Synthetic mRNA: production, introduction into cells, and physiological consequences
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2016]
|
| Schriftenreihe: | Methods in molecular biology
1428 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xii, 320 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493936236 |
Internformat
MARC
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| 245 | 1 | 0 | |a Synthetic mRNA |b production, introduction into cells, and physiological consequences |c edited by Robert E. Rhoads |
| 264 | 1 | |a New York, NY |b Humana Press |c [2016] | |
| 264 | 4 | |c © 2016 | |
| 300 | |a xii, 320 Seiten |b Illustrationen, Diagramme | ||
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Datensatz im Suchindex
| _version_ | 1804176016433741824 |
|---|---|
| adam_text | Titel: Synthetic mRNA
Autor: Rhoads, Robert E
Jahr: 2016
Contents
Preface..................................................................... v
Contributors................................................................. ix
Part I Introduction
1 Synthetic mRNA: Production, Introduction into Cells,
and Physiological Consequences.......................................... 3
Robert E. Rhoads
Part II Synthesis of mRNA
2 Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking
Experiments............................................................ 31
Joanna Kowalska, Franck Martin, and Jacek Jemielity
3 Enzymatic Modification of 5 -Capped RNA and Subsequent
Labeling by Click Chemistry............................................. 45
Josephin M. Holstein, Daniela Stummer, and Andrea Rentmeister
4 Preparation of Functional, Fluorescently Labeled mRNA
Capped with Anthraniloyl-m7GpppG...................................... 61
Artem V Domashevskiy, David J. Rodriguez,
Dilantha Gunawardana, and Dixie J. Goss
5 Intronless p-Globin Reporter: A Tool for Studying Nuclear
RNA Stability Elements.................................................. 77
Jessica A. Brown and Joan A. Steitz
6 Synthetic mRNA with Superior Properties that Mimics
the Intracellular Fates of Natural Histone mRNA........................... 93
Wei Su, Michael K. Slevin, William F. Marzluff, and Robert E. Rhoads
7 Engineering WT1-Encoding mRNA to Increase Translational
Efficiency in Dendritic Cells.............................................. 115
Daphne Benteyn, Carlo Heirman, Kris Thielemans, and Aude Bonehill
Part III Introduction of Synthetic mRNA into Cells
8 Electroporation of Alphavirus RNA Translational Reporters
into Fibroblastic and Myeloid Cells as a Tool to Study
the Innate Immune System............................................... 127
Christina L. Gardner, Derek W. Trobaugh, Kate D. Ryman,
and William B. Klimstra
9 GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use....... 139
Judith Derdelinckx, Zwi N. Berneman, and Nathalie Cools
vii
viii
Contents
10 Large-Scale mRNA Transfection of Dendritic Cells by Electroporation
in Continuous Flow Systems............................................. 151
David Selmeczi, Thomas Steen Hansen, Ozcan Met,
Inge Marie Svane, and Niels B. Larsen
11 FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines.............. 163
Sebastian Kreiter, Mustafa Diken, Abderraouf Selmi,
Jutta Petschenka, Ozlem Tureci, and Ugur Sahin
12 Transfecting Human Monocytes with RNA................................ 177
Jens Dannull and Smita K. Nair
13 In Vitro Synthesis, Delivery, and Bioavailability of Exogenous
mRNA in Gene Transfer Mediated by PiggyBac Transposition................ 187
Solenne Bire, Nicole Ishac, and Florence Rouleux-Bonnin
14 Transfection of Human Keratinocytes with Nucieoside-Modified
mRNA Encoding CPD-Photolyase to Repair DNA Damage................. 219
Gdbor Boros, Katalin Kariko, Hiromi Muramatsu,Edit Miko,
Eszter Emri, Csaba Hegedus, Gabriella Emri, and Eva Remenyik
Part IV Alteration of Cellular Function with Synthetic mRNA
15 Delivery of Synthetic mRNA Encoding FOXP3 Antigen
into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy......... 231
Gayathri R. Devi and Sritama Nath
16 Immune Monitoring Using mRNA-Transfected Dendritic Cells.............. 245
Troels Holz Borch, Inge Marie Svane, and Ozcan Met
17 Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2..... 261
Manja Idorn, Per thor Straten, Inge Marie Svane, and Ozcan Met
18 mRNA Electroporation of Dendritic Cells with WT1, Survivin,
and TriMix (a Mixture of caTLR4, CD40L, and CD70)..................... 277
An Coosemans, Sandra Tuyaerts, Kim Morias, Jurgen Corthals,
Carlo Heirman, Kris Thielemans, Stefaan W. Van Gool,
Ignace Vergote, and Frederic Amant
19 Redirecting T Cell Specificity Using T Cell Receptor Messenger
RNA Electroporation.................................................... 285
Sarene Koh, Noriko Shimasaki, and Antonio Bertoletti
20 Measuring Hematocrit in Mice Injected with In Vitro-Transcribed
Erythropoietin mRNA................................................... 297
Azita Josefine Mahiny and Katalin Kariko
21 Genetic Modification of Human Pancreatic Progenitor Cells
Through Modified mRNA............................................... 307
Song Lu, Christie C. Chow, Junwei Zhou, Po Sing Leung,
Stephen K. Tsui, and Kathy O. Lui
Index...................................................................... 319
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| spelling | Synthetic mRNA production, introduction into cells, and physiological consequences edited by Robert E. Rhoads New York, NY Humana Press [2016] © 2016 xii, 320 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1428 Springer protocols Rhoads, Robert E. 1944- (DE-588)122674170 edt Erscheint auch als Online-Ausgabe 978-1-4939-3625-0 Methods in molecular biology 1428 (DE-604)BV035362695 1428 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028840203&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Synthetic mRNA production, introduction into cells, and physiological consequences Methods in molecular biology |
| title | Synthetic mRNA production, introduction into cells, and physiological consequences |
| title_auth | Synthetic mRNA production, introduction into cells, and physiological consequences |
| title_exact_search | Synthetic mRNA production, introduction into cells, and physiological consequences |
| title_full | Synthetic mRNA production, introduction into cells, and physiological consequences edited by Robert E. Rhoads |
| title_fullStr | Synthetic mRNA production, introduction into cells, and physiological consequences edited by Robert E. Rhoads |
| title_full_unstemmed | Synthetic mRNA production, introduction into cells, and physiological consequences edited by Robert E. Rhoads |
| title_short | Synthetic mRNA |
| title_sort | synthetic mrna production introduction into cells and physiological consequences |
| title_sub | production, introduction into cells, and physiological consequences |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028840203&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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