Receptor and ion channel detection in the brain: methods and protocols
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| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York
Humana Press
[2016]
|
| Series: | Neuromethods
110 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xviii, 475 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493930630 9781493930647 |
Staff View
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| 245 | 1 | 0 | |a Receptor and ion channel detection in the brain |b methods and protocols |c edited by Rafael Luján, Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla-La Mancha, Albacete, Spain; Francisco Ciruela, Pharmacology Unit, Department of Pathology and Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain |
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Record in the Search Index
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| adam_text | Contents
Series Preface................................................................. v
Preface...................................................................... vii
Contributors.................................................................. XV
Part I Molecular Techniques
1 Production of High-Quality Antibodies for the Study
of Receptors and Ion Channels.............................................. 3
Masahiko Watanabe
2 Co-immunoprecipitation from Brain......................................... 19
Xavier Moratóy Dastel O. Borroto-Escuelay Kjell Fuxey
Victor Fernândez-Duenasy and Francisco Ciruela
3 Subsynaptic Membrane Fractionation........................................ 31
Paula M. Canas and Rodrigo A. Cunha
4 Investigation of Neurotransmitter Receptors in Brain Slices
Using Cell Surface Biotinylation.......................................... 39
Elek Molnar
5 Single Nanoparticle Tracking of Surface Ion Channels
and Receptors in Brain Cells.......................................... 49
Juan Varelay Julien Dupuisy and Laurent Groc
6 Radioligand Binding Detection of Receptors in Brain Membranes......... 59
Fuencisla Pilar-Cuéllary Alvaro DtaZy Emilio Garro-MartineZy
Alicia Martiny Beatriz Romeroy and Elsa M. Valdizdn
7 Recombinant Alphavirus-Mediated Expression of Ion Channels
and Receptors in the Brain................................................ 77
Markus U. Ehrengruber and Kenneth Lundstrom
8 Fluorescent Ligands and TR-FRET to Study Receptor-Receptor
Interactions in the Brain................................................. 99
Victor Ferndndez-DuehaSy Thierry Durrouxy and Francisco Ciruela
9 In Situ Proximity Ligation Assay to Study and Understand
the Distribution and Balance of GPCR Homo- and Heteroreceptor
Complexes in the Brain................................................... 109
Dastel O. Borroto-Escuelay Beth Hagmany Miles Woolfendeny
Luca Pintony Antonio Jimenez-Beristainy Julia Oflijany
Manuel NarvaeZy Michael Di Palmay Kristin Feltmanny
Stfano Sartiniy Patrizia Ambroginiy Francisco Ciruelay
Riccardo Cuppinty and Kjell Fuxe
XII
Contents
Part II Neuroanatomical Techniques
10 Fluorescent In Situ Hybridization for Sensitive and Specific Labeling. 127
Miwako Yamasaki and Masahiko W tanabe
11 Autoradiographic Visualization of G Protein-Coupled Receptors in Brain .... 143
Rebeca Vidal, Raquel Linge, María Josefa Castillo, Josef Amigó,
Elsa M. Valdizdn, and Elena Castro
12 Analysis of the Expression Profile and Regional Distribution
of Neurotransmitter Receptors and Ion Channels
in the Central Nervous System Using Histoblots........................ 157
Elek Molnar
13 Immunohistochemistry for Ion Channels and Their Interacting
Molecules: Tips for Improving Antibody Accessibility.................. 171
Kohtarou Konno and Masahiko Watanabe
14 Localization of GFP-Tagged Proteins at the Electron Microscope........ 179
Sara Gil-Perotin, A. Cebridn-Sillay V Herranz-Pérez,
P. García-Belda, S. Gil-García, M. Fil, J.S. Lee, M.V. Nachury,
and José Manuel García-Verdugo
15 Pre-embedding Methods for the Localization
of Receptors and Ion Channels......................................... 191
Rafael Lujan
16 Post-embedding Immunohistochemistry in the Localisation
of Receptors and Ion Channels......................................... 211
Rafael Lujan and Masahiko Watattabe
17 High-Resolution Localization of Membrane Proteins by SDS-Digested
Freeze-Fracture Replica Labeling (SDS-FRL)............................ 233
Harumi Harada and Ryuichi Shiøemoto
18 Application of Virus Vectors for Anterograde Tract-Tracing
and Single-Neuron Labeling Studies.................................... 247
Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta
19 Analysis of Synaptic Connections at the Electron Microscopic Level
Using Viral Vectors..................................................... 267
Takahiro Furuta, Keiko Okamoto-Furuta, and Hiroyuki Hioki
20 Morphological and Neurochemical Characterization
of Electrophysiologically Identified Cells.............................. 277
