53
3.2.1.4 PARTICULAR REACTION CONDITIONS FOR ENZYME ASSAYS
................................
53
3.2.2 RECOMBINANT HUMAN 11 SS-HSD ENZYMES
..........................................................
54
3.2.2.1 GENERATION OF RECOMBINANT HUMAN 11SS-HSD1 PROTEIN VIA
BACULOVIRAL GENE
TRANSFER...........................................................................
54
3.2.2 2 GENERATION OF RECOMBINANT HUMAN 11SS-HSD2 PROTEIN VIA GENE
TRANSFER INTO EUKARYOTIC C
ELLS.....................................................................
56
3.2.2 3 11SS-HSD1 OR 11SS-HSD2 PROTEIN DETECTION IN WESTERN B LOT
................
56
3.3 S
T E R O ID
A
N A L Y S IS
...........................................................................................................58
3.3.1 STEROID ANALYSIS VIA LC -M S/M S
............................................................................
58
3.3.2 COMPARISON OF DIFFERENT ADHESIVE BLOOD MICROSAMPLING SYSTEMS
..............
60
3.3.2.1 CORTISOL SUPPLEMENTATION
EXPERIMENT......................................................60
3.3.2 2 CALIBRATION AND VALIDATION S
ETS...................................................................62
3.3.2 3 COMPARISON OF RAT BLOOD SAMPLES WITH REGARD TO AGE AND
G EN
DER.............................................................................................................
63
3.3.3 STEROID ANALYSIS VIA G C -M S /M S
.........................................................................
63
3.4 CRH
P
EPTIDE
A
NALYSIS
VIA
LC -M S /M S
.....................................................................
65
3.5
C
HARACTERIZATION
OF
P
LACENTA
S
AMPLES
.............................................................. 68
3.5.1 ANALYSIS OF SPECIES-SPECIFIC DIFFERENCES
...........................................................
68
3.5.2 ANALYSIS OF THE IMPACT OF THE MODE OF DELIVERY IN THE HUMAN
ORGANISM...68
3.6
B
ILE
A
CID
A
NALYSIS
VIA
L C -M S /M S
.............................................................................71
3.6.1 DEVELOPMENT OF A SCHEDULED MULTIPLE REACTION MONITORING METHOD FOR
BILE ACID
ANALYSIS....................................................................................................71
3.6.2 VALIDATION OF THE DEVELOPED LC-MS/MS METHOD FOR BILE ACID ANALYSIS
.....
73
3.6.3 SAMPLE PREPARATION AND LC-MS/MS
ANALYSIS..................................................75
3.7
S
TUDY
ON
G
LUCOCORTICOID
M
ETABOLISM
IN
C
HRONIC
I
NFLAMMATORY
B
OWEL
D
ISEASE
IN
C
HILDREN
AND
A
DOLESCENTS
.....................................................77
4 R E S U L T S
...............................................................................................................................
79
4.1 11 SS-HSD
A
CTIVITY
A
SSAYS
............................................................................................
79
4.1.1 PORCINE TISSUE
MICROSOMES..................................................................................79
4.1.2 HUMAN LIVER AND PLACENTAL MICROSOMES
...........................................................
81
4.1.3 INHIBITION OF 11SS-HSD1 IN HUMAN LIVER MICROSOM
ES......................................63
4.1.4 RECOMBINANT HUMAN 1 1SS-HSD1 AND 1 1SS-H
SD2.............................................85
4.1.4.1 PRODUCTION OF RECOMBINANT HUMAN 1 1SS -H S D
1......................................85
4.1.4.2 PRODUCTION OF RECOMBINANT HUMAN 11SS-H S D
2......................................86
4.1.4.3 CHARACTERIZATION OF RECOMBINANT HUMAN 11SS-HSD1 AND
1 1 SS -H S D 2
.......................................................................................................
87
4.2 C
OMPARISON
OF
D
IFFERENT
A
DHESIVE
B
LOOD
M
ICROSAMPLING
S
YSTEMS
.......
83
4.2.1 CORTISOL SUPPLEMENTATION IN RAT BLOOD SAMPLES USING ADHESIVE BLOOD
MICROSAMPLING
SYSTEMS.........................................................................................
88
4.2.2 GLUCOCORTICOID ANALYSIS VIA ADHESIVE BLOOD MICROSAMPLING SYSTEM S
.......
