B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Gießen
VVB Laufersweiler Verlag
2015
|
| Edition: | 1. Auflage |
| Series: | Edition Scientifique
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 235 Seiten Diagramme |
| ISBN: | 9783835963528 |
Staff View
MARC
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| 245 | 1 | 0 | |a B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma |c eingereicht von Katharina von Ahn |
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| 264 | 1 | |a Gießen |b VVB Laufersweiler Verlag |c 2015 | |
| 300 | |a 235 Seiten |b Diagramme | ||
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| 502 | |b Dissertation |c Justus-Liebig-Universität Gießen |d 2015 | ||
| 650 | 7 | |a Cancer |2 cabt | |
| 650 | 7 | |a pancreas |2 cabt | |
| 650 | 7 | |a adenocarcinoma |2 cabt | |
| 650 | 7 | |a molecular biology |2 cabt | |
| 650 | 7 | |a animal experiments |2 cabt | |
| 650 | 7 | |a radiotherapy |2 cabt | |
| 650 | 7 | |a ligands |2 cabt | |
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Record in the Search Index
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| adam_text | TABLE OF CONTENTS
I.TABLE OF CONTENTS
I.TABLE OF CONTENTS 6
II. LIST OF ABBREVIATIONS 10
1. INTRODUCTION 15
1.1 THE ANATOMY AND PHYSIOLOGY OF THE PANCREAS 15
1.2 TUMORS OF THE PANCREAS 16
1.3 ETIOLOGY AND PROGRESSION OF PDAC 16
1.4 SYMPTOMS OF PDAC 17
1.5 DIAGNOSIS OF PDAC 18
1.6 THERAPY OF PDAC 19
1.6.1 SURGICAL THERAPY OF PANCREAS CANCER 20
1.6.2 RADIOTHERAPY OF PDAC 21
1.6.3 CHEMOTHERAPY OF PDAC 21
1.6.4 IMMUNE THERAPY 23
1.7 THE IMMUNE SYSTEM 24
1.8 CELLS OF THE IMMUNE SYSTEM 25
1.8.1 LYMPHOCYTES 25
1.8.2 DENDRITIC CELLS (DC) 27
1.8.3. NATURAL KILLER CELLS 28
1.8.4. IMMUNE REGULATION 28
1.8.4.1 REGULATORY T CELLS (TREG) 28
1.8.4.2 MYELOID DERIVED SUPPRESSOR CELLS (MDSC) 29
1.8.4.3 THE B7-H1 MOLECULE 31
1.9. THE GOAL OF THIS THESIS 32
2. MATERIAL AND METHODS 33
2.1 MATERIAL 33
2.1.1 MICE 33
6
HTTP://D-NB.INFO/1076049702
TABLE OF CONTENTS
2.1.2 PANCREASCARCINOMA CELLS 33
2.1.3 PHARMACEUTICAL PRODUCTS 33
2.1.4 ANTI-MOUSE ANTIBODIES FOR THE FLOW CYTOMETRY 34
2.1.5 LABORATORY EQUIPMENT 35
2.1.6 LABORATORY CONSUMABLES 36
2.1.7 LABORATORY SOLUTIONS 37
2.1.8 MEDIA AND BUFFERS 37
2.1.8.1 BUFFERS FOR THE FLOW CYTOMETRY 37
2.1.8.2 MEDIUM, BUFFER AND SOLUTIONS FOR THE CELL CULTURE 38
2.1.8.3 BUFFER FOR MACS 39
2.1.9 SOFTWARE 39
2.2 METHODS 39
2.2.1 ORTHOTOPIC MOUSE MODEL 39
2.2.2 TREATMENT OF THE MICE WITH IFNA, WITH 5-FU OR WITH THE COMBINATION
OF
IFNA+5-FU 40
2.2.3 PREPARATION OF TUMORS AND SPLEENS, COLLECTING BLOOD SAMPLES 40
2.2.4 PREPARATION OF A SINGLE-CELL SUSPENSION 41
2.2.5 FLOW CYTOMETRY 42
2.2.5.1 IMMUNOPHENOTYPING 42
2.2.5.2 EXTRACELLULAR STAINING 43
2.2.5.3 INTRACELLULAR STAINING 43
2.2.6 TREATMENT OF SPLENOCYTES IN CULTURE 44
2.2.7 INVESTIGATION OF MDSC SUPPRESSIVE ACTIVITY 44
2.2.7.1 CFSE CELL LABELING OF SPLENOCYTES 44
2.2.7.2. DIGESTION OF TUMORS 45
2.2.7.3 MAGNETIC ACTIVATED CELL SORTING (MACS) 45
2.2.7.4 COCULTURE 46
7
TABLE OF CONTENTS
2.2.8 LUMINEX (BIOPLEX) ASSAY 47
2.2.9 STATISTICAL ANALYSIS 47
3. RESULTS 49
3.1 EFFECTS OF CHEMO (5-FU) - AND IMMUNOTHERAPY (IFNA) ON THE
CONSTELLATION OF
LEUKOCYTE SUBSETS AND ON THE EXPRESSION OF B7-H1/B7-DC MOLECULES ON
SPLENOCYTES OF HEALTHY WT AND B7-H1 KO MICE IN IN VITRO CULTURES
49
3.1.1 ANALYSIS OF CD4
+
/CD8
+
T CELLS 49
3.1.2 ANALYSIS OF REGULATORY T CELLS (TREG) 71
3.1.3 ANALYSIS OF DENDRITIC CELLS (DC) 74
3.1.4 ANALYSIS OF GR-1
+
CD11B
+
CELLS 86
3.2 IN VIVO EFFECTS OF CHEMO (5-FU) - AND IMMUNOTHERAPY (IFNA) ON THE
CONSTELLATION OF LEUKOCYTE SUBSETS AND THE EXPRESSION OF B7-H1/B7-DC
MOLECULES
IN TUMOR-BEARING WT AND B7-H1 KO MICE 90
3.2.1 ANALYSIS OF CD4
+
/CD8
+
T CELLS 90
3.2.2 ANALYSIS OF MYELOID DERIVED SUPPRESSOR CELLS (MDSC) 112
3.2.3 ANALYSIS OF B7-H1/B7-DC EXPRESSION ON MDSC 125
3.2.4 ANALYSIS OF REGULATORY T CELLS (TREG) 129
3.2.5 ANALYSIS OF DENDRITIC CELLS (DC) 134