Yoshiyuki Kubota
Part III Functional Techniques
21 Using Electrophysiology to Study Synaptic and Extrasynaptic
Ionotropic Receptors in Hippocampal Neurons........................... 313
Ian D. Coombs and David Soto
22 Biophysical Methods to Analyze Direct G֊Protein Regulation
of Neuronal Voltage-Gated Calcium Channels.............................. 357
Norbert Weiss and Michel De Waard
Contents xiü
23 Electrophysiological Recordings in Behaving Animals..................... 369
Agnes Gruart and José M. Delgado-García
24 Voltammetry in Behaving Animals......................................... 397
Kendra D. Banner and George V. Rebec
25 In Vivo Brain Microdialysis of Monoamines............................... 415
Jorge E. Ortega, ƒ. Javier Meana, and Luis F. Callado
26 Calcium Transients in Single Dendrites and Spines
of Pyramidal Neurons In Vitro........................................... 435
Rhiannon M. Meredith and Martine R. Groen
27 Dynamic Recording of Membrane Potential from Hippocampal
Neurons by Using a FRET՝Based Voltage Biosensor....................... 447
Víctor Ferná ndez-DueñaSy Xavier Morató Thomas Knopf el,
and Francisco Ciruela
28 Determination of GPCR-Mediated cAMP Accumulation
in Rat Striatal Synaptosomes.......................................... 455
Jaume Taura, Víctor Fernández-Dueñas, and Francisco Ciruela
29 GPCR-Mediated MAPK/ERK Cascade Activation
in Mouse Striatal Slices............................................... 465
Maricel Gómez-Soler Víctor Fernández-Dueñas, and Francisco Ciruela
Subject Index.............................................................. 473
|
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| language | English |
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| series | Neuromethods |
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| spelling | Receptor and ion channel detection in the brain methods and protocols edited by Rafael Luján, Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla-La Mancha, Albacete, Spain; Francisco Ciruela, Pharmacology Unit, Department of Pathology and Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain New York Humana Press [2016] xviii, 475 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Neuromethods 110 Springer Protocols Ionenkanal (DE-588)4138699-1 gnd rswk-swf Gehirn (DE-588)4019752-9 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Rezeptor (DE-588)4049722-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Gehirn (DE-588)4019752-9 s Ionenkanal (DE-588)4138699-1 s Rezeptor (DE-588)4049722-7 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Luján, Rafael edt Ciruela, Francisco edt Neuromethods 110 (DE-604)BV008920602 110 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028752244&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Receptor and ion channel detection in the brain methods and protocols Neuromethods Ionenkanal (DE-588)4138699-1 gnd Gehirn (DE-588)4019752-9 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd Rezeptor (DE-588)4049722-7 gnd |
| subject_GND | (DE-588)4138699-1 (DE-588)4019752-9 (DE-588)7650063-9 (DE-588)4049722-7 (DE-588)4143413-4 |
| title | Receptor and ion channel detection in the brain methods and protocols |
| title_auth | Receptor and ion channel detection in the brain methods and protocols |
| title_exact_search | Receptor and ion channel detection in the brain methods and protocols |
| title_full | Receptor and ion channel detection in the brain methods and protocols edited by Rafael Luján, Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla-La Mancha, Albacete, Spain; Francisco Ciruela, Pharmacology Unit, Department of Pathology and Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain |
| title_fullStr | Receptor and ion channel detection in the brain methods and protocols edited by Rafael Luján, Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla-La Mancha, Albacete, Spain; Francisco Ciruela, Pharmacology Unit, Department of Pathology and Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain |
| title_full_unstemmed | Receptor and ion channel detection in the brain methods and protocols edited by Rafael Luján, Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of Medicine, University of Castilla-La Mancha, Albacete, Spain; Francisco Ciruela, Pharmacology Unit, Department of Pathology and Pathology and Experimental Therapeutics, School of Medicine, IDIBELL, University of Barcelona, Barcelona, Spain |
| title_short | Receptor and ion channel detection in the brain |
| title_sort | receptor and ion channel detection in the brain methods and protocols |
| title_sub | methods and protocols |
| topic | Ionenkanal (DE-588)4138699-1 gnd Gehirn (DE-588)4019752-9 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd Rezeptor (DE-588)4049722-7 gnd |
| topic_facet | Ionenkanal Gehirn Molekularbiologische Methode Rezeptor Aufsatzsammlung |
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