Nutrients regulate flowering time:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Potsdam
2015
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 190 Seiten Illustrationen, Diagramme |
Internformat
MARC
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
SUMMARY 4
ZUSAMMENFASSUNG 6
ACKNOWLEDGEMENTS AND CONTRIBUTIONS 9
LISTS OF ABBREVIATIONS 11
TABLE OF CONTENTS 17
LIST OF FIGURES 21
LIST OF SUPPLEMENTARY FIGURES 23
LIST OF TABLES 24
LIST OF SUPPLEMENTARY TABLES 25
1 INTRODUCTION 26
1.1 NITROGEN 26
1.1.1 NITRATE UPTAKE 26
1.1.2 AMMONIUM UPTAKE 28
1.1.3 NITRATE TRANSPORTERS 28
1.1.4 NITRATE ASSIMILATION 29
1.1.5 NITRATE SENSORS AND SIGNALLING 31
1.1.6 NITRATE RESPONSIVE ELEMENTS (NRE) 33
1.1.7 NITRATE IMPORTANCE IN AGRICULTURE 34
1.1.7.1 NITRATE IMPORTANCE FOR PLANT GROWTH AND DEVELOPMENT 34
1.2 FLOWERING TIME REGULATION 37
1.2.1 PHOTOPERIOD PATHWAY 40
1.2.2 TEMPERATURE PATHWAY 42
1.2.3 GIBBERELLIC ACID (GA) PATHWAY 43
1.2.4 AUTONOMOUS PATHWAY 44
1.2.5 AGE PATHWAY 44
1.2.6 T6P/7PS1 PATHWAY 45
1.3 GROWTH SYSTEMS AND CONTROLLED N SUPPLY 46
1.4 AIMS AND OBJECTIVES 48
2 MATERIAL AND METHODS 50
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HTTP://D-NB.INFO/1082518468
2.1 PLANT MATERIALS 50
2.2 PLANT GROWTH CONDITIONS 51
2.2.1 N-LIMITED SOIL SYSTEM 51
2.3 CHEMICALS 51
2.4 PHENOTYPE ANALYSIS 52
2.4.1 PLANT SIZE, RGR MEASUREMENT AND FRESH WEIGHT 52
2.4.2 FLOWERING TIME 52
2.4.3 LEAF INITIATION RATE 52
2.4.4 MEASUREMENT OF JUVENILE LEAVES 53
2.4.5 RNA IN SITU HYBRIDISATION AND HISTOLOGICAL STAINING 53
2.4.5.1 CLONING 53
2.4.5.2 OLIGONUCLEOTIDES AND PCR 53
2.4.5.3 A-TAILING AND AT CLONING 54
2.4.5.4 TRANSFORMATION OF E.COLI (DH5A) 54
2.4.5.5 PLASMID PREPARATION 55
2.4.5.6 PROBE SYNTHESIS 55
2.4.5.7 FIXATION, EMBEDDING AND SECTIONING 56
2.4.5.8 RNA IN SITU HYBRIDISATION 57
2.4.5.9 TOLUIDINE BLUE STAINING 58
2.5 RNA EXTRACTION, CDNA SYNTHESIS AND QRT-PCR ANALYSIS 59
2.5.1 RNA EXTRACTION 59
2.5.2 CDNA SYNTHESIS 60
2.5.3 QRT-PCR ANALYSIS 60
2.5.3.1 OLIGONUCLEOTIDES 60
2.5.3.2 TRANSCRIPT PROFILING 61
2.6 EXTRACTION AND MEASUREMENT OF METABOLITES 62
2.6.1 DETERMINATION OF CHLOROPHYLLS, SUGARS, STARCH, TOTAL AA AND
PROTEIN 62
2.6.2 DETERMINATION OF SINGLE STANDARD AMINO ACIDS 62
2.6.3 DETERMINATION OF T6P 62
2.7 NRES ANALYSIS 63
3 COMPARISON OF WILD-TYPE PLANTS AND FLOWERING MUTANTS GROWING IN LN AND
NN IN LONG DAY CONDITIONS 64
3.1 BACKGROUND 64
3.2 AIMS AND EXPERIMENTAL DESIGN 65
3.3 MORPHOLOGICAL PHENOTYPE OF COL-0 PLANTS 65
3.4 GENETICS: DETERMINING FLOWERING TIME OF MUTANT LINES 67
18
