Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
2016
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| Subjects: | |
| Online Access: | Abstract Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | 145 Seiten Illustrationen, Diagramme |
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|---|---|---|---|
| 001 | BV043874998 | ||
| 003 | DE-604 | ||
| 005 | 20170321 | ||
| 007 | t| | ||
| 008 | 161114s2016 xx a||| m||| 00||| eng d | ||
| 035 | |a (OCoLC)978272960 | ||
| 035 | |a (DE-599)BVBBV043874998 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-83 | ||
| 100 | 1 | |a Wolf, Ingrid |e Verfasser |0 (DE-588)1128184036 |4 aut | |
| 245 | 1 | 0 | |a Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells |c vorgelegt von Dipl.-Ing. Ingrid Wolf, geb. in Shanatas, Kasachstan |
| 264 | 1 | |a Berlin |c 2016 | |
| 300 | |a 145 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Technische Universität Berlin |d 2016 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | |u http://services.ub.tu-berlin.de/abstracts/diss/wolf_ingrid_abstract.pdf |3 Abstract | |
| 856 | 4 | |u http://services.ub.tu-berlin.de/abstracts/diss/wolf_ingrid_inhalt.pdf |3 Inhaltsverzeichnis | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029284755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-029284755 | |
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| _version_ | 1823788694948020224 |
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| adam_text |
TABLE OF CONTENTS
1
ABSTRACT.
6
1.1
ZUSAMMENFASSUNG.
7
2
INTRODUCTION.
10
2.1 THE EARLY B-CELL
DEVELOPMENT.10
2.2 LATE B-CELL DEVELOPMENT IN THE
PERIPHERY.
12
2.2.1 THE BL-CELL
COMPARTMENT.13
2.2.2 THE CONVENTIONAL B2-CELL
COMPARTMENT.14
2.3 B-LINEAGE CELLS IN EXPERIMENTALLY INDUCED IMMUNE
RESPONSE.17
2.3.1
IMMUNIZATION.
17
2.3.2 FACTORS FAVORING B-CELL SURVIVAL
IN
VITRO.17
2.4 ONCOGENE DRIVEN B-CELL
DEVELOPMENT.
19
2.4.1
MYC.
19
2.4.2
BCL-XL.21
2.4.3 DEVELOPMENT OF *DOUBLE-HIT* B-LYMPHOID
MALIGNANCIES.23
2.4.4 TRANSFORMING GENES FROM
CDNA-LIBRARIES.
24
2.5 AIMS OF THE RESEARCH
PROJECT.
25
3 MATERIALS AND
METHODS.26
3.1
MATERIALS.26
3.1.1
MACHINES.
26
3.1.2
DISPOSABLES.
27
3.1.3
SOFTWARE.
28
3.1.4 K
ITS.29
3.1.5
CHEMICALS.
29
3.1.6 BUFFERS &
SOLUTIONS.30
3.1.7 MOLECULAR- AND CELL-BIOLOGY
REAGENTS.
32
3.1.8
PRIMER.33
3.1.9
ENZYMES.
34
3.1.10
ANTIBODIES.
34
3.1.11
MICROBIOLOGY.
36
3.1.12 TISSUE
CULTURE.
36
3.1.13 PLASMIDS AND
VECTORS.
37
3.1.14 CELL
LINES.
38
3.1.15 CYTOKINE
PRODUCERS.39
3.1.16 MOUSE
STRAINS.40
3.2
METHODS.40
3.2.1 MICROBIOLOGICAL
METHODS.40
3.2.2 MOLECULAR
METHODS.41
3.2.3 IMMUNOLOGICAL
METHODS.
48
3.2.4 CELL
CULTURE.
51
3.2.5. ANIMAL W
ORK.58
4 RESULTS PROJECT
1.
61
4.1 EFFECTS OF C-MYC AND BCL-XL OVEREXPRESSION ON PREBI-CELLS IN
VITRO.61
4.1.1 EFFECTS OF C-MYC AND/OR BCL-XL OVEREXPRESSION ON ENTRY INTO CELL
CYCLE AND APOPTOSIS OF
DIFFERENTIATING
PREBI-CELLS.
61
4.1.2 JOINT EXPRESSION OF C-MYC AND BCL-XL PROVIDES PREB-CELLS LONGEVITY
IN VITRO
.
62
4.1.3 CHANGES OF SURFACE MARKER EXPRESSION OF C-MYC/BCL-XL PREBI-CELLS
WITHIN 3 WEEKS OF IN
VITRO
DIFFERENTIATION.64
4.1.4 GENE EXPRESSION OF C-MYC/BCL-XL IMMATURE B-CELLS DIFFERENTIATED
DAYS 18 IN VITRO
.