89
4.2.3 COMPARATIVE STEROID ANALYSIS IN JUVENILE AND ADULT RAT BLOOD
S AM
PLES....................................................................................................................
92
4.2.4 COMPARATIVE STEROID ANALYSIS IN FETAL AND NEONATAL RAT BLOOD
S AM
PLES....................................................................................................................
93
4.3 C
O M P A R IS O N
OF
S
P E C IE S
-S
P E C IFIC
D
IFF E R E N C E S
OF
H
UMAN
A N D
R
AT
T
ERM
P
LACENTA
T
ISS U E
S
A M P L E S
...............................................................................
95
4.3.1 CRH PEPTIDE
ANALYSIS.............................................................................................
95
4.3.2 GLUCOCORTICOID
ANALYSIS...........................................................................................96
4.3.3 PLACENTAL IN VITRO ACTIVITY ASSAYS OF 11 SS-H S D
...................................................
98
4.3.4 FURTHER STEROID
ANALYSIS.......................................................................................
100
4.4 C
O M P A R IS O N
OF
H
UMAN
P
LACENTAL
T
ISS U E
S
A M P LE S
W IT H
R
EG A R D
TO
T H E
M
O D E
OF
D
E L IV E R Y
................................................................................................
102
4.4.1 ANALYTE
CORRELATIONS...............................................................................................102
4.4.1.1 11 SS-HSD2-CATALYZED CORTISOL DEACTIVATION IN
PLACENTA.......................103
4.4.1.2 11 SS-HSD2-CATALYZED CORTICOSTERONE DEACTIVATION IN PLACENTA
........
105
4.4.1.3 CORTISOL VERSUS CORTICOSTERONE METABOLISM IN PLACENTA
......................
107
4.4.2 INFLUENCE OF THE MODE OF DELIVERY AND CHOICE OF SAMPLING SITE ON
DIRECT AND INDIRECT DETERMINANTS OF PLACENTAL 11SS-HSD2 ACTIVITY
...........
109
4.4.2.1 ANALYSIS OF 11SS-HSD2 ACTIVITY IN RELATION TO THE MODE OF
DELIVERY. 109
4.4.2.2 COMPARISON OF THE DETERMINANTS OF 11SS-HSD2 ACTIVITY IN
DEPENDENCE OF DIFFERENT SAMPLING S
ITES...............................................114
4.4.2.3 CORRELATION T A B LE S
.......................................................................................
122
4.5 B
ILE
A
C ID
A
SS AY
V
A L ID A T IO N
.......................................................................................126
4.6 G
L U C O C O R TIC O ID
M
ETABOLISM
IN
I
N FLA M M A TO R Y
B
O W EL
D
ISE ASE
IN
C
HILD R EN
A N D
A
D O L E S C E N T S
.....................................................................................
129
4.6.1 GLUCOCORTICOID ANALYSIS IN SERUM S AM
PLES....................................................129
4.6.2 GLUCOCORTICOID ANALYSIS IN URINE S AM
PLES....................................................... 131
4.6.2.1 COMPARATIVE GLUCOCORTICOID ANALYSIS BETWEEN 24 H COLLECTION
AND THE FIRST DAILY SPONTANEOUS U RIN E
..................................................
131
4.6.2 2 ANALYSIS OF GLUCOCORTICOID METABOLITES IN URINE SAM PLES
..............
133
4.6.3 BILE ACID ANALYSIS IN SERUM SAM PLES
...............................................................
136
5 D IS C U S S IO N
......................................................................................................................
141
5.1 11 SS-HSD
A
C T IV ITY
A
S S A Y S
..........................................................................................
141
5.1.1 PRODUCTION OF RECOMBINANT HUMAN 11 SS-HSD EN ZYM
ES..............................141
5.1.1.1 PRODUCTION OF RECOMBINANT HUMAN 11SS-H S D
1.....................................141
5.1.1.2 PRODUCTION OF RECOMBINANT HUMAN 11SS-H S D
2.....................................142
5.1.2 11 SS-HSD ACTIVITY ASSAYS ON MICROSOMES AND CELL LYSATES
.........................
142
5.1.2.1 DIFFERENT PORCINE TISSUE MICROSOMES
......................................................
143
5.1.2.2 HUMAN LIVER AND PLACENTAL MICROSOMES
................................................