3.5 CHAPTER SUMMARY AND DISCUSSION 69
4 SOIL BASED SHORT DAY TO LONG DAY SHIFT EXPERIMENT 71
4.1 BACKGROUND 71
4.2 AIMS AND EXPERIMENTAL DESIGN 72
4.3 MORPHOLOGICAL PHENOTYPE OF COL-0 PLANTS 73
4.4 PHYSIOLOGICAL CHARACTERISATION OF COL-0 PLANTS: METABOLITES 76
4.5 EFFECT OF NITROGEN SUPPLY IN THE SOIL ON EXPRESSION OF NIA1 AND NIA2
AT THE SAM 78
4.6 IMPACT OF THE NITROGEN SUPPLY IN THE SOIL ON EXPRESSION OF GENES
INVOLVED IN THE REGULATION OF FLOWERING
TIME 80
4.6.1 MOLECULAR EVIDENCE FOR A DELAY OF FLORAL TRANSITION IN LN GROWN
PLANTS 80
4.6.2 INVESTIGATION OF THE POTENTIAL REASON OF THE DELAY IN FLOWERING
TIME IN LN PLANTS 81
4.6.2.1 ANALYSIS OF FLORAL REPRESSOR TFL1 81
4.6.2.2 ANALYSIS OF THE COMPONENTS OF THE PHOTOPERIOD PATHWAY 81
4.6.2.3 ANALYSIS OF SOC1 REGULATORS 86
4.7 CHAPTER SUMMARY AND DISCUSSION 88
5 COMPARISON OF WILD-TYPE PLANTS AND FLOWERING MUTANTS GROWN IN LN AND
NN IN SHORT DAY CONDITIONS 91
5.1 BACKGROUND 91
5.2 AIMS AND EXPERIMENTAL DESIGN 92
5.3 MORPHOLOGICAL PHENOTYPE OF COL-0 PLANTS 93
5.4 PHYSIOLOGICAL CHARACTERISATION OF COL-0 PLANTS: METABOLITES 97
5.5 IMPACT OF THE NITROGEN SUPPLY IN THE SOIL ON EXPRESSION OF N MARKER
GENES AND P STARVATION RESPONSIVE
GENES 100
5.5.1 EFFECT OF NITROGEN SUPPLY IN THE SOIL ON EXPRESSION OF NIA2 AT THE
SAM 102
5.6 EFFECT OF NITROGEN SUPPLY ON GENES INVOLVED IN THE FLOWERING TIME
103
5.6.1 IMPACT OF N SUPPLY ON GENES INVOLVED IN THE FLOWERING TIME
ANALYSED BY QRT-PCR IN WHOLE ROSETTE
SAMPLES 103
5.6.2 IMPACT OF N SUPPLY ON GENES INVOLVED IN THE FLOWERING TIME
ANALYSED BY QRT-PCR AND RNA IN SITU
HYBRIDISATION AT THE SAM 105
5.6.3 MOLECULAR EVIDENCE FOR A DELAY OF FLORAL TRANSITION IN LN GROWN
PLANTS 106
5.6.4 ANALYSIS OF N IMPACT OF KEY PLAYERS OF THE FLOWERING NETWORK AT
THE SAM 107
5.6.4.1 ANALYSIS OF FUL AT THE SAM 107
5.6.4.2 ANALYSIS OF SOC1 AND SOC1 REGULATORS AT THE SAM 109
5.6.4.3 ANALYSIS OF SPL5 AT THE SAM 110
5.7 DETERMINING FLOWERING TIME OF MUTANT LINES 112
5.8 N-DEPENDENT MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERISATION OF THE
35S::AMIRTPSL 115
5.9 CHAPTER SUMMARY AND DISCUSSION 116
19
6 FINAL DISCUSSION 119
6.1 FLOWERING TIME IS DELAYED IN COL-0 PLANTS GROWN IN LOW N 121
6.2 LOW N SUPPLY AFFECTS BIOMASS, ROSETTE AREA AND RGR OF COL-0 PLANTS
123
6.3 LOW N CONTENT IN THE SOIL INFLUENCES N AND C METABOLISM AND
EXPRESSION OF N MARKER GENES 124
6.4 N-DEPENDENT SIGNALLING - SIGNAL PERCEPTION SITES AT THE SAM 126
6.4.1 THE PHOTOPERIOD PATHWAY IS NOT INVOLVED IN N-DEPENDENT SIGNALLING
127
6.4.2 NITRATE MIGHT ACT ON SOC1 EXPRESSION AT THE SAM 129