3.3 EFFECTS OF CHEMO (5-FU) - AND IMMUNOTHERAPY (IFNA) ON CYTOKINE
CONCENTRATION
IN BLOOD SERUM OF TUMOR-BEARING WT AND B7-H1 KO MICE 149
3.4 ROLE OF B7-H1 EXPRESSION ON THE FUNCTIONAL PHENOTYPE OF MDSC AND ON
THE
SENSITIVITY OF SPLENOCYTES TO MDSC MEDIATED SUPPRESSION 155
3.4.1 EFFECT OF B7-H1 EXPRESSION ON THE FUNCTIONAL PHENOTYPE OF MDSC 155
3.4.2 EFFECT OF B7-H1 EXPRESSION ON THE SENSITIVITY OF SPLENOCYTES TO
MDSC
MEDIATED SUPPRESSION 162
3.5 EFFECTS OF B7-H1 PRESENCE ON CYTOKINES PRODUCTION IN IN VITRO
COCULTURES OF WT I
B7-H1 KO SPLENOCYTES AND WT MDSC 169
3.6 EFFECTS OF CHEMO (5-FU) - AND IMMUNOTHERAPY (IFNA) ON TUMOR VOLUME
AND
METASTASIS RATE OF WT AND B7-H1 KO TUMOR-BEARING MICE 178
8
TABLE OF CONTENTS
3.7 EFFECTS OF CHEMO (5-FU) - AND IMMUNOTHERAPY (IFNA) ON THE SURVIVAL
RATE OF WT
AND B7-H1 KO TUMOR-BEARING MICE 184
3.8 ANALYSIS OF THE CORRELATION BETWEEN TUMOR VOLUME AND SPECIFIC IMMUNE
CELL
POPULATIONS 188
4. DISCUSSION 190
5. SUMMARY 205
6. ZUSAMMENFASSUNG 206
III. LIST OF LITERATURE 207
IV. LIST OF FIGURES 224
V. LIST OF TABLES 234
VI. ACKNOWLEDGEMENT 235
9
|
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| dewey-full | 630 |
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| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
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| indexdate | 2024-07-10T07:25:35Z |
| institution | BVB |
| isbn | 9783835963528 |
| language | English |
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| oclc_num | 959911973 |
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| owner_facet | DE-188 |
| physical | 235 Seiten Diagramme |
| publishDate | 2015 |
| publishDateSearch | 2015 |
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| publisher | VVB Laufersweiler Verlag |
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| spelling | Ahn, Katharina von Verfasser (DE-588)1076145612 aut B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma eingereicht von Katharina von Ahn 1. Auflage Gießen VVB Laufersweiler Verlag 2015 235 Seiten Diagramme txt rdacontent n rdamedia nc rdacarrier Edition Scientifique Dissertation Justus-Liebig-Universität Gießen 2015 Cancer cabt pancreas cabt adenocarcinoma cabt molecular biology cabt animal experiments cabt radiotherapy cabt ligands cabt Doktorarbeit Uni Wissenschaft (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe Ahn, Katharina von B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028844259&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ahn, Katharina von B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma Cancer cabt pancreas cabt adenocarcinoma cabt molecular biology cabt animal experiments cabt radiotherapy cabt ligands cabt |
| subject_GND | (DE-588)4113937-9 |
| title | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma |
| title_auth | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma |
| title_exact_search | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma |
| title_full | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma eingereicht von Katharina von Ahn |
| title_fullStr | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma eingereicht von Katharina von Ahn |
| title_full_unstemmed | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma eingereicht von Katharina von Ahn |
| title_short | B7-H1 as a new molecular target for the treatment of pancreatic adenocarcinoma |
| title_sort | b7 h1 as a new molecular target for the treatment of pancreatic adenocarcinoma |
| topic | Cancer cabt pancreas cabt adenocarcinoma cabt molecular biology cabt animal experiments cabt radiotherapy cabt ligands cabt |
| topic_facet | Cancer pancreas adenocarcinoma molecular biology animal experiments radiotherapy ligands Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028844259&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ahnkatharinavon b7h1asanewmoleculartargetforthetreatmentofpancreaticadenocarcinoma |