143
5.1.2.3 INHIBITION OF 11SS-HSD1 IN HUMAN LIVER
MICROSOMES...........................144
5.1.2.4 RECOMBINANT HUMAN 11SS-HSD1 AND 11 SS -H S D 2
.................................145
5.2
V
ALIDATION
OF
A
DHESIVE
B
LOOD
M
ICROSAMPLING
S
YSTEMS
..............................
146
5.2.1 CORTISOL SUPPLEMENTATION OF RAT BLOOD SAMPLES USING ADHESIVE BLOOD
MICROSAMPLING
SYSTEMS......................................................................................146
5.2.2 GLUCOCORTICOID ANALYSIS VIA ADHESIVE BLOOD MICROSAMPLING SYSTEM S
.....
147
5.2.3 COMPARATIVE STEROID ANALYSIS IN JUVENILE AND ADULT RAT BLOOD
SAM
PLES.................................................................................................................
147
5.2.4 COMPARATIVE STEROID ANALYSIS IN FETAL AND NEONATAL RAT BLOOD
S AM
PLES.................................................................................................................
148
5.3
C
OMPARISON
OF
S
PECIES
-S
PECIFIC
D
IFFERENCES
OF
H
UMAN
AND
R
AT
T
ERM
P
LACENTA
T
ISSUE
S
AMPLES
...........................................................................
150
5.4
C
OMPARISON
OF
H
UMAN
T
ERM
P
LACENTAL
T
ISSUE
S
AMPLES
WITH
R
EGARD
TO
THE
M
ODE
OF
D
ELIVERY
........................................................................................
153
5.4.1 11 SS-HSD2-CATALYZED CORTISOL
DEACTIVATION.....................................................154
5.4.1.1 11SS-HSD2 IN PLACENTAL CORTISOL
METABOLISM.........................................154
5.4.1.2 CRH FEEDBACK ON
CORTISOL-METABOLISM................................................157
5.4.2 11 SS-HSD2-CATALYZED CORTICOSTERONE DEACTIVATION
.......................................
159
5.4.2.1 11SS-HSD2 IN PLACENTAL CORTICOSTERONE METABOLISM
............................
159
5.4.2 2 CRH FEEDBACK ON
CORTICOSTERONE-METABOLISM.................................160
5.4.3 CORTISOL VERSUS CORTICOSTERONE METABOLISM IN PLACENTA
..............................
160
5.4.4 ANNOTATIONS TO THE INFLUENCE OF THE MODE OF DELIVERY AND CHOICE OF
SAMPLING SITE ON DIRECT AND INDIRECT DETERMINANTS OF PLACENTAL
11SS-HSD2
ACTIVITY................................................................................................163
5.4.5 SUMMARY AND CONCLUSION
..................................................................................
165
5.5 LC-MS/MS
M
ETHOD
D
EVELOPMENT
FOR
B
ILE
A
CID
P
ROFILING
..........................
167
5.5.1 LC-MS/MS METHOD DEVELOPMENT
.....................................................................
167
5.5.2 COMPARISON OF THE DEVELOPED BILE ACIDS METHOD WITH THE BIOCRATES
BILE ACIDS
METHOD.................................................................................................168
5.6
G
LUCOCORTICOID
M
ETABOLISM
IN
I
NFLAMMATORY
B
OWEL
D
ISEASE
IN
C
HILDREN
AND
A
DOLESCENTS
....................................................................................169
5.6.1 GLUCOCORTICOID ANALYSIS IN SERUM AND URINE SAM PLES
................................
169
5.6.2 COMPARATIVE GLUCOCORTICOID ANALYSIS BETWEEN 24 H COLLECTION URINE
AND THE FIRST SPONTANEOUS MORNING U RIN
E.....................................................171
5.6.3 ANALYSIS OF GLUCOCORTICOID METABOLITES IN URINE S AM
PLES...........................171
5.6.4 BILE ACID PROFILES IN SERUM S AM P
LES............................................................... 172
6 R E F E R E N C E S
....................................................................................................................
176
LIST OF
ABBREVIATIONS.........................................................................................