65
4.1.5 POLYCLONAL STIMULATION OF IN VITRO DIFFERENTIATED IMMATURE
B-CELLS.66
4.1.6 CPG-STIMULATION OF IN VITRO DIFFERENTIATING, C-MYC/BCL-XL
OVER-EXPRESSING B-LINEAGE CELLS.
4.1.7 FIRST BRIEF SUMMARY DESCRIBING THE B-CELL TYPES GENERATED IN
VITRO.70
4.1.8 REARRANGEMENT STATUS OF IN VITRO DIFFERENTIATING, CPG-STIMULATED
C-MYC/BCL-XL CELLS
.
70
4.1.9 CPG STIMULATION OF C-MYC/BCL-XL B-LINEAGE-CELLS INCREASES
SECONDARY IGL REARRANGEMENT IN
VITRO.72
4.1.10 C-MYC/BCL-XL IMMATURE B-CELLS THAT DIFFERENTIATED FOR 32 DAYS
SECRETE IGM AND EXPRESS
CD40, CD80 AND MUCH MOLECULES UPON STIMULATION IN VITRO
.
73
4.1.11 SECOND BRIEF SUMMARY DESCRIBING THE B-CELL TYPES GENERATED IN
VITRO
.
75
4.2 C-MYC/BCL-XL SINGLE CELL
CULTURES.
76
4.2.1 CLONING OF C-MYC/BCL-XL OVEREXPRESSING CELLS BY SINGLE CELL
SORTING INTO CULTURES CONTAINING
RAT THYMUS *FILLER*
CELLS.
76
4.2.2 C-MYC/BCL-XL CLONES DO NOT CHANGE THEIR PHENOTYPE AND CAN BE
STIMULATED TO SECRETE IGM
IN
VITRO.77
4.2.3 SUMMARY OF THE IN VITRO
EXPERIMENTS.
79
4.3 TRANSPLANTATION OF C-MYC/BCL-XL
PREBI-CELLS.
80
4.3.1
4.3.2
4.3.3
4.3.4
4.3.5
4.3.6
4.3.7
C-MYC/BCL-XL PREBI-CELL TRANSPLANTATION INTO IMMUNODEFICIENT RAGL"" M
ICE
.
80
EX VIVO/IN VITRO CULTURE OF IN VIVO EXPANDED GFP+ C-MYC/BCL-XL B-LINEAGE
CELLS FROM RAG""
RECIPIENTS.
82
RE-TRANSPLANTATION OF BONE MARROW-DERIVED, IN VIVO EXPANDING GFP+
C-MYC/BCL-XL B-
LINEAGE CELLS INTO SECONDARY
HOSTS.84
NP-CGG-IMMUNIZATION OF C-MYC/BCL-XL PREBI-CELL-TRANSPLANTED RECIPIENTS
.
86
IN VITRO CULTURES OF IN VIVO PROLIFERATING GFP+ C-MYC/BCL-XL B-LINEAGE
CELLS FROM NP-CGG-
IMMUNIZED
MICE.89
NP-OVA-IMMUNIZATION OF C-MYC/BCL-XL PREBI-CELL-TRANSPLANTED RAGL "
'RECIPIENTS
.
90
EX VIVO/IN VITRO CULTURES OF GFP+ C-MYC/BCL-XL B-LINEAGE CELLS FROM
NP-OVA-IMMUNIZED
M
ICE.94
4.3.8 CO-TRANSPLANTATION OF CD4+ HELPER T-CELLS INTO RAGL"" MICE
TRANSPLANTED WITH C-MYC/BCL-XL
PREBI-CELLS.
95
4.3.9 IN VITRO CULTURES OF PROLIFERATING C-MYC/BCL-XL-OVEREXPRESSING
GFP+ CELLS FROM CO
TRANSPLANTED MICE IMMUNIZED WITH
NP-OVA.99
4.3.10/ VIVO
SUMMARY.100
5 RESULTS PROJECT 2
.101
5.1 GENERATION OF AN EXPRESSION
CDNA-LIBRARY.
101
5.1.1 CLONING OF
CDNA-LIBRARIES.101
5.1.2 QUALIFICATION OF THE
CDNA-LIBRARY.
102
5.2 C-MYC-TRANSGENIC PREB-CELLS AS TARGETS FOR TRANSDUCTIONS WITH
CDNA-LIBRARIES
.
104
5.3 PROOF OF PRINCIPLE EXPERIMENTS - SERIALLY DILUTED PIM-1 EXPRESSION
AS PREDICTOR FOR COOPERATIVE
ACTIVITIES OF C-MYC AND CDNA-LIBRARY GENES FOR FULL TRANSFORMATION
.
