NLR proteins: methods and protocols
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
[2016]
|
| Schriftenreihe: | Methods in molecular biology
1417 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xi, 256 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493935642 |
Internformat
MARC
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| 020 | |a 9781493935642 |9 978-1-4939-3564-2 | ||
| 035 | |a (OCoLC)951731750 | ||
| 035 | |a (DE-599)BVBBV043331952 | ||
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| 245 | 1 | 0 | |a NLR proteins |b methods and protocols |c edited by Francesco Di Virgilio, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy; Pablo Pelegrín, Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Murcia, Spain |
| 264 | 1 | |a New York |b Humana Press |c [2016] | |
| 264 | 4 | |c 2016 | |
| 300 | |a xi, 256 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 1417 | |
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| 830 | 0 | |a Methods in molecular biology |v 1417 |w (DE-604)BV035362695 |9 1417 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-028752107 | ||
Datensatz im Suchindex
| _version_ | 1804175878418071552 |
|---|---|
| adam_text | Titel: NLR proteins
Autor: Di Virgilio, Francesco
Jahr: 2016
Contents
Preface............................................................. v
Contributors............................................................. ix
1 Innate Immune Receptors........................................... 1
Natalia Munoz-Wolf and Ed C. Lavelle
2 Atypical Inflammasomes............................................. 45
Ann M. Janowski and Fayyaz S. Sutterwala
3 Assessment of Inflammasome Activation by Cytokine
and Danger Signal Detection......................................... 63
Nicolas Riteau, AuHlie Gombault, and Isabelle Couillin
4 Investigating IL-lß Secretion Using Real-Time Single-Cell Imaging............ 75
Catherine Diamond, James Bagnall, David G. Spiller, Michael R. White,
Alessandra Mortellaro, Pawel Paszek, and David Brough
5 Measuring IL-lß Processing by Bioluminescence Sensors I:
Using a Bioluminescence Resonance Energy Transfer Biosensor............... 89
Vincent Compan and Pablo Pelegrin
6 Measuring IL-lß Processing by Bioluminescence Sensors II: The iGLuc System ... 97
Eva Bartok, Maria Kampes, and Veit Hornung
7 Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System...... 115
Francesco Di Virgilio, Paolo Pinton, and Simonetta Falzoni
8 Measuring NLR Oligomerization I: Size Exclusion Chromatography,
Co-immunoprecipitation, and Cross-Linking............................. 131
Sonal Khare, Alexander D. Radian, Andrea Dorfleutner,
and Christian Stehlik
9 Measuring NLR Oligomerization II: Detection of ASC Speck
Formation by Confocal Microscopy and Immunofluorescence................ 145
Michael Beilharz, Dominic De Nardo s, Eicke Latz, and Bernardo S. Franklin
10 Measuring NLR Oligomerization III: Detection of NLRP3 Complex
by Bioluminescence Resonance Energy Transfer........................... 159
Fdtima Martin-Sdnchez, Vincent Compan, and Pablo Pelegrin
11 Measuring NLR Oligomerization IV: Using Förster Resonance Energy
Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM)
to Determine the Close Proximity of Inflammasome Components............. 169
Catrin Youssif, Bdrbara Flix, Olivia Belbin, and Mbnica Comalada
12 Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay........... 185
Yung-Hsuan Wu and Ming-Zong Lai
13 Assessing Caspase-1 Activation........................................ 197
Baptiste Guey and Virginie Petrilli
14 Cell-Free Assay for Inflammasome Activation............................. 207
Yvan Jamilloux and Fabio Martinon
vii
viii
Contents
15 Functional Reconstruction of NLRs in HEK293 Cells...................... 217
Vincent Cornpan and Gloria Ldpez-Casteßn
16 Method to Measure Ubiquitination of NLRs............................. 223
Pablo Palazön-Riquelme and Gloria Ldpez-Casteßn
17 Cytofluorometric Quantification of Cell Death Elicited by NLR Proteins........ 231
Valentina Sica, Gwenola Manic, Guido Kroemer, Ilio Vitale,
and Lorenzo Galluzzi
18 NLR in Human Diseases: Role and Laboratory Findings..................... 247
Sonia Carta, Marco Gattorno, and Anna Rubartelli
Index................................................................... 255
|
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| dewey-ones | 572 - Biochemistry |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T07:23:17Z |
| institution | BVB |
| isbn | 9781493935642 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028752107 |
| oclc_num | 951731750 |
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| owner_facet | DE-11 DE-19 DE-BY-UBM |
| physical | xi, 256 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | NLR proteins methods and protocols edited by Francesco Di Virgilio, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy; Pablo Pelegrín, Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Murcia, Spain New York Humana Press [2016] 2016 xi, 256 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1417 Di Virgilio, Francesco edt Pelegrín, Pablo edt Erscheint auch als Online-Ausgabe 978-1-4939-3566-6 Methods in molecular biology 1417 (DE-604)BV035362695 1417 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028752107&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | NLR proteins methods and protocols Methods in molecular biology |
| title | NLR proteins methods and protocols |
| title_auth | NLR proteins methods and protocols |
| title_exact_search | NLR proteins methods and protocols |
| title_full | NLR proteins methods and protocols edited by Francesco Di Virgilio, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy; Pablo Pelegrín, Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Murcia, Spain |
| title_fullStr | NLR proteins methods and protocols edited by Francesco Di Virgilio, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy; Pablo Pelegrín, Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Murcia, Spain |
| title_full_unstemmed | NLR proteins methods and protocols edited by Francesco Di Virgilio, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy; Pablo Pelegrín, Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Murcia, Spain |
| title_short | NLR proteins |
| title_sort | nlr proteins methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028752107&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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