190
LIST OF
TABLES....................................................................................................195
LIST OF
FIGURES...................................................................................................197
GENERAL
NOTATIONS.............................................................................................199
A
CKNOWLEDGEMENT
................................................................................................................200
|
| any_adam_object | 1 |
| author | Heußner, Kirsten |
| author_GND | (DE-588)1123158142 |
| author_facet | Heußner, Kirsten |
| author_role | aut |
| author_sort | Heußner, Kirsten |
| author_variant | k h kh |
| building | Verbundindex |
| bvnumber | BV043993429 |
| ctrlnum | (OCoLC)969664829 (DE-599)BVBBV043993429 |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV043993429 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:40:37Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029401585 |
| oclc_num | 969664829 |
| open_access_boolean | |
| owner | DE-29 DE-29T DE-12 DE-355 DE-BY-UBR |
| owner_facet | DE-29 DE-29T DE-12 DE-355 DE-BY-UBR |
| physical | 201 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| record_format | marc |
| spelling | Heußner, Kirsten Verfasser (DE-588)1123158142 aut Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS vorgelegt von Kirsten Heußner, geboren in Frankfurt/Main Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung Erlangen ; Nürnberg 2016 201 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Alexander-Universität Erlangen-Nürnberg 2016 Plazenta (DE-588)4174904-2 gnd rswk-swf Hydroxysteroid-Dehydrogenasen (DE-588)4571089-2 gnd rswk-swf LC-MS (DE-588)4539813-6 gnd rswk-swf Darmentzündung (DE-588)4152463-9 gnd rswk-swf Steroidstoffwechsel (DE-588)4183158-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Hydroxysteroid-Dehydrogenasen (DE-588)4571089-2 s Darmentzündung (DE-588)4152463-9 s Steroidstoffwechsel (DE-588)4183158-5 s LC-MS (DE-588)4539813-6 s DE-604 Plazenta (DE-588)4174904-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029401585&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heußner, Kirsten Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS Plazenta (DE-588)4174904-2 gnd Hydroxysteroid-Dehydrogenasen (DE-588)4571089-2 gnd LC-MS (DE-588)4539813-6 gnd Darmentzündung (DE-588)4152463-9 gnd Steroidstoffwechsel (DE-588)4183158-5 gnd |
| subject_GND | (DE-588)4174904-2 (DE-588)4571089-2 (DE-588)4539813-6 (DE-588)4152463-9 (DE-588)4183158-5 (DE-588)4113937-9 |
| title | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS |
| title_alt | Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung |
| title_auth | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS |
| title_exact_search | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS |
| title_full | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS vorgelegt von Kirsten Heußner, geboren in Frankfurt/Main |
| title_fullStr | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS vorgelegt von Kirsten Heußner, geboren in Frankfurt/Main |
| title_full_unstemmed | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS vorgelegt von Kirsten Heußner, geboren in Frankfurt/Main |
| title_short | Characterization of 11-beta-hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease |
| title_sort | characterization of 11 beta hydroxysteroid dehydrogenase activity in placenta function and inflammatory bowel disease investigations into steroid metabolism by lc ms ms charakterisierung der 11 beta hydroxysteroid dehydrogenaseaktivitat in plazentagewebe und bei entzundlicher darmerkrankung untersuchungen des steroidmetabolismus mittels lc ms ms |
| title_sub | Investigations into steroid metabolism by LC-MS/MS = Charakterisierung der 11-beta-Hydroxysteroid-dehydrogenaseaktivität in Plazentagewebe und bei entzündlicher Darmerkrankung : Untersuchungen des Steroidmetabolismus mittels LC-MS/MS |
| topic | Plazenta (DE-588)4174904-2 gnd Hydroxysteroid-Dehydrogenasen (DE-588)4571089-2 gnd LC-MS (DE-588)4539813-6 gnd Darmentzündung (DE-588)4152463-9 gnd Steroidstoffwechsel (DE-588)4183158-5 gnd |
| topic_facet | Plazenta Hydroxysteroid-Dehydrogenasen LC-MS Darmentzündung Steroidstoffwechsel Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029401585&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT heußnerkirsten characterizationof11betahydroxysteroiddehydrogenaseactivityinplacentafunctionandinflammatoryboweldiseaseinvestigationsintosteroidmetabolismbylcmsmscharakterisierungder11betahydroxysteroiddehydrogenaseaktivitatinplazentagewebeundbeientzundlicherdarmerkrank AT heußnerkirsten charakterisierungder11betahydroxysteroiddehydrogenaseaktivitatinplazentagewebeundbeientzundlicherdarmerkrankung |