IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice:
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| Format: | Thesis Microfilm Book |
| Language: | English |
| Published: |
Köln
[2015]
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 2 Mikrofiches (101 Blätter) Illustrationen 21 cm |
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| adam_text | Titel: IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in selec
Autor: Jakobshagen, Kristin
Jahr: 2015
Table of contents
Abbreviations
1 Introduction
1.1 lnlerleukin-10 1
1.2 Lymphocytic choriomeningitis virus (LCMV) 2
1.3 Basics of LCMV-specific immune response 3
1.4 Impact of IL-10 on the chronic LCMV infection 4
1.5 Impact of cytokine milieus on T cells 5
1.6 Aim of this work 5
2 Materials and Methods
2.1 Material 6
2.1.1 Mice 6
2.1.2 Virus 7
2.1.3 Cell lines 7
2.1.4 Peptides 7
2.1.5 Cell culture media, chemicals, buffers and solutions 8
2.1.6 Antibodies 10
2.1.7 Kits 12
2.1.8 Technical equipment 13
2.1.9 Consumables 14
2.1.10 Software 14
2.2 Methods 15
2.2.1 Cell culture 15
2.2.2 Infection of mice with LCMV 15
2.2.3 Virus titration 15
2.2.4 Preparation of CD4+ and CD8+ T cells from spleens 16
2.2.4.1 Splenic single cell suspensions 16
2.2.4.2 Immunomagnetic enrichment of T cells from spleens 17
2.2.5 Assay for virus-specific cytotoxicity of CD8+ T cells 17
2.2.6 Flow cytometry 18
2.2.6.1 Staining of surface molecules 18
2.2.6.2 Intracellular staining of cytotoxic effector molecules 19
2.2.7 Stimulation of LCMV-specific T cells 19
2.2.7.1 Preparation of target cells for antigen-specific T cell stimulation 20
2.2.7.2 Antigen-specific stimulation of CDS* T cells or CD4+ T cells 20
2.2.7.3 PMA and ionomycin induced cytokine secretion 21
2.2.8 Quantification of cytokines 21
2.2.8.1 Quantification of cytokines in supernatants 21
2.2.8.2 Quantification of cytokines in serum samples 21
2.2.9 Quantification of GzmB 22
2.2.10 Induction of LCMV-specific immunopathological syndromes 22
2.2.10.1 Delayed type hypersensitivity (DTH) reaction 22
2.2.10.2 LCMV-induced hepatitis 23
2.2.10.3 Clinical course and histopathology of lymphocytic choriomeninigits 23
2.2.11 Statistical analysis 24
3 Results
3.1 Deficiency in IL-10 leads to accelerated LCMV elimination
during acute infection of mice 26
3.2 Deficiency in IL-10 leads to enhanced CDS* T cell responses
during acute infection of mice with LCMV 28
3.3 Influence of IL-10 on cytotoxic effector pathways 34
3.4 Identification of the IL-10 producing cell type crucial for
down-modulating LCMV-specific immune responses 45
3.4.1 T cell-derived IL-10 is involved in down-modulating
LCMV-specific immune responses in C57BL/6 and BALB/c mice 46
3.4.2 DC-derived IL-10 contributes to down-modulation of the
LCMV-specific immune responses in C57BL/6 and BALB/c mice 48
3.4.3 Search for a contribution of IL-10 from other cell types 50
3.5 CD4* T cells support the expansion and cytotoxic activity of
LCMV-specific CDS* T cells in IL-10 mice 53
3.6 IL-10 deficiency increases cytokine secretion by T cells
during acute LCMV infection 59
3.7 Effects of IL-10 deficiency on LCMV-specific
immunopathological responses 64
3.7.1 IL-10 limits the LCMV-induced DTH reaction 64
3.7.2 LCMV-induced acute hepatitis 66
3.7.3 Influence of IL-10 on LCMV-induced choriomeningitis 70
4 Discussion 79
5 References 86
6 Summary 99
6 Zusammenfassung 100
7 Erklarung 101
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| spelling | Jakobshagen, Kristin Verfasser (DE-588)1080676023 aut IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice vorgelegt von Kristin Jakobshagen geb. Nachtwey Köln [2015] 2 Mikrofiches (101 Blätter) Illustrationen 21 cm txt rdacontent h rdamedia he rdacarrier 24x Dissertation Universität zu Köln 2015 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028993619&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Jakobshagen, Kristin IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice |
| subject_GND | (DE-588)4113937-9 |
| title | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice |
| title_auth | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice |
| title_exact_search | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice |
| title_full | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice vorgelegt von Kristin Jakobshagen geb. Nachtwey |
| title_fullStr | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice vorgelegt von Kristin Jakobshagen geb. Nachtwey |
| title_full_unstemmed | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice vorgelegt von Kristin Jakobshagen geb. Nachtwey |
| title_short | IL-10 alleviates the systemic antiviral T cell-mediated immune response and immunopathology in select organs of acutely LCMV-infected mice |
| title_sort | il 10 alleviates the systemic antiviral t cell mediated immune response and immunopathology in select organs of acutely lcmv infected mice |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028993619&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT jakobshagenkristin il10alleviatesthesystemicantiviraltcellmediatedimmuneresponseandimmunopathologyinselectorgansofacutelylcmvinfectedmice |