Lentiviral vectors and exosomes as gene and protein delivery tools:
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York
Humana Press
[2016]
|
| Series: | Methods in molecular biology
1448 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xi, 276 Seiten Illustrationen |
| ISBN: | 9781493937516 |
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MARC
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Record in the Search Index
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| adam_text | Titel: Lentiviral vectors and exosomes as gene and protein delivery tools
Autor: Federico, Maurizio
Jahr: 2016
Contents
Preface.......................................................... v
Contributors...................................................... ix
Part I Recent Improvements in LV Construction, Production,
and Transduction
1 Construction of Modular Lentiviral Vectors for Effective
Gene Expression and Knockdown.................................. 3
Angeline de Bruyns, Ben Gelling, and David Dankort
2 Development of Inducible Molecular Switches Based on All-in-One
Lentiviral Vectors Equipped with Drug Controlled FLP Recombinase....... 23
Tobias Maetzig and Axel Schambach
3 Production of Retrovirus-Based Vectors in Mildly Acidic pH Conditions .... 41
Nathalie Holic and David Fenard
4 Optimized Lentiviral Transduction Protocols by Use of a Poloxamer
Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G........... 49
NataSa Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt,
and Christian Thirion
Part II New LV Targets and Applications
5 Transduction of Murine Hematopoietic Stem Cells
with Tetracycline-regulated Lentiviral Vectors......................... 65
Maike Stahlhut, Axel Schambach, and Olga S. Kustikova
6 Introduction of shRNAs, miRNAs, or AntagomiRs into Primary
Human Liver Cells Through Lentiviral Vectors........................ 77
Jessica K. Rieger and Maria Thomas
7 Production and Concentration of Lentivirus for Transduction
of Primary Human T Cells....................................... 85
Alan Kennedy and Adam P. Cribbs
8 Generating Transgenic Mice by Lentiviral Transduction
of Spermatozoa Followed by In Vitro Fertilization
and Embryo Transfer........................................... 95
Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead,
and Robert Winston
9 The LAM-PCR Method to Sequence LV Integration Sites............... 107
Wei Wang, Cynthia C. Bartholomae, Richard Gabriel,
Annette Deichmann, and Manfred Schmidt
vii
viii
Contents
10 Conditional RNAi Using the Lentiviral GLTR System................... 121
Elisabeth Pfeijfenberger, Reinhard Sigl, and Stephan Geley
11 Lentiviral Vectors for the Engineering of Implantable
Cells Secreting Recombinant Antibodies............................. 139
Aur6lien Lathuiliire and Bernard L. Schneider
Part III Integrase-Mutant LVs
12 Transient Expression of Green Fluorescent Protein
in Integrase-Defective Lentiviral Vector-Transduced. 293T Cell Line........ 159
Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan,
Farzin Farzaneh, and M. K. Azaham A. Hamid
13 Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors
in a Rat Model of Parkinson s Disease............................... 175
Njjoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. YAnez-Munoz
14 Development of Lentiviral Vectors for Targeted Integration
and Protein Delivery............................................ 185
Diana Schenkwein and Seppo Yld-Herttuala
Part IV Production, Detection, and Engineering of Exosomes
15 Biogenesis and Functions of Exosomes and Extracellular Vesicles........... 201
Florian Dreyer and Andreas Baur
16 Generation, Quantification, and Tracing of Metabolically
Labeled Fluorescent Exosomes.................................... 217
Carolina Coscia, Isabella Parolini, Massimo Sanchez,
Mauro Biffoni, Zaira Boussadia, Cristiana Zanetti,
Maria Luisa Fiani, and Massimo Sargiacomo
17 Cardiac Myocyte Exosome Isolation................................ 237
ZulfiqarA. Malik, Tingting T. Liu, and Anne A. Knowlton
18 Incorporation of Heterologous Proteins in Engineered Exosomes.......... 249
Francesco Manfredi, Paola Di Bonito, Claudia Arenaccio,
Simona Anticoli, and Maurizio Federico
19 Exosome-Mediated Targeted Delivery of miRNAs...................... 261
Shin-ichiro Ohno and Masahiko Kuroda
Index........................................................... 271
|
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| id | DE-604.BV043757398 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:34:17Z |
| institution | BVB |
| isbn | 9781493937516 |
| language | English |
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| physical | xi, 276 Seiten Illustrationen |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy New York Humana Press [2016] 2016 xi, 276 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1448 Springer protocols LentivirusesvLaboratory manuals Lentivirus Laboratory Manuals Federico, Maurizio edt Erscheint auch als Online-Ausgabe 978-1-4939-3753-0 Methods in molecular biology 1448 (DE-604)BV035362695 1448 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029168831&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lentiviral vectors and exosomes as gene and protein delivery tools Methods in molecular biology LentivirusesvLaboratory manuals Lentivirus |
| title | Lentiviral vectors and exosomes as gene and protein delivery tools |
| title_auth | Lentiviral vectors and exosomes as gene and protein delivery tools |
| title_exact_search | Lentiviral vectors and exosomes as gene and protein delivery tools |
| title_full | Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy |
| title_fullStr | Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy |
| title_full_unstemmed | Lentiviral vectors and exosomes as gene and protein delivery tools edited by Maurizio Federico, National AIDS Center, Istituto Superiore di Sanità, Rome, Italy |
| title_short | Lentiviral vectors and exosomes as gene and protein delivery tools |
| title_sort | lentiviral vectors and exosomes as gene and protein delivery tools |
| topic | LentivirusesvLaboratory manuals Lentivirus |
| topic_facet | LentivirusesvLaboratory manuals Lentivirus |
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