106
5.4 TRANSFORMATION OF C-MYC-TRANSGENIC PREBI-CELLS WITH CDNA EXPRESSION
LIBRARY-DERIVED GENES IN
VITRO.107
5.5 EXOSCL AS A TRANSFORMING GENE COOPERATING WITH C-MYC IN B-CELL
TRANSFORMATION
.
108
5.5.1 IDENTIFICATION OF A SHORT-FORM OF THE EXOSCL GENE COOPERATING WITH
C-MYC IN B-CELL
TRANSFORMATION.108
5.5.2 C-MYC/EXOSCLSF IMMATURE-LIKE B-CELLS EXPAND AND DIFFERENTIATE TO
MATURE B1-CELLS IN VIVO
.109
5.5.3 TRANSFORMING ACTIVITY OF THE FULL-LENGTH EXOSCL TRANSCRIPT
.
.
111
5.6 POOLS OF PREBI-CELLS OVEREXPRESSING C-MYC TOGETHER WITH DIFFERENT
CDNA-LIBRARY GENES EXPAND IN
VIVO TO DIFFERENT B-LINEAGE
STAGES.113
5.7 IDENTIFICATION OF THE CDNA-LIBRARY GENES THAT MIGHT COOPERATE WITH
C-MYC IN B-LINEAGE CELL
TRANSFORMATION IN
VIVO.115
6
DISCUSSION.
117
6.1 DISCUSSION PROJECT 1: THE IMPACT OF C-MYC AND BCL-XL IN B-CELL
TRANSFORMATION
.
117
6.2 DISCUSSION PROJECT 2: DETECTION OF TRANSFORMING GENES FROM
DOXYCYCLINE-INDUCIBLE CDNA
EXPRESSION LIBRARIES IN C-MYC-TRANSGENIC PREB-CELLS AND THEIR PROGENY
.
122
7
REFERENCES.125
8 FIGURE L
IST.
140
8.1 INTRODUCTION
FIGURES.140
8.2 MATERIAL AND METHODS
FIGURES.
140
8.3 RESULT
FIGURES.
140
8.4 SUMMARY
FIGURES.
142
9 LIST OF
ABBREVIATIONS.143
10 COPYRIGHT AND
PERMISSION.
143 |
| any_adam_object | 1 |
| author | Wolf, Ingrid |
| author_GND | (DE-588)1128184036 |
| author_facet | Wolf, Ingrid |
| author_role | aut |
| author_sort | Wolf, Ingrid |
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| building | Verbundindex |
| bvnumber | BV043874998 |
| ctrlnum | (OCoLC)978272960 (DE-599)BVBBV043874998 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV043874998 |
| illustrated | Illustrated |
| indexdate | 2025-02-11T19:00:35Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029284755 |
| oclc_num | 978272960 |
| open_access_boolean | |
| owner | DE-83 |
| owner_facet | DE-83 |
| physical | 145 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| record_format | marc |
| spelling | Wolf, Ingrid Verfasser (DE-588)1128184036 aut Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells vorgelegt von Dipl.-Ing. Ingrid Wolf, geb. in Shanatas, Kasachstan Berlin 2016 145 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Technische Universität Berlin 2016 (DE-588)4113937-9 Hochschulschrift gnd-content http://services.ub.tu-berlin.de/abstracts/diss/wolf_ingrid_abstract.pdf Abstract http://services.ub.tu-berlin.de/abstracts/diss/wolf_ingrid_inhalt.pdf Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029284755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wolf, Ingrid Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells |
| subject_GND | (DE-588)4113937-9 |
| title | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells |
| title_auth | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells |
| title_exact_search | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells |
| title_full | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells vorgelegt von Dipl.-Ing. Ingrid Wolf, geb. in Shanatas, Kasachstan |
| title_fullStr | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells vorgelegt von Dipl.-Ing. Ingrid Wolf, geb. in Shanatas, Kasachstan |
| title_full_unstemmed | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells vorgelegt von Dipl.-Ing. Ingrid Wolf, geb. in Shanatas, Kasachstan |
| title_short | Retroviral overexpression of genes that induce transformation at different stages of B-cell development from preBI-cells to plasma cells |
| title_sort | retroviral overexpression of genes that induce transformation at different stages of b cell development from prebi cells to plasma cells |
| topic_facet | Hochschulschrift |
| url | http://services.ub.tu-berlin.de/abstracts/diss/wolf_ingrid_abstract.pdf http://services.ub.tu-berlin.de/abstracts/diss/wolf_ingrid_inhalt.pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029284755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wolfingrid retroviraloverexpressionofgenesthatinducetransformationatdifferentstagesofbcelldevelopmentfromprebicellstoplasmacells |
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