Gnotobiotic mouse technology: an illustrated guide
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| Main Authors: | , , |
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| Format: | Book |
| Language: | English |
| Published: |
Boca Raton ; London ; New York
CRC Press
© 2016
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | xvi, 237 Seiten Illustrationen |
| ISBN: | 9781498736329 1498736327 |
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MARC
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Record in the Search Index
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| adam_text | Contents
Preface and Acknowledgments................................xiii
About the Authors..........................................xv
1 A Brief History of Germ-Free Life.................... 1
References....................................... 4
2 Overview of Gnotobiotic Technology.................... 5
What Are Germ-Free Animals?.............................5
How Do We Get Germ-Free Mice?...........................6
Germ-Free Housing and Barrier Types.....................7
References......................................... 10
3 Equipment and Terminology............................ 11
4 Personal Protective Equipment (PPE).................. 19
Introduction....................................... 19
Types of PPE..........................................20
Applying Sterile PPE............................... 21
Supplies.........................-...................21
Procedure ........................................ 21
5 Sterilants and Sterilization..................... 27
Introduction..........................................27
Sterilization Methods.......................... ....27
vii
viii Contents
Cold Sterilization...........................................28
Supplies.............................-..................28
Procedure ............................................. 30
Wrapping Materials for Autoclaving...........................32
Supplies.................................................32
Procedure............................*..................32
Gas Sterilization (Ethylene Oxide)...........................34
Supplies.................................................34
Procedure......................*........................34
6 Isolator Setup..............................................39
Introduction...........*....................................39
Part 1: Small (5.5 x 3 feet) Isolator Assembly...............41
Preparation of Isolator Components .................... 41
Supplies.............................................42
Isolator Components................................ 43
Preparing Hand-Wrapped Filters......................... 43
Supplies......................................... 43
Installing the Isolator Components.......................50
Inflating and Sterilizing the Isolator...................59
Decontaminate the Inside of the Isolator.................64
Overnight Leak Test......................................66
Start the Isolator..................................... 66
Part 2: Large Isolator Assembly..............................69
Introduction......................................... 69
Supplies........................................... 69
Preparation...........................*...................71
Sterilize the Filters................................71
Sterilize the Air Supply Piping System...............76
Prepare the Supplies............................... 76
Isolator Assembly.................................... 77
Install the Gloves............................... ...77
Assemble the Airflow Piping.................. ....84
Install the Intake and Exhaust Filters...............88
Stock and Inflate the Isolator.................... ,92
Clean the Inside of the Isolator.....................96
Start the Isolator.................. ...............99
Prefilters......................................... 99
Contents ix
7 Port Entry and Exit...................................... 101
Introduction............................,..............101
Supplies............................................... 101
Preparing, Opening, and Closing the Port.................102
Proper Placement of Materials............................107
8 Sterilizing Food and Supplies............................109
Introduction........................................... 109
Sterility Indicators................................... 109
Assembling Supply Cylinders: Installing HEPA Filtration
Media....................................................112
Supplies............................................... 112
Prepare the Cylinder................................. 113
Fill the Cylinder.................................... 114
Water Sterilization.................................... 122
Supplies..............................................125
Procedure....................................... 126
9 Supply Cylinder Entry................................ 129
Introduction.............................................129
Supplies............................................. 130
Procedure.......................................... 130
10 Isolator Maintenance.....................................139
Introduction.............................................139
Part 1: Cleaning and Maintenance....................... 139
Part 2: Replacing Gloves on an Active Isolator...........142
Procedure......................................... 143
Part 3: Repairing Holes in Isolators.....................147
Part 4: Working in the Isolator...................... 149
Sample and Tube Labeling Inside the Isolator..........149
Weighing Mice.........................................149
Options for Weighing Mice....................... 149
Use of the Pesola Scale for Weighing Mice Aseptically....................................... 150
11 Aseptic Mouse Transfer ............................ 155
Introduction..................................... 155
x Contents
Part 1: Internal Transfer between isolators.............155
Introduction.........................................155
Supplies.............................................156
Procedure............................................156
Part 2: External Transfer of Mice.......................157
Procedure............................................157
12 Working with Germ-Free or Gnotobiotic Mice
in the Class II Biosafety Cabinet.......................161
Introduction..................................-.........161
Supplies................................................162
Procedure........................................... 162
Experimental Manipulations in the Biosafety Cabinet.....164
Introduction.....................................*---164
Procedures...........................................166
13 Shipping Mice...........................................171
Introduction.......................................... 171
