Imaging bacterial molecules, structures and cells:
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Elsevier, AP
2016
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in microbiology
volume 43 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xi, 143 Seiten Illustrationen |
| ISBN: | 9780128093924 |
Internformat
MARC
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Datensatz im Suchindex
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| adam_text | Contents
Contributors...............................................................ix
Preface....................................................................xi
SECTION 1 FLUORESCENT MICROSCOPY__________________________________________
CHAPTER 1 Methods for Visualization of Peptidoglycan
Biosynthesis..............................................3
Y.-P. Hsu, X. Meng, M.S. VanNieuwenhze
1. Peptidoglycan Structure and Biosynthesis..................4
1.1. Peptidoglycan Structure and Configuration............4
1.2. Enzymatic Pathway of Peptidoglycan Biosynthesis......6
1.3. Structural Variations in Peptidoglycan Structure.....7
2. Strategies for Peptidoglycan Imaging: Electron Microscopy..9
2.1. Revealing Bacterial Morphology.......................9
2.2. Elucidating PG Structure via EM.....................11
2.3. Cryo-TEM: Studying Cell Wall Organization
in Natural State....................................13
2.4. Three-Dimensional EM Technique: Cryoelectron
Tomography..........................................14
2.5. More Tools: AFM in Bacterial Cell Wall Studies......16
3. Strategies of Peptidoglycan Imaging: Optical
Microscopy...............................................18
3.1. Optical Microscopy Operation and Application........18
3.2. Fluorescent Molecular Probes for PG-Specific
Labelling...........................................21
3.3. Development of Metabolic Probes for PG-Specific
Labelling: FDAAs....................................24
4. Applications of FDAAs and Their Derivatives.............34
4.1. Tools for Studies of PG Synthesis Pattern and
Dynamics of PG......................................34
4.2. PG Recycling/Remodelling in Streptococcus
pneumoniae..........................................36
4.3. FDAAs Reveal PG in Strains not Previously Known
to Possess It.......................................38
Conclusions..............................................41
References...............................................42
V
vi
Contents
CHAPTER 2 Time-Lapse Microscopy and Image Analysis
of Escherichia coli Cells in Mother Machines...............49
Y. Yang, X. Song, A.B. Lindner
1. Introduction...............................................49
2. Experimental Designs.......................................50
2.1. General Principles of the Mother Machine..............50
2.2. Device Design Considerations..........................51
2.3. Time-Lapse Considerations.............................53
3. Experimental Procedures....................................54
3.1. Making Polydimethylsiloxane Mother Machine
Devices................................................55
3.2. Setting up the Fluidic System.........................55
3.3. Cell Culture and Loading..............................56
3.4. Microfluidic and Time-Lapse Setup.....................57
4. Image Analysis for Lineage Construction and
Single-cell Traits.........................................57
4.1. Preprocessing.........................................57
4.2. Segmentation Approaches...............................58
4.3. Lineage Approaches....................................61
4.4. Image Analysis Performances and Results...............64
4.5. Image Analysis Summary................................66
Acknowledgment.............................................66
References.................................................66
CHAPTER 3 Microfluidics for Bacterial Imaging..............................69
L.E. Eland, A. Wipat, S. Lee, S. Park, L.J. Wu
1. Introduction...............................................69
2. Fabrication of Microfluidic Devices.......................71
2.1. Design................................................72
2.2. Production of Silicon Wafers..........................74
2.3. Production of the Microfluidics Chip..................77
3. Fluid Flow................................................77
4. Additional Considerations When Designing and
Setting Up a Microfluidics System........................ 79
5. Computational Analysis and Control of Bacterial
Microfluidic Systems.......................................80
6. Microfluidics for the Engineering of Bacterial Systems....83
7. Biofilm, Microbial Ecology and species-species
Interactions...............................................86
7.1. Multispecies Biofilm Microfluidics....................87
7.2. Spatial Arrangements and Interactions.................90
Contents
8. Cell Cycle Analysis and Size Homeostasis Studies.....90
9. Cell Shape and Geometry Study........................94
10. Future Perspectives..................................96
11. Methods..............................................97
Acknowledgements....................................105
References..........................................105
SECTION 2 ELECTRON MICROSCOPY________________________________________
CHAPTER 4 Electron Cryotomography.................................115
C.M. Oikonomou, M.T. Swulius, A. Briegel, M. Beeby,
Q. Yao, GJ. Jensen
1. Introduction to Electron Cryotomography Technology..115
2. Applications of ECT to Microbiology.................116
3. Comparison to Other Techniques......................120
3.1. Limitations of ECT.............................120
3.2. Advantages of ECT..............................129
Conclusions and Future Directions...................133
Acknowledgements....................................133
References..........................................133
Index
141
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| series | Methods in microbiology |
| series2 | Methods in microbiology |
| spelling | Imaging bacterial molecules, structures and cells Edited by Colin Harwood, Grant J. Jensen First edition Amsterdam Elsevier, AP 2016 xi, 143 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in microbiology volume 43 Bakterien (DE-588)4004296-0 gnd rswk-swf Zelle (DE-588)4067537-3 gnd rswk-swf Bildgebendes Verfahren (DE-588)4006617-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Bakterien (DE-588)4004296-0 s Zelle (DE-588)4067537-3 s Bildgebendes Verfahren (DE-588)4006617-4 s b DE-604 Harwood, Colin R. 1948- (DE-588)108916923X edt Jensen, Grant J. edt Methods in microbiology volume 43 (DE-604)BV000899780 43 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029391972&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Imaging bacterial molecules, structures and cells Methods in microbiology Bakterien (DE-588)4004296-0 gnd Zelle (DE-588)4067537-3 gnd Bildgebendes Verfahren (DE-588)4006617-4 gnd |
| subject_GND | (DE-588)4004296-0 (DE-588)4067537-3 (DE-588)4006617-4 (DE-588)4143413-4 |
| title | Imaging bacterial molecules, structures and cells |
| title_auth | Imaging bacterial molecules, structures and cells |
| title_exact_search | Imaging bacterial molecules, structures and cells |
| title_full | Imaging bacterial molecules, structures and cells Edited by Colin Harwood, Grant J. Jensen |
| title_fullStr | Imaging bacterial molecules, structures and cells Edited by Colin Harwood, Grant J. Jensen |
| title_full_unstemmed | Imaging bacterial molecules, structures and cells Edited by Colin Harwood, Grant J. Jensen |
| title_short | Imaging bacterial molecules, structures and cells |
| title_sort | imaging bacterial molecules structures and cells |
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| topic_facet | Bakterien Zelle Bildgebendes Verfahren Aufsatzsammlung |
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