6.4.3 NITRATE MIGHT ALSO DIRECTLY ACT ON SPL3 AND SPL5 EXPRESSION AT THE
SAM 131
6.4.4 NITRATE MIGHT DIRECTLY ACT ON FUL EXPRESSION AT THE SAM 133
6.4.5 SVP/FLC REPRESSOR COMPLEX ACTIVE IN LN GROWN PLANTS 134
6.4.6 HYPOTHETICAL AND SIMPLIFIED MODEL OF N-SIGNALLING IN THE
REGULATION OF FLOWERING TIME 135
6.5 THE ONSET OF FLOWERING IN LN GROWN PLANTS IN THE SD PHOTOPERIOD 136
6.6 EARLY FLOWERING PHENOTYPE OF 35S::MIR156 IN LN CONDITIONS IN SD
PHOTOPERIOD 138
7 OUTLOOK 140
7.1 CONFIRM IF NITRATE ACTS DIRECTLY ON SOC1, SPL3 AND SPL5 EXPRESSION
VIA THE NRES 140
7.2 FURTHER ANALYSIS OF SVP/FLC COMPLEX 140
7.3 INTERACTION AND EPISTATIC RELATION ANALYSIS BETWEEN FLC, SVP AND
SOC1 141
7.4 QRT-PCR ANALYSIS USING APEX SAMPLES FROM SD TO LD SHIFT EXPERIMENT
142
7.5 INVESTIGATION OF THE POTENTIAL REASON OF EARLY FLOWERING PHENOTYPE
OF 35S :MIR156 IN LN CONDITIONS IN SD
PHOTOPERIOD 142
7.6 RNA IN SITU HYBRIDISATION USING CNC AND GNL AS PROBES 143
8 SUPPLEMENTAL MATERIAL 144
8.1 SUPPLEMENTAL FIGURES 144
8.2 SUPPLEMENTAL TABLES 155
9 LITERATURE 169
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| spelling | Olas, Justyna Jadwiga Verfasser (DE-588)1089080654 aut Nutrients regulate flowering time von Justyna Jadwiga Olas Potsdam 2015 190 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Blühinduktion (DE-588)4146050-9 gnd rswk-swf Stickstoffdüngung (DE-588)4057513-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Blühinduktion (DE-588)4146050-9 s Stickstoffdüngung (DE-588)4057513-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028889788&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Olas, Justyna Jadwiga Nutrients regulate flowering time Blühinduktion (DE-588)4146050-9 gnd Stickstoffdüngung (DE-588)4057513-5 gnd |
| subject_GND | (DE-588)4146050-9 (DE-588)4057513-5 (DE-588)4113937-9 |
| title | Nutrients regulate flowering time |
| title_auth | Nutrients regulate flowering time |
| title_exact_search | Nutrients regulate flowering time |
| title_full | Nutrients regulate flowering time von Justyna Jadwiga Olas |
| title_fullStr | Nutrients regulate flowering time von Justyna Jadwiga Olas |
| title_full_unstemmed | Nutrients regulate flowering time von Justyna Jadwiga Olas |
| title_short | Nutrients regulate flowering time |
| title_sort | nutrients regulate flowering time |
| topic | Blühinduktion (DE-588)4146050-9 gnd Stickstoffdüngung (DE-588)4057513-5 gnd |
| topic_facet | Blühinduktion Stickstoffdüngung Hochschulschrift |
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