Procedure........................................... 172
14 Rederivation..................................... 181
Introduction.................................... 181
Derivation by Cesarean Section........................ 182
Derivation by Embryo Transfer..................... 184
References........................................ 188
15 Microbiological Testing............................. 189
Introduction.................................. 189
Part 1: Screening for Bacterial Contamination......... 191
Supplies................................. 191
Sample Collection................................... 191
Bacterial Culture and Slide Preparation............. 191
Gram Stain Procedure............................. 195
Quantitative PGR............................... 196
Part 2: Examination of Gram-Stained Slides for the
Presence of Bacteria....................................197
References.......................................... 2Q3
Contents xi
16 Genetic Testing...................................... 205
References...........................................208
17 Record Keeping..........................,.............209
Introduction........................................ 209
Colony Management Documents..........................210
Population Census..................................210
Breeding Records..................................... 210
Genetic Monitoring............................. 213
Isolator Activity Logs......................... 213
Project-Planning Documents........................ 213
Germ Free Mouse User Request Form................ 213
Mouse Project Requests.......................... 215
Experimental Space Availability.................. 218
Other Documents................................. 218
Bacterial Screening Log.......................... 218
Health Monitoring.............................. 218
Paper Documents.............................. 220
18 Facility Setup and Management........................ 223
Introduction......................................... 223
Establishing a New Facility.......................... 223
Funding............................................224
Space Requirements............................. 225
Isolators, Equipment, and Housing..................226
Personnel...................................... 226
Identifying Staff............................ 227
Training.................................... 228
Retention..................................... 2 2 8
Fee Structure.................................. 229
Appendix: Sources for Equipment and Supplies...............231
Index.................................................. 233
Life Science
Gnotobiotic Mouse Technology
The popularity of germ-free animal models, particularly mice, for investigation of human physiology and disease has recently exploded. Gnotobiotic Mouse Technology: An Illustrated Guide provides the first manual for the maintenance, husbandry, and experimental manipulation of germ-free and gnotobiotic mice. It includes information on all aspects of establishing and operating a germ-free mouse research facility, from basic principles and equipment to detailed instructions for assembling and maintaining isolators, sterilizing supplies, handling animals, and monitoring sterility.
The book breaks down techniques and procedures into modules, each of which contains a step-by-step practical and visual guide to a set of related procedures. Each description consists of a materials list, introduction, and general overview, followed by a thoroughly illustrated walkthrough of the steps for each procedure. The descriptions end with troubleshooting tips. Technical chapters are heavily illustrated and include notations of potential pitfalls and alternatives. Other chapters discuss management procedures and practices such as the hiring and training of personnel, setting fee schedules, and record keeping.
This book is a landmark resource for establishing and maintaining a facility for gem-free mouse research. Useful to both technicians and investigators, it presents every step necessary to establish a successful facility. It also gives direction In expanding multi-user facilities and applying new technologies to your
current practices.
|
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| spelling | Vowles, Chriss J. 1983- Verfasser (DE-588)1095523694 aut Gnotobiotic mouse technology an illustrated guide Chriss J. Vowles, Natalie E. Anderson, Kathryn A. Eaton Boca Raton ; London ; New York CRC Press © 2016 2016 xvi, 237 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index mice cabt gnotobiotic animals cabt laboratory animals cabt Mice as laboratory animals Laboratory manuals. Germfree animals Laboratory manuals. Gnotobiotik (DE-588)4294572-0 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Gnotobiotik (DE-588)4294572-0 s Maus (DE-588)4169148-9 s DE-604 Anderson, Natalie E. 1986- Verfasser (DE-588)1095523724 aut Eaton, Kathryn A. aut Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028848110&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028848110&sequence=000002&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Vowles, Chriss J. 1983- Anderson, Natalie E. 1986- Eaton, Kathryn A. Gnotobiotic mouse technology an illustrated guide mice cabt gnotobiotic animals cabt laboratory animals cabt Mice as laboratory animals Laboratory manuals. Germfree animals Laboratory manuals. Gnotobiotik (DE-588)4294572-0 gnd Maus (DE-588)4169148-9 gnd |
| subject_GND | (DE-588)4294572-0 (DE-588)4169148-9 |
| title | Gnotobiotic mouse technology an illustrated guide |
| title_auth | Gnotobiotic mouse technology an illustrated guide |
| title_exact_search | Gnotobiotic mouse technology an illustrated guide |
| title_full | Gnotobiotic mouse technology an illustrated guide Chriss J. Vowles, Natalie E. Anderson, Kathryn A. Eaton |
| title_fullStr | Gnotobiotic mouse technology an illustrated guide Chriss J. Vowles, Natalie E. Anderson, Kathryn A. Eaton |
| title_full_unstemmed | Gnotobiotic mouse technology an illustrated guide Chriss J. Vowles, Natalie E. Anderson, Kathryn A. Eaton |
| title_short | Gnotobiotic mouse technology |
| title_sort | gnotobiotic mouse technology an illustrated guide |
| title_sub | an illustrated guide |
| topic | mice cabt gnotobiotic animals cabt laboratory animals cabt Mice as laboratory animals Laboratory manuals. Germfree animals Laboratory manuals. Gnotobiotik (DE-588)4294572-0 gnd Maus (DE-588)4169148-9 gnd |
| topic_facet | mice gnotobiotic animals laboratory animals Mice as laboratory animals Laboratory manuals. Germfree animals Laboratory manuals. Gnotobiotik Maus |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028848110&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028848110&sequence=000